LOCUS       sequence-8160-ex        9073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Mayr C, Hemann MT, Bartel DP
  TITLE     Disrupting the pairing between let-7 and Hmga2 enhances oncogenic 
            transformation.
  JOURNAL   Science. 2007 Mar 16. 315(5818):1576-9.
  PUBMED    17322030
REFERENCE   2  (bases 1 to 9073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2007 Mar 16. 315(5818):1576-9."
COMMENT     Sequence Label: pcDNA3.1 Hmga2 ORFtr
FEATURES             Location/Qualifiers
     source          1..9073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4667..4684)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4673..4897
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4943..5371
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5030..5049)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5240..5261
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5380..5400)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5385..5714
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5565..5700
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5627..5646
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5781..6575
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5835..5854)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6445..6464
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6749..6870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6786..6805)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6840..6859
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6919..6935)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6919..6935)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6932..6954)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6943..6959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6967..6973,6974..6991,6992..6997))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7012..7033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7150..7167)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7321..7906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7401..7420)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8077..8868,8869..8937))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8700..8719
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8938..9042)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcccg
      961 ggatcgcaac tcccgagcct ttgcggagag agcaaccctc tccgcctcca actcttccct
     1021 ctccttcgct tcccgcctcc tctccctacc tccacctcta cctccgccac ccactgcccg
     1081 cagcgcctcc tcctttcctc ctcctcctct cctcctcctc tcttcctcct cccctctctc
     1141 tttttggcag ccgctggacg tccggtgttg atggtggcag cggcggcagc ctaagcagca
     1201 gcagtagtcc ccggcgcact cgccagctcg cctgctctcg ccgctcttgc cctctccagc
     1261 tctctacatc ccgtctcccg aaaggtgctg ggcggattcg gggcggcgga ggccgagcgg
     1321 ctgcagcggc ggtaccggta gaggcagtgg tagcggcggc gggaggcagg atgagcgcac
     1381 gcggtgaggg cgccgggcag ccgtccacat cagcccaggg acaacctgcc gccccggtgc
     1441 cacagaagcg aggacgcggc cgacccagga agcagcagca agagccaacc tgtgagccct
     1501 ctcctaagag acccagagga agacccaaag gcagcaaaaa caagagcccc tctaaagcag
     1561 cccagaagaa agcagagacc attggagaaa aacggccaag aggcagacct aggaaatggt
     1621 agggggcgcc gacattcaat ttctacctca gcatcagttg gatcttttga agggagaaga
     1681 cactgcagtg accagttatt cttaactgcc acggtctttc tacttcctgc ggggtggggc
     1741 gggggcgggg ctgggcgagg ggcggggccg gggtgggcga aatcgcataa ccttgagaag
     1801 gactatatta atcactttgt aatcccttca cagtcccagg tttagtgaaa aactgctgta
     1861 aacacggggg acacagttta acaatgcaac ttttaatgac tgttttcttt ttccttaact
     1921 tactaatagt ttgtggatct gataagcaag ggtgtgtggt tgaagaaaac ctctgtggtg
     1981 ggcttaatca gtcactacat gcaaacccta aaccggcacc ctggtgaccg ggggcattcg
     2041 tataagaaaa gcattgtgtg tgactctgtg tccactcaga tgccaccccc accatgatca
     2101 tagaaaatct gcttaggaca ccaaagatga gaactagaca ctactctcct ttctttgtgt
     2161 ataatcttgt agacacttac ttgatttttt tttctttttt tacttttcaa ttctgaatga
     2221 gacaaaatgc tggtgtatct tttcatacag ctagcaaacc agaataggtt atgctcgttt
     2281 tttgctttgt tttgtttttc aaaaagggaa gtaaacgaga accgttgact cctccattta
     2341 tggactcata cacagcagca ggagtgataa gcccacaagc tctctttccc gcctcgggaa
     2401 atctacacag ccaaaagcca cttagccata aatgacactt gtcagccttg aagcatcgga
     2461 gataactagc tgagtaaaat gatcctgttt tggaatttaa tgaaaaggtt aacagtaccc
     2521 aatgaaccca cccaagtgat gacatgggag gagcgaaacc gaaatctctt ttgctatata
     2581 aaggacacta ttttttaaaa aaaaataata aaaacagctc ccgctctctg tcctctctcc
     2641 ctcccttctc tccctcgcct ctctctcctc tctatattcc ctgttcttca ttgtgtacca
     2701 gtgtccgtga aagaccgcag taccacttac ctcagatgaa gcctgcgtgt tacatcctgt
     2761 aacacctttc attttgacat aagatggcta gccgaggtgc attatcttgg ttcggactgc
     2821 catctctgca ttcacgctgc acttttagcc agagatgcaa taatccccac tcctcaatac
     2881 tacctctgaa tgctacagtg aatttacagc cctgcacttg ttacacgctg ctagacacaa
     2941 gccctgcaag agaaaggaaa aaaaaaagcc caccaaaacc aaaccaaacc ttactgggtc
     3001 ggcatctcag ccatccccag ttctcgacca ttcttctctg tactcttact ccgtctcagc
     3061 aggctatgca tgtttctatg actttttttt tttttaatta aaatgttaca aatgcttgtg
     3121 gcagctttcc tgctagattg ttacattaat ttgaaacagt tttgagtcaa gttgctccta
     3181 ggttcttaag gagaattttt ttttcagtga cactactttg tatcacacac acacatctgt
     3241 agtgttcaaa tataagtctc caagtttgta cctcaaatga attattgaaa caaatggact
     3301 tcctgatttg caaggaacta cctccacact tccaaaggaa cgaacttgca gcctatatca
     3361 ctcattgatt tccttccccc atgtttgaag gagctcaaac ctcacctctc cctcattgaa
     3421 acattttttt tggtaaaaga cacttgatag aaacacaatt tttttacata cttttgcaaa
     3481 aataaatgaa ttaaaatcaa gccaaccttc aaagaaactt gaaattttgc tacaaccagc
     3541 tcagcctttt gcctaatgca atgaaaaagg aaaaaaatag atttctaaga tttgttgcct
     3601 agaagaatat gcttgaccga tattttttca tgtattttac acaatgtgat ttttgtaaaa
     3661 aaatgtctca agcagatttg ttttggacgc ttcttgtgta gagtttctat gcctttctct
     3721 cctattaagt gtgctgactt tccagagtgt tacccactgg gccaggaggt agtttctcat
     3781 agtggcttgt gtcagtataa gttaatactg aagccaaatg aaacaaacaa acaaccatgt
     3841 ctcttccagc tgttttcagg gaggttactt caaaggccac gtgccgctct gagactggca
     3901 gatggctcac tgttgtgagt cgccaaagga gctatggaga gattaaaatt caacatgact
     3961 gttaacaatg cattaaataa tcaaataaac agtggcttat aaatatcaga ttctcattcc
     4021 gggtcttcgg atgggcctta cagaaacctc attttggcca gctcataaaa actgaagcag
     4081 cttctcgtgt tggccagact cggcacaccg agcaatttcc atctctgatg aagttattcc
     4141 ttatttcctg tatgttgtac aatcaaaaca cactactacc tcttaagtcc cagtatacct
     4201 catttttcat actgaaaaaa aagaaaaaaa agagaaaaaa aaaggcttgt gtccaatgga
     4261 acagtgagaa catcataaaa tttttatata tatagtttat ttttgtggga gataaatttt
     4321 ataagactgt tttttactgt tgttggtcac agctaagtaa gactggacat ctaacttctc
     4381 taccatttct gcaagctaag tatgtttgca ggagaaactt atcaagacga ttaactgcat
     4441 ccaactttct ccccgttcct ttgtgtgccc tctgacttcg ttctctgttc cttgtgtaga
     4501 gtcgctgtct gtgattgtac ttggacggct tgcttgtggc aataccttct ctagtggatt
     4561 atcactgtct gcacaataaa cataacagcc aagggcgaat tctgcagata tccagcacag
     4621 tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
     4681 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4741 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4801 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     4861 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     4921 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4981 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5041 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5101 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5161 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5221 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5281 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5341 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     5401 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5461 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5521 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5581 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5641 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5701 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     5761 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     5821 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     5881 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     5941 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     6001 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6061 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6121 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6181 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6241 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     6301 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     6361 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     6421 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     6481 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     6541 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     6601 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     6661 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     6721 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     6781 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6841 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     6901 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     6961 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7021 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7081 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7141 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7201 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7321 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7501 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7621 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7681 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7741 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7801 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt
     7861 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7921 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7981 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8041 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8101 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8161 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8221 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8281 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8341 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8401 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8461 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8521 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8581 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8641 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8701 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8761 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8821 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8881 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8941 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9001 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9061 gccacctgac gtc
//
