LOCUS       sequence-81673-e         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and 
            Rbx1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, 
            Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, 
            White KP, Schulman BA
  TITLE     Structures of SPOP-substrate complexes: insights into molecular 
            architectures of BTB-Cul3 ubiquitin ligases.
  JOURNAL   Mol Cell. 2009 Oct 9;36(1):39-50. doi: 10.1016/j.molcel.2009.09.022.
  PUBMED    19818708
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2009.09"; journalName: "Mol Cell"; date: 
            "2009-10-9- 9"; volume: "36"; issue: "1"; pages: "39-50"
COMMENT     Sequence Label: Split 'n coexpress CUL3-RBX1
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             359..381
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atcgtcagtc agtcacgatg cggccgcagg cctctaatgc ccatacttct ggaactccca
       61 ctctctgttg tccaacggac acacctgcct cgttttgagc catcgagaga tgcagtggaa
      121 atgaaaagca tggttgcaga ctccccatgc aaccgtacac tcttcggaag tagctgacgc
      181 ctggttggcc tgacattcga tacaaagatc cataatgtgg ttcctgcaga tggcacagtt
      241 atcaaccaca atgtcccagg cccagagggc cactgcattc cactttttaa cttcaaagcg
      301 cttcttgccc gcgccgctgt tggtgccgct gggggtatcc acatccatat cgattatatc
      361 tccttcttaa agttaaacaa aattatttct gcggccgcat ggcggtacct tatgctacat
      421 atgtgtatac tttgcgatcc tcaggtgttc gtgccaaata ttctctctca ataagtcctt
      481 caatacgttt cttaataaca actggacttg gtaagaatcg cgccttcaac tgctgagtta
      541 cctccgctac tagaacattg tgctgcatct tctttctaga tttcattatc cgcactatag
      601 cagcttctat ctcatgtttt ctgtcgtcgt ctactttctg ccttgtttct ttcctctctg
      661 ggtcggattc accttgtttg gcagcaactg tttgaatctt gactctgtgt agtttggatg
      721 tgaattgatc attaactgta aa
//
