LOCUS       sequence-81641-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PP1 alpha residues 7-330.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Kelker MS, Page R, Peti W
  TITLE     Crystal structures of protein phosphatase-1 bound to nodularin-R and
            tautomycin: a novel scaffold for structure-based drug design of 
            serine/threonine phosphatase inhibitors.
  JOURNAL   J Mol Biol. 2009 Jan 9. 385(1):11-21.
  PUBMED    18992256
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2009 Jan 9. 385(1):11-21."
COMMENT     Sequence Label: RP1B-PP1alpha
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      523..532
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa gctttcattt
       61 cttggctttg gcagagttgc ggggtggggt gatgggtcgg cctccagggt tcaggccact
      121 gaactgcccg tacttgccct tgttcttgtc ggccggctta aggatctgga aggagcacat
      181 gagggtctcg tccacgctca tcatggctcc ggcgttgtcg aactcgccac agtagttggg
      241 ggctgagaaa agtgtcacca gctgccgctt ggcaaagaac tcatagccgt cctccaccac
      301 ctggtgcgcc cggcagatga ggtccaggtc atgcttgtgc aggaacttgg ccaccacctc
      361 cgcgccgaag gtgaaggaga cgccgcggtc gttctcgccc cagccctgca cgtccttgtc
      421 ggggtcagac cacagcaggt cacacagcag gccctggtcg ggcacgtccg tgggccgcat
      481 gatgcgccgg atctgctcca tggactgcag gtcgggggag aggccgccat ggcagcagaa
      541 tatcttctcg tccacaatgg ccgcgatggg caggcagttg aagcagtcgg tgaacgtctt
      601 ccacagcttg atgttgtagc gtctcttgca ctcgtcgtag aagccgtaga tgcggttgat
      661 gctggcgcac tcgtggttcc cgcgcagcag gaagaagttc tcggggtact tgatcttgta
      721 ggccagcagc aggcagatgg tctccaggga ctgcttgccg cggtccacgt agtcacccag
      781 gaacaggtag ttgctctctg ggg
//
