LOCUS       sequence-81608-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of bacteriophage lambda CII for protein purification 
            (Intein-chiting binding domain fussion, New England Biolabs pTXB1 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  TITLE     Plamids for protein purification in E. coli of superfolder GFP, 
            CRP/CAP (E. coli), FIS (E. coli), CI (lambda phage) and CII (lambda 
            phage)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTXB1-CII
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..183)
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             complement(214..807)
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     terminator      826..860
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 agcagccgga tccccttcct gcagtcattg aagctgccac aaggcaggaa cgttggatgg
       61 ttcccatcct gccaaggagg tgtggggctg caaacattta tacgtcttgc cgttatatgt
      121 gaccaattgt cccgcagtat aagctgtgtt gacctgccaa gcggatacac caggatttgt
      181 cgtgaggcct gagttcagac cggtgaggcc agtagcgtgg ctgacgaacc cgttcgtgat
      241 aaacgcgtgg tctgccgtgt cgacacgaag gctatacacc ggctgcacgc cggcgtcggt
      301 gacactggcg actttcgcgt agtagaaccg cccgtcggtc agctcgtcgg cgatagcttg
      361 ggcgtccggg tctcggtggt gtgcttccaa gaaacgcacc agtccaggga cgccgactgt
      421 gtaggttgtg ggcgcaaatt cgggtttccc gcgggcaaaa cctgcacagt cgacgctgaa
      481 tgcgctgcgt tgaatcaccg cgtaatcgcc cggcttgatt tcgtcgatca gcttccacag
      541 cagggtcggc accccggcga cgtcgaccaa acacaacaac gggtggttcg cggtgcccgt
      601 cacacgcaga ccttcgaccg tacgcaccgt gtacaccgga tgctcgccgg agtggaacag
      661 ccggtcggcg agcacgggat tgccatgccg gtcaaggact ttcaggtcga tggcgttgtc
      721 actgttgggc cgcgcacccg gcacgatgtc ggcgatgcgt accgactcgc cctcgggtag
      781 ggcaactagt gcatctcccg tgatgcagaa ctccatctgg atttgttcag aacgctcggt
      841 tgccgccggg cgttttttat tggtgagaat cgcagcaact tgtcgcgcca atcgagccat
      901 gtcgtcgtca acgacc
//
