LOCUS       sequence-81685-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Sakuma T, Hosoi S, Woltjen K, Suzuki KI, Kashiwagi K, Wada H, Ochiai
            H, Miyamoto T, Kawai N, Sasakura Y, Matsuura S, Okada Y, Kawahara A,
            Hayashi S, Yamamoto T
  TITLE     Efficient TALEN construction and evaluation methods for human cell 
            and animal applications.
  JOURNAL   Genes Cells. 2013 Feb 6. doi: 10.1111/gtc.12037.
  PUBMED    23388034
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Cells. 2013 Feb 6. doi: 10.1111/gtc.12037."
COMMENT     Sequence Label: pFUS_A3B
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    158..179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(194..199,200..217,218..224)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    232..248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     237..259
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     256..272
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     256..272
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             268..645
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLDSH
                     HLVRGQAMVETLKTGRRFTTS"
     primer_bind     291..311
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        293..311
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(358..377)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(359..377)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(387..403)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(619..635)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      699..785
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtgcgtcacc cggcaacctt gggtagcagc gaagtcgagg catttctgtc ctggctggtc
       61 tagacgtctc gtcaggccat ggtcctggca cagcaccggc aacagccgct gcaccgtgag
      121 accaccggtg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      181 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      241 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
      301 ctcactaaag ggaacaaaag ctggaggacc tcgagggggg gcccggtacc caattcgccc
      361 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
      421 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      481 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      541 tgggacgcgc cctgtagcgg cgccggtctc gatagccacc acttggtccg gggtcaggcc
      601 atggtggaga cgcttaagac tggccgtcgt tttacaacgt cgtgactggg aaaacatcca
      661 tgctagcgtt aacgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
      721 cggaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
      781 caaatccgcc gggagcggat ttgaacgttg tgaagcaacg gcccgga
//
