LOCUS       sequence-81645-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Sakuma T, Hosoi S, Woltjen K, Suzuki KI, Kashiwagi K, Wada H, Ochiai
            H, Miyamoto T, Kawai N, Sasakura Y, Matsuura S, Okada Y, Kawahara A,
            Hayashi S, Yamamoto T
  TITLE     Efficient TALEN construction and evaluation methods for human cell 
            and animal applications.
  JOURNAL   Genes Cells. 2013 Feb 6. doi: 10.1111/gtc.12037.
  PUBMED    23388034
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Cells. 2013 Feb 6. doi: 10.1111/gtc.12037."
COMMENT     Sequence Label: pcDNA-TAL-NC2
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           82..124
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             204..224
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             234..257
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             258..284
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
     primer_bind     905..927
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     919..936
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     920..936
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        946..964
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt gcttgttctt tttgcagaag ctcagaataa acgctcaact
      121 ttggcagata agctcggatc taactcgaga aagatattgt atatatcgta acaataggag
      181 gttcaacaat ggcttcctcc cctccaaaga aaaagagaaa ggttgcggcc gctgactaca
      241 aggatgacga cgataaaagt tggaaggacg caagtggttg gtctagaatg catgcggccc
      301 cgcgacggcg tgctgcgcaa ccctccgacg cttcgccggc cgcgcaggtg gatctacgca
      361 cgctcggcta cagtcagcag cagcaagaga agatcaaacc gaaggtgcgt tcgacagtgg
      421 cgcagcacca cgaggcactg gtgggccatg ggtttacaca cgcgcacatc gttgcgctca
      481 gccaacaccc ggcagcgtta gggaccgtcg ctgtcacgta tcagcacata atcacggcgt
      541 tgccagaggc gacacacgaa gacatcgttg gcgtcggcaa acagtggtcc ggcgcacgcg
      601 ccctggaggc cttgctcacg gatgcggggg agttgagagg tccgccgtta cagttggaca
      661 caggccaact tgtgaagatt gcaaaacgtg gcggcgtgac cgcaatggag gcagtgcatg
      721 catcgcgcaa tgcactgacg ggtgcccccc tggagacggg cgccgctaca gggcgcgtcc
      781 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
      841 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
      901 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
      961 tagggcgaat
//
