LOCUS       sequence-81579-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Fischbach NA, Rozenfeld S, Shen W, Fong S, Chrobak D, Ginzinger D, 
            Kogan SC, Radhakrishnan A, Le Beau MM, Largman C, Lawrence HJ
  TITLE     HOXB6 overexpression in murine bone marrow immortalizes a 
            myelomonocytic precursor in vitro and causes hematopoietic stem cell
            expansion and acute myeloid leukemia in vivo.
  JOURNAL   Blood. 2005 Feb 15. 105(4):1456-66.
  PUBMED    15522959
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2005
            Feb 15. 105(4):1456-66."
COMMENT     Sequence Label: MEIS1A-MIY
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(132..134,135..137,138..851)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(177..198)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(438..457)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     785..806
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
       61 ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
      121 atgataatac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      181 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      241 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      301 cctggcccac cctcgtgacc accttcggct acggcctgca gtgcttcgcc cgctaccccg
      361 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      421 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
      481 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
      541 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
      601 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
      661 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
      721 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
      781 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
      841 tgtacaagta aagcggcccg ataaaataaa agattttatt tagtctccag aaaaa
//
