LOCUS       sequence-81581-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Jackson EL, Willis N, Mercer K, Bronson RT, Crowley D, Montoya R, 
            Jacks T, Tuveson DA
  TITLE     Analysis of lung tumor initiation and progression using conditional 
            expression of oncogenic K-ras.
  JOURNAL   Genes Dev. 2001 Dec 15. 15(24):3243-8.
  PUBMED    11751630
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2001 Dec 15. 15(24):3243-8."
COMMENT     Sequence Label: LSL K-ras G12D
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    552..585
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      616..625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(661..680)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gatatctttt tcaaagcggc tggctgccgt cctttacaag cgcacgcaga ctgtagagca
       61 gcgttacctc tatcgtaggg tcgtactcat ccacaaagtg attctgaatt agctgtatcg
      121 tcaaggcgct cttgcctacg ccatcagctc caaccaccac aagcttatac tcagtcattt
      181 tcagcaggcc ttacaataaa aataataaaa tacaactaaa ttagaacatg tctcacacaa
      241 gattatcaaa aactttatca atattttaac actcaccttt gtgtgtaaga ctctaagata
      301 ttccgaattc agtgactaca gatgtacaga gtaacctgta agtcacttac aactttctca
      361 tcaacactta gtaaaaatac atggaaacca gtactttcat cttctatcat ttagcttgtt
      421 gatctaaaca gccagattac tgttttgtag cagctaatgg ctctcaaagg aatgtatcat
      481 gacttcactc agtacaaata ttctgcatag tacgctatac cctgtggaca cacccgcatg
      541 agcttgtcga cataacttcg tataatgtat gctatacgaa gttattaggt ccctcgacct
      601 gcagacttac tcaagccatg gtggctagct tgcggaattc cagacatgat aagatacatt
      661 gatgagtttg gacaaaccac aacta
//
