LOCUS       sequence-81615-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Nd1 and puromycin resistent gene under control of TetON 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Zhang Y, Pak C, Han Y, Ahlenius H, Zhang Z, Chanda S, Marro S, 
            Patzke C, Acuna C, Covy J, Xu W, Yang N, Danko T, Chen L, Wernig M, 
            Sudhof TC
  TITLE     Rapid single-step induction of functional neurons from human 
            pluripotent stem cells.
  JOURNAL   Neuron. 2013 Jun 5;78(5):785-98. doi: 10.1016/j.neuron.2013.05.029.
  PUBMED    23764284
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.05"; journalName: "Neuron"; date: "2013-06-5-
            5"; volume: "78"; issue: "5"; pages: "785-98"
COMMENT     Sequence Label: pTet-O-Nd1-puro
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccccg aattcgctag
       61 ccaccatgac caaatcatac agcgagagcg ggctgatggg cgagcctcag ccccaaggtc
      121 ccccaagctg gacagatgag tgtctcagtt ctcaggacga ggaacacgag gcagacaaga
      181 aagaggacga gcttgaagcc atgaatgcag aggaggactc tctgagaaac gggggagagg
      241 aggaggagga agatgaggat ctagaggaag aggaggaaga agaagaggag gaggaggatc
      301 aaaagcccaa gagacggggt cccaaaaaga aaaagatgac caaggcgcgc ctagaacgtt
      361 ttaaattaag gcgcatgaag gccaacgccc gcgagcggaa ccgcatgcac gggctgaacg
      421 cggcgctgga caacctgcgc aaggtggtac cttgctactc caagacccag aaactgtcta
      481 aaatagagac actgcgcttg gccaagaact acatctgggc tctgtcagag atcctgcgct
      541 caggcaaaag ccctgatctg gtctccttcg tacagacgct ctgcaaaggt ttgtcccagc
      601 ccactaccaa tttggtcgcc ggctgcctgc agctcaaccc tcggactttc ttgcctgagc
      661 agaacccgga catgcccccg catctgccaa ccgccagcgc ttccttcccg gtgcatccct
      721 actcctacca gtcccctgga ctgcccagcc cgccctacgg caccatggac agctcccacg
      781 tcttccacgt caagccgccg ccacacgcct acagcgcagc tctggagccc ttctttgaaa
      841 gccccctaac tgactgcacc agcccttcct ttgacggacc cctcagcccg ccgctcagca
      901 tcaatggcaa cttctctttc aaacacgaac catccgccga gtttgaaaaa aattatgcct
      961 ttaccatgca ctaccctgca gcgacgctgg cagggc
//
