LOCUS       sequence-81580-e        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  transient expression of pcoCas9 gene in plant cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Li JF, Norville JE, Aach J, McCormack M, Zhang D, Bush J, Church GM,
            Sheen J
  TITLE     Multiplex and homologous recombination-mediated genome editing in 
            Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9.
  JOURNAL   Nat Biotechnol. 2013 Aug;31(8):688-91. doi: 10.1038/nbt.2654.
  PUBMED    23929339
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2013-08"; volume: "31"; issue:
            "8"; pages: "688-91"
COMMENT     Sequence Label: HBT-pcoCas9
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          837..1025
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
ORIGIN
        1 atgttctctg gcttgtgtct acccataacc ttaacaagct catcaacaac cttaacggtc
       61 tgaaggattc ccttcttgat agctggagat ccagcaaggt tagcgatgtg ctcgtgaaga
      121 gaatctccct gtccagaaac ctgagccttc tggatgtcct ccttgaaggt aagagaatca
      181 tcgtggataa gctgcatgaa gtttctgtta gcgaatccat cagacttcaa gaaatcaagg
      241 atggtctttc cagactgctt atctctgatt ccgttgatca acttacgaga aagtctaccc
      301 catccggtgt atcttcttct cttcaactgc ttcataacct tatcatcgaa aaggtgagcg
      361 taggtcttga gtctctcctc gatcatctct ctatcctcga aaagggtaag ggtaagaacg
      421 atgtcctcaa ggatgtcctc gttctcctcg ttatcaagga aatccttatc cttgatgatc
      481 ttcaaaagat cgtggtaggt tccaagagaa gcgttgaatc tatcctcaac tccagagatt
      541 tcaacagaat cgaagcactc gatcttcttg aagtaatcct ccttcaactg cttaacggta
      601 acctttctgt tggtcttgaa aagaagatca acgatagcct tcttctgctc tccagaaagg
      661 aaagctggct ttctcattcc ctcggtaacg tacttaacct tggtaagctc gttgtaaacg
      721 gtgaagtact cgtaaagaag agagtgcttt ggaagaacct tctcgtttgg aaggttctta
      781 tcgaagttgg tcattctctc gatgaaagac tgagcagaag ctcccttatc aacaacctgc
      841 acatcaacaa attttggtca tatattagaa aagttataaa ttaaaatata cacacttata
      901 aactacagaa aagcaattgc tatatactac attcttttat tttgaaaaaa atatttgaaa
      961 tattatatta ctactaatta atgataatta ttatatatat atcaaaggta gaagcagaaa
     1021 cttacctcct cgaa
//
