LOCUS       sequence-81597-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of E. coli CRP/CAP for protein purification 
            (Intein-chiting binding domain fussion, New England Biolabs pTXB1 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  TITLE     Plamids for protein purification in E. coli of superfolder GFP, 
            CRP/CAP (E. coli), FIS (E. coli), CI (lambda phage) and CII (lambda 
            phage)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTXB1-CRP/CAP
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..185)
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             complement(216..809)
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
ORIGIN
        1 ttagcagccg gatccccttc ctgcagtcat tgaagctgcc acaaggcagg aacgttggat
       61 ggttcccatc ctgccaagga ggtgtggggc tgcaaacatt tatacgtctt gccgttatat
      121 gtgaccaatt gtcccgcagt ataagctgtg ttgacctgcc aagcggatac accaggattt
      181 gtcgtgaggc ctgagttcag accggtgagg ccagtagcgt ggctgacgaa cccgttcgtg
      241 ataaacgcgt ggtctgccgt gtcgacacga aggctataca ccggctgcac gccggcgtcg
      301 gtgacactgg cgactttcgc gtagtagaac cgcccgtcgg tcagctcgtc ggcgatagct
      361 tgggcgtccg ggtctcggtg gtgtgcttcc aagaaacgca ccagtccagg gacgccgact
      421 gtgtaggttg tgggcgcaaa ttcgggtttc ccgcgggcaa aacctgcaca gtcgacgctg
      481 aatgcgctgc gttgaatcac cgcgtaatcg cccggcttga tttcgtcgat cagcttccac
      541 agcagggtcg gcaccccggc gacgtcgacc aaacacaaca acgggtggtt cgcggtgccc
      601 gtcacacgca gaccttcgac cgtacgcacc gtgtacaccg gatgctcgcc ggagtggaac
      661 agccggtcgg cgagcacggg attgccatgc cggtcaagga ctttcaggtc gatggcgttg
      721 tcactgttgg gccgcgcacc cggcacgatg tcggcgatgc gtaccgactc gccctcgggt
      781 agggcaacta gtgcatctcc cgtgatgcaa cgagtgccgt aaacgacgat ggttttaccg
      841 tgtgcggaga tcaggttctg atcttccagc atcttcagaa tgcgtcc
//
