LOCUS       sequence-81620-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector, expresses Ngn2 and puromycin 
            resistent gene under control of TetON promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Zhang Y, Pak C, Han Y, Ahlenius H, Zhang Z, Chanda S, Marro S, 
            Patzke C, Acuna C, Covy J, Xu W, Yang N, Danko T, Chen L, Wernig M, 
            Sudhof TC
  TITLE     Rapid single-step induction of functional neurons from human 
            pluripotent stem cells.
  JOURNAL   Neuron. 2013 Jun 5;78(5):785-98. doi: 10.1016/j.neuron.2013.05.029.
  PUBMED    23764284
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.05"; journalName: "Neuron"; date: "2013-06-5-
            5"; volume: "78"; issue: "5"; pages: "785-98"
COMMENT     Sequence Label: pTet-O-Ngn2-puro
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..662)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(146..166)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     643..662
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(669..722)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattagc tagcttaatt
       61 aatcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg
      121 tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc
      181 tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg
      241 ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg
      301 ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg
      361 gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg
      421 ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg
      481 atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg
      541 cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg
      601 cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc
      661 atctcgagtg ggccgggatt ttcctccacg tccccgcatg ttagaagact tcccctgccc
      721 tctctagaga tacagtccct ggcgaggggc aggtgaggcg cataacgatg cttctccgga
      781 ggtgggggct gccagtagtc cacgtctgac ccgggg
//
