LOCUS       sequence-81585-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant 
            expression of Cas9 and multiple cloning sites for insertion of guide
            RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  TITLE     Sheen lab CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFGC-pcoCas9
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(324..344)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(351..398)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     promoter        complement(join(410..597,598..620,621..919))
                     /label=35SPPDK promoter
                     /note="hybrid promoter consisting of the cauliflower mosaic
                     virus 35S enhancer fused to the maize C4PPDK basal promoter
                     (Yoo et al., 2007)"
ORIGIN
        1 agtctgtgga agaaagaatc atcaacctta gccatctcgt tagagaagat ttcctgaagg
       61 tagcagattc tgttctttct tctggtgtat cttcttctag cggttctctt caatctggta
      121 gcctcagcgg tctctccaga atcgaaaaga agagcaccga taaggttctt cttgatagag
      181 tgtctatcgg tgtttccaag aaccttgaac ttcttagatg gaaccttgta ctcatcggtg
      241 ataacagccc atccaacaga gttggttccg atgtcaagtc cgatagagta cttcttatca
      301 gcagctggaa ctccgtggat tccaaccttt ctcttcttct ttggagccat cttatcatca
      361 tcatccttgt aatccttatc atcatcatcc ttgtaatcca tggatccacg gagcaagggg
      421 agagggagcc gagagctgct tactacgtga ccgccccgcg cctgctgctg tccggagctc
      481 caacggagct ggagaggggg tgtgtgtggc gagagagtac agcgaaaagg tgagaggcga
      541 acgggtgaat cctgcacgcg gaatgccttt attcttatac catccgtggg gcgcgaccca
      601 agcttgggcc caagcttggg gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt
      661 ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
      721 gatgctcctt gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
      781 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
      841 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
      901 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
      961 gg
//
