LOCUS       sequence-8170-ex        3859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Alani E, Cao L, Kleckner N
  TITLE     A method for gene disruption that allows repeated use of URA3 
            selection in the construction of multiply disrupted yeast strains.
  JOURNAL   Genetics. 1987 Aug . 116(4):541-5.
  PUBMED    3305158
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            1987 Aug . 116(4):541-5."
COMMENT     Sequence Label: pNKY51
FEATURES             Location/Qualifiers
     source          1..3859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1198..1418
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             1419..2222
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 agatcttcca gtggtgcatg aacgcatgag aaagcccccg gaagatcatc ttccgggggc
       61 tttttttttg gcgcgcgata cagaccggtt cagacaggat aaagaggaac gcagaatgtt
      121 agacaacacc cgcttacgca tagctattca gaaatcaggc cgtttaagcg atgattcacg
      181 agaattgctg gcccgctgcg gcataaaaat taatttacac actcagcgcc tgattgcgat
      241 ggcggaaaac atgccgattg atatcctgcg cgtgcgtgat gatgacattc cgggtctggt
      301 aatggatggc gtggtcgatc tcggtattat cggcgaaaac gtgctggaag aagagctact
      361 caaccgccgc gcacagggcg aagatccacg ctatttaacc ctgcgccgtc ttgacttcgg
      421 cggctgccgt ttatcgctgg caacaccggt tgacgaagcc tgggacggcc cggccgcgct
      481 ggacggtaaa cgtatcgcta cctcatatcc gcacctcctc aaacgctacc tcgaccagaa
      541 aggcgtctct tttaaatcgt gtctgttaaa tggttctgtc gaagtcgcgc cgcgcgcggg
      601 gctggccgac gctatctgcg atttggtctc taccggcgcg acgcttgaag ctaacggcct
      661 gcgtgaagtc gaagttatct accgctctaa agcctgtctg attcagcgcg acggtgagat
      721 ggcacagagc aagcaagagc tgatcgataa attgctgacc cgtattcagg gcgtgattca
      781 ggcgcgcgaa tcgaaataca tcatgatgca cgcgccaagt gaacgcctgg aagaggttat
      841 cgccctgctg ccaggcgccg aaaggccgac aattctgccg ctggcaggcg agcaacagcg
      901 cgtggcgatg cacatggtca gcagcgaaac gttgttctgg gaaaccatgg agaaactgaa
      961 agcgcttggc gccagctcga ttctggtact gccgatcgag aagatgatgg agtgatctga
     1021 cgcctgatgg cgctgcgctt atcaggccta cgtaatgcgt tgatattttg ggttctgtag
     1081 gccggataag gcggaaccct gtgatggagt aaagaccatg agcttcaata ccctgattga
     1141 ctggaacagc ggatctgaat taattctcat gtttgacagc ttatcatcga taagcttttc
     1201 aattcaattc atcatttttt ttttattctt ttttttgatt tcggtttctt tgaaattttt
     1261 ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg
     1321 tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac
     1381 tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg
     1441 aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa
     1501 agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag
     1561 ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt
     1621 tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac
     1681 tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg
     1741 gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag
     1801 gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt
     1861 tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta
     1921 ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca
     1981 tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg
     2041 acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat
     2101 ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg
     2161 gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact
     2221 aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt
     2281 aattatatca gttattaccc gggaatctcg gtcgtaatga tttttataat gacgaaaaaa
     2341 aaaaaattgg aaagaaaaag ctttaatgcg gtagtttatc acagttaaat tgctaacgca
     2401 gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc
     2461 tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg
     2521 tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc
     2581 aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc
     2641 tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc
     2701 tgtggatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc atcttccggg
     2761 ggcttttttt ttggcgcgcg atacagaccg gttcagacag gataaagagg aacgcagaat
     2821 gttagacaac acccgcttac gcatagctat tcagaaatca ggccgtttaa gcgatgattc
     2881 acgagaattg ctggcccgct gcggcataaa aattaattta cacactcagc gcctgattgc
     2941 gatggcggaa aacatgccga ttgatatcct gcgcgtgcgt gatgatgaca ttccgggtct
     3001 ggtaatggat ggcgtggtcg atctcggtat tatcggcgaa aacgtgctgg aagaagagct
     3061 actcaaccgc cgcgcacagg gcgaagatcc acgctattta accctgcgcc gtcttgactt
     3121 cggcggctgc cgtttatcgc tggcaacacc ggttgacgaa gcctgggacg gcccggccgc
     3181 gctggacggt aaacgtatcg ctacctcata tccgcacctc ctcaaacgct acctcgacca
     3241 gaaaggcgtc tcttttaaat cgtgtctgtt aaatggttct gtcgaagtcg cgccgcgcgc
     3301 ggggctggcc gacgctatct gcgatttggt ctctaccggc gcgacgcttg aagctaacgg
     3361 cctgcgtgaa gtcgaagtta tctaccgctc taaagcctgt ctgattcagc gcgacggtga
     3421 gatggcacag agcaagcaag agctgatcga taaattgctg acccgtattc agggcgtgat
     3481 tcaggcgcgc gaatcgaaat acatcatgat gcacgcgcca agtgaacgcc tggaagaggt
     3541 tatcgccctg ctgccaggcg ccgaaaggcc gacaattctg ccgctggcag gcgagcaaca
     3601 gcgcgtggcg atgcacatgg tcagcagcga aacgttgttc tgggaaacca tggagaaact
     3661 gaaagcgctt ggcgccagct cgattctggt actgccgatc gagaagatga tggagtgatc
     3721 tgacgcctga tggcgctgcg cttatcaggc ctacgtaatg cgttgatatt ttgggttctg
     3781 taggccggat aaggcggaac cctgtgatgg agtaaagacc atgagcttca ataccctgat
     3841 tgactggaac agcggatcc
//
