LOCUS       sequence-81627-e        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre recombinase expression plasmid for removal of floxed selectable 
            markers in the C. elegans germline.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Dickinson DJ, Ward JD, Reiner DJ, Goldstein B
  TITLE     Engineering the Caenorhabditis elegans genome using Cas9-triggered 
            homologous recombination.
  JOURNAL   Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641.
  PUBMED    23995389
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641."
COMMENT     Sequence Label: pDD104 (Peft-3::Cre)
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(185..209)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      214..537
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(541..561)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(569..586)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(569..585)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga
       61 gtttcaatac cggagatcat gcaagctggt ggctggacca atgtaaatat tgtcatgaac
      121 tatatccgta acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat
      181 tagtacccag ctttcttgta caaagtggga taaatgcaaa atcctttcaa gcattccctt
      241 cttctctatc actcttcttt ctttttgtca aaaaattctc tcgctaattt atttgctttt
      301 ttaatgttat tattttatga ctttttatag tcactgaaaa gtttgcatct gagtgaagtg
      361 aatgctatca aaatgtgatt ctgtctgatg tactttcaca atctctcttc aattccattt
      421 tgaagtgctt taaacccgaa aggttgagaa aaatgcgagc gctcaaatat ttgtattgtg
      481 ttcgttgagt gacccaacaa aaagaggaaa ctttattgtg ccgccaagaa aaaagtctca
      541 caactttatt atacatagtt gataattcac tggccgtcgt tttacactcg agacgtacgg
      601 tgcgcgcgat gcattcgaag atctgcccac tagtgagtcg tattataagt gcaagtaaga
      661 tcagtgtttg tatttaagac acacggccaa atttcttatt ttcctgctat tttctccaaa
      721 tatgattaaa atttgttcat ttcaaaattg cctccaactt gttttggggc aaaaatttca
      781 caaaacttca cagcaaaatg tgaaacaata ttgctatcta ttgaaaacat taatgatagt
      841 cgagtgactt cttgaaccaa tactcatcgc atcaaatgcc ttttctcgaa tactctgtaa
      901 taattggcat ataaaaacat acatactcat cagtttgaaa gctaaccttt accataatca
      961 ttactaaaat tggatttaaa gccggacacc aagaaaaaag atacggtaga taaactgaca
     1021 gatgagaagt ctcgatacga aataatggca tt
//
