LOCUS       sequence-81614-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector, expresses Ngn2 and puromycin 
            resistent gene under control of TetON promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Zhang Y, Pak C, Han Y, Ahlenius H, Zhang Z, Chanda S, Marro S, 
            Patzke C, Acuna C, Covy J, Xu W, Yang N, Danko T, Chen L, Wernig M, 
            Sudhof TC
  TITLE     Rapid single-step induction of functional neurons from human 
            pluripotent stem cells.
  JOURNAL   Neuron. 2013 Jun 5;78(5):785-98. doi: 10.1016/j.neuron.2013.05.029.
  PUBMED    23764284
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.05"; journalName: "Neuron"; date: "2013-06-5-
            5"; volume: "78"; issue: "5"; pages: "785-98"
COMMENT     Sequence Label: pTet-O-Ngn2-puro
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      56..65
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             65..88
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccccg aattcgccac
       61 catggactac aaggacgacg atgacaagtt cgtcaaatct gagactctgg agttgaagga
      121 ggaagaggag gtactgatgc tgctgggctc ggcttccccg gcctcggcga ccctgacccc
      181 gatgtcctcc agcgcggacg aggaggagga cgaggagctg cgccggccgg gctccgcgcg
      241 tgggcagcgt ggagcggaag ccgggcaggg ggtgcagggc agtccggcgt cgggtgccgg
      301 gggttgccgg ccagggcggc tgctgggcct gatgcacgag tgcaagcgtc gcccgtcgcg
      361 ctcacgggcc gtctcccgag gtgccaagac ggcggagacg gtgcagcgca tcaagaagac
      421 ccgcaggctc aaggccaaca accgcgagcg caaccgcatg cacaacctaa acgccgcgct
      481 ggacgcgctg cgcgaggtgc tgcccacctt ccccgaggat gccaagctca cgaagatcga
      541 gacgctgcgc ttcgcccaca attacatctg ggcgctcacc gagactctgc gcctggcgga
      601 ccactgcgcc ggcgccggtg gcctccaggg ggcgctcttc acggaggcgg tgctcctgag
      661 cccgggagct gcgctcggcg ccagcgggga cagcccttct ccaccttcct cctggagctg
      721 caccaacagc ccggcgtcat cctccaactc cacgtcccca tacagctgca ctttatcgcc
      781 cgctagcccc gggtcagacg tggactactg gcagccccca cctccggaga agcatcgtta
      841 tgcgcctcac ctgcccctcg ccagggactg tatctcta
//
