LOCUS       sequence-81625-e        1167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre recombinase expression plasmid for removal of floxed selectable 
            markers in the C. elegans germline.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Dickinson DJ, Ward JD, Reiner DJ, Goldstein B
  TITLE     Engineering the Caenorhabditis elegans genome using Cas9-triggered 
            homologous recombination.
  JOURNAL   Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641.
  PUBMED    23995389
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641."
COMMENT     Sequence Label: pDD104 (Peft-3::Cre)
FEATURES             Location/Qualifiers
     source          1..1167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    4..20
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9..31
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     28..44
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     28..44
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatggg tcacccaagg
       61 cgacaacgta agtgactgaa ggcattaaag gtacatgcgt ccgcgcaagt gtccgctttt
      121 gctggcctaa caagtaagag aaacaagtaa gaaagagtaa gagaaaactc acccgaggtg
      181 tctcttctag caaccgccta ttcagataat tttaagccag ttcgagagtt atttgtcgca
      241 tggtatagaa gctcgttatt tacattaaat ttttagtctt cccgcagaag tggacgatgc
      301 gatgggctac ttcaaaaatc tgacgccacg tgtcaaggac cttcacaaga gcatgacaaa
      361 cttggaagat attagcaagt ggcagaagaa ggccagtttc tctggcaccc ttgagaatta
      421 ctcgcgtctc ggtgacaaga tcaatgtgaa accatttatg gatgctgttg atgctagaat
      481 gggtgccgaa gctgatgccg tgaaaggggt ataaagctac tgttgcacga cgactcacag
      541 actaatcatt ccaggtcctc gcgatttgtg aaaaatacaa gtcattctac caaaacgagg
      601 gaaaacttca cgcggacagt atcgccaatt tgaataggac tcggctcgac atggacagtg
      661 cggcggataa atatgcgaac aacgagactg aagttaacaa gactcgtttg gataacagta
      721 ccacggaatt tgaagtggct tgtgagaggt ttgtaggtct ttcgtaggca cgatatcctt
      781 atttgaatgt gtttcagaat gcgagaactg gcgaacggaa tcaagacaat tgaatcgaac
      841 cattcttcct ggcaagacgg tcttatgaag gagattaaag tggcgttgcg taaataaaga
      901 aaggatcctc tttgaaaata aagattttct acattcactt atttttttag ctttatactt
      961 ttcagatcga atgaactgtt tccaacgttt ccatattaat tttttttagt taaaaaagtt
     1021 gggtattaac agttttgtta acagttttgt cttccgacag atgtgagacg gaaaaatggc
     1081 caaaattcga gattttgact aaaatcagtg tagctttttc aaaattttga accggcatga
     1141 aaaagtttga aaagttttcg agaagtt
//
