LOCUS       sequence-81637-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Final destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, 
            Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  TITLE     Repeating pattern of non-RVD variations in DNA-binding modules 
            enhances TALEN activity.
  JOURNAL   Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
  PUBMED    24287550
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-29"; volume: "3:3379. doi"; pages: " 
            10.1038/srep03379"
COMMENT     Sequence Label: ptCMV-153/47-VR-NG
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    88..199
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     188..205
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(217..804)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
       61 atagaccgag atagggttga gtgttgtgat gcaatttcct cattttatta ggaaaggaca
      121 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
      181 tggcaactag aaggcacagt cgaggctgat tttttaaaag tttatctcgc cgttattaaa
      241 tttccttctc acttcctcaa gtgtcaatgt accagcttta atcatttctc caccaatcaa
      301 aagctcttct acactaagaa cagctccatt acaattagtg atatgattca atcttgtaag
      361 ctgagctttg tagtttcctt tgaaatgacc actcacaaac aagaacttaa attctgttac
      421 tgaagatgga tagactttcc accattcatt agggttgata tgcttgtttc ttgtttgatt
      481 ctcttcgaca tatctttgca tttcatctgc ttgaccaatt ggaagattat aacctcctga
      541 atatgcctta gtatcaacga tcacaccgta atcaatagga gatccgacag tataaattgc
      601 tccgtctggt ttccttgatc cacccaaatg tttaccacga taaccataaa ccttcataaa
      661 gaactccatc accttcattt caaggattct atcctgagtt gaatttcttg cgatttcaat
      721 caattcaata tattcatgtg gcacatattt caatttatgt ctaagttcag atttcttctc
      781 ttccaattca gatttcacta gctgggatct gatcaattcc ggcgcgtgcg gcaatccctt
      841 tttcactgca tccatggcag gacgtccgcc gaggcaggcc agcgctacaa gatgatcatt
      901 agtgagtgcg gccagcgcgg ggtccggcct cgaa
//
