LOCUS       sequence-81652-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for creating reversible targeting construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Chaiyachati BH, Kaundal RK, Zhao J, Wu J, Flavell R, Chi T
  TITLE     LoxP-FRT Trap (LOFT): a simple and flexible system for conventional 
            and reversible gene targeting.
  JOURNAL   BMC Biol. 2012 Nov 30;10:96. doi: 10.1186/1741-7007-10-96.
  PUBMED    23198860
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2012-11-30"; volume: "10:96. doi"; pages: " 
            10.1186/1741-7007-10-96"
COMMENT     Sequence Label: pTC201B
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..27
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11..27
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        48..66
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    98..131
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(160..207)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    join(221..286,287..324)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     primer_bind     complement(380..399)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac
       61 taaagggaac aaaagctgtc gagatccgat atcatcgata acttcgtata atgtatgcta
      121 tacgaagtta tcatatgcat gacgtctcaa aagcgctctg aagttcctat actttctaga
      181 gaataggaac ttcggaatag gaacttcaag atccacgcgt tagggcgcag tagtccaggg
      241 tttccttgat gatgtcatac ttatcctgtc cctttttttt ccacagctcg cggttgagga
      301 caaactcttc gcggtctttc cagtcgacat tgaacaagat ggattgcacg caggttctcc
      361 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
      421 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
      481 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
      541 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
      601 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
      661 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
      721 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
      781 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
      841 caggctcaag gcgcgcatgc cc
//
