LOCUS       sequence-81616-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pre-2Ato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Kim J, Zhao T, Petralia RS, Yu Y, Peng H, Myers E, Magee JC
  TITLE     mGRASP enables mapping mammalian synaptic connectivity with light 
            microscopy.
  JOURNAL   Nat Methods. 2011 Dec 4;9(1):96-102. doi: 10.1038/nmeth.1784.
  PUBMED    22138823
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-4- 4"; volume: "9"; 
            issue: "1"; pages: "96-102"
COMMENT     Sequence Label: paavCAG-pre-mGRASP-2A-dTomato
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(755..808)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttactt atacagctca tccatgccat
       61 acaagaacag gtgatgccga ccttctgagc gttcgtactg ctcgacgatt gtgtagtcct
      121 cgttgtgact cgtgatgtcc aacttggtgt ccacgtagta gtagccaggc agctgaaccg
      181 gctttttggc catatatata gttttgaact caaccaggta gtgcccgcca tctttgagtt
      241 taagcgcctg atggatttcg cctttaagaa cgccatcgcg ggggtacagt cgttctgtag
      301 aggcctccca ccccatggtc ttcttctgca tcacggggcc atcgggggga aaatttgttc
      361 cgcgcatctt caccttgtaa atcagggtgc cgtcctgcag agaggagtct tgcgtgacag
      421 tgaccagccc accatcctca aaattcatta ctcgttccca cttaaatcct tcaggaaatg
      481 acagtttttt gtaatcaggt atatcagcag ggtgtttgac gtaagccttg ctaccgtaca
      541 tgaattgagg gctgaggatg tcccaggcga aaggaagggg gccacctttg gtaaccttga
      601 gcttggcggc ttgtgtgccc tcatatggtc ttccttcacc ctcaccttca atttcaaact
      661 cgtgcccgtt catgctaccc tccattctaa ctttgaatct cataaactcc ttaattactt
      721 cctcaccctt agacaccatt gtcgaccccg gggccggtcc gggattctcc tcgacgtcgc
      781 cacatgtgag cagagagccc cgaccctcag atcttacgta gtactcttta tccttg
//
