LOCUS       sequence-81691-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the human MAVS CDS containing the point mutation M142A and
            an N-terminal HA tag ""strong translational context"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Brubaker SW, Gauthier AE, Mills EW, Ingolia NT, Kagan JC
  TITLE     A Bicistronic MAVS Transcript Highlights a Class of Truncated 
            Variants in Antiviral Immunity.
  JOURNAL   Cell. 2014 Feb 13;156(4):800-11. doi: 10.1016/j.cell.2014.01.021.
  PUBMED    24529381
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.01"; journalName: "Cell"; date: "2014-02-13- 
            13"; volume: "156"; issue: "4"; pages: "800-11"
COMMENT     Sequence Label: pcDNA3-HA-MAVS-M142A(KaganF61)
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             77..103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttacg atggactacc cctacgacgt ccccgactac gccggtaccg caccgtttgc
      121 tgaagacaag acctataagt atatctgccg caatttcagc aatttttgca atgtggatgt
      181 tgtagagatt ctgccttacc tgccctgcct cacagcaaga gaccaggatc gactgcgggc
      241 cacctgcaca ctctcaggga accgggacac cctctggcat ctcttcaata cccttcagcg
      301 gcggcccggc tgggtggagt acttcattgc ggcactgagg ggctgtgagc tagttgatct
      361 cgcggacgaa gtggcctctg tctacgagag ctaccagcct cggacctcgg accgtccccc
      421 agacccactg gagccaccgt cacttcctgc tgagaggcca gggcccccca cacctgctgc
      481 ggcccacagc atcccctaca acagctgcag agagaaggag ccaagttacc ccgcacctgt
      541 ccaggagacc caggcgccag agtccccagg agagaattca gagcaagccc tgcagacgct
      601 cagccccaga gccatcccaa ggaatccaga tggtggcccc ctggagtcct cctctgacct
      661 ggcagccctc agccctctga cctccagcgg gcatcaggag aaggacacag aactgggcag
      721 tacccacaca gcaggtgcga cctccagcct cacaccatcc cgtgggcctg tgtctccatc
      781 tgtctccttc cagcccctgg cccgttccac ccccagggca agccgcttgc ctggacccac
      841 agggtcagtt gtatctactg gcacctcctt ctcct
//
