LOCUS       sequence-81678-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Sakuma T, Hosoi S, Woltjen K, Suzuki KI, Kashiwagi K, Wada H, Ochiai
            H, Miyamoto T, Kawai N, Sasakura Y, Matsuura S, Okada Y, Kawahara A,
            Hayashi S, Yamamoto T
  TITLE     Efficient TALEN construction and evaluation methods for human cell 
            and animal applications.
  JOURNAL   Genes Cells. 2013 Feb 6. doi: 10.1111/gtc.12037.
  PUBMED    23388034
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Cells. 2013 Feb 6. doi: 10.1111/gtc.12037."
COMMENT     Sequence Label: pcDNA-TAL-NC2
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(55..75)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(55..73)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(94..110)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(94..110)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(107..129)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    118..134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(142..148,149..166,167..172))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    187..208
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctatagggcg aattgggtac cgggcccccc ctcgaggtcc tccagctttt gttcccttta
       61 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
      121 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
      181 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccaccg
      241 gtcgtctcca acgaccacct cgtcgccttg gcctgcctcg gcggacgtcc tgccatggat
      301 gcagtgaaaa agggattgcc gcacgcgccg gaattgatca gatcccagct agtcaaaagt
      361 gaactggagg agaagaaatc tgaacttcgt cataaattga aatatgtgcc tcatgaatat
      421 attgaattaa ttgaaattgc cagaaattcc actcaggata gaattcttga aatgaaggta
      481 atggaatttt ttatgaaagt ttatggatat agaggtaaac atttgggtgg atcaaggaaa
      541 ccggacggag caatttatac tgtcggatct cctattgatt acggtgtgat cgtggatact
      601 aaagcttata gcggaggtta taatctgcca attggccaag cagatgaaat gcaacgatat
      661 gtcgaagaaa atcaaacacg aaacaaacat atcaacccta atgaatggtg gaaagtctat
      721 ccatcttctg taacggaatt taagttttta tttgtgagtg gtcactttaa a
//
