LOCUS       sequence-81593-e        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant 
            expression of Cas9 and multiple cloning sites for insertion of guide
            RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  TITLE     Sheen lab CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFGC-pcoCas9
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          833..1021
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
ORIGIN
        1 tctctggctt gtgtctaccc ataaccttaa caagctcatc aacaacctta acggtctgaa
       61 ggattccctt cttgatagct ggagatccag caaggttagc gatgtgctcg tgaagagaat
      121 ctccctgtcc agaaacctga gccttctgga tgtcctcctt gaaggtaaga gaatcatcgt
      181 ggataagctg catgaagttt ctgttagcga atccatcaga cttcaagaaa tcaaggatgg
      241 tctttccaga ctgcttatct ctgattccgt tgatcaactt acgagaaagt ctaccccatc
      301 cggtgtatct tcttctcttc aactgcttca taaccttatc atcgaaaagg tgagcgtagg
      361 tcttgagtct ctcctcgatc atctctctat cctcgaaaag ggtaagggta agaacgatgt
      421 cctcaaggat gtcctcgttc tcctcgttat caaggaaatc cttatccttg atgatcttca
      481 aaagatcgtg gtaggttcca agagaagcgt tgaatctatc ctcaactcca gagatttcaa
      541 cagaatcgaa gcactcgatc ttcttgaagt aatcctcctt caactgctta acggtaacct
      601 ttctgttggt cttgaaaaga agatcaacga tagccttctt ctgctctcca gaaaggaaag
      661 ctggctttct cattccctcg gtaacgtact taaccttggt aagctcgttg taaacggtga
      721 agtactcgta aagaagagag tgctttggaa gaaccttctc gtttggaagg ttcttatcga
      781 agttggtcat tctctcgatg aaagactgag cagaagctcc cttatcaaca acctgcacat
      841 caacaaattt tggtcatata ttagaaaagt tataaattaa aatatacaca cttataaact
      901 acagaaaagc aattgctata tactacattc ttttattttg aaaaaaatat ttgaaatatt
      961 atattactac taattaatga taattattat atatatatca aaggtagaag cagaaactta
     1021 cctcctcgaa gttccaaggg gtga
//
