LOCUS       sequence-81709-e        8971 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding constitutive expression of human ERAS 
            cDNA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8971)
  AUTHORS   Rais Y, Zviran A, Geula S, Gafni O, Chomsky E, Viukov S, Mansour AA,
            Caspi I, Krupalnik V, Zerbib M, Maza I, Mor N, Baran D, Weinberger 
            L, Jaitin DA, Lara-Astiaso D, Blecher-Gonen R, Shipony Z, Mukamel Z,
            Hagai T, Gilad S, Amann-Zalcenstein D, Tanay A, Amit I, Novershtern 
            N, Hanna JH
  TITLE     Deterministic direct reprogramming of somatic cells to pluripotency.
  JOURNAL   Nature. 2013 Oct 3;502(7469):65-70. doi: 10.1038/nature12587. Epub 
            2013 Sep 18.
  PUBMED    24048479
REFERENCE   2  (bases 1 to 8971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12587"; journalName: "Nature"; date: "2013-10-3- 3"; 
            volume: "502"; issue: "7469"; pages: "65-70"
COMMENT     Sequence Label: FUW-CAGGS-ERAS
FEATURES             Location/Qualifiers
     source          1..8971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    382..415
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             429..609
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(635..652)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    641..752
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(898..1486)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(978..997)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1657..2448,2449..2517))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2280..2299
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2518..2622)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2697..2716)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2888..3267
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3269..3467
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3422..3442
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3485..3665
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3712..3837
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4330..4563
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4748..4792
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5090..5207
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(5266..5284,5285..5307,5308..5326,5327..5349,5350..5368,
                     5369..5391,5392..5410,5411..5433,5434..5452,5453..5475,
                     5476..5494,5495..5517,5518..5536)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        5569..5606
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5603..5627
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     enhancer        5702..6081
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6083..6360
                     /label=chicken beta-actin promoter
     intron          6362..7379
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     7303..7325
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     7387..7406
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    8253..8841
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8306..8326)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8724..8735)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8844..8860)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8845..8861)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
       61 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
      121 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
      181 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
      241 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
      301 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
      361 tcacatggcc cgagagctgc aataacttcg tataatgtat gctatacgaa gttattccgg
      421 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      481 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      541 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      601 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
      661 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
      721 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
      781 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
      841 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      901 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      961 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1021 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1081 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1141 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1201 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1261 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1321 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1381 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1441 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1501 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1561 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1621 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     1681 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     1741 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     1801 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     1861 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     1921 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     1981 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     2041 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     2101 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     2161 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     2221 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     2281 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     2341 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     2401 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     2461 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     2521 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     2581 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     2641 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     2701 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     2761 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     2821 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     2881 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     2941 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     3001 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     3061 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     3121 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     3181 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     3241 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     3301 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     3361 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     3421 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg
     3481 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     3541 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     3601 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     3661 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     3721 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     3781 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     3841 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     3901 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     3961 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     4021 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     4081 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     4141 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     4201 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     4261 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     4321 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     4381 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     4441 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     4501 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     4561 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     4621 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     4681 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     4741 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     4801 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     4861 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     4921 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     4981 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     5041 ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa
     5101 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     5161 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     5221 gggacagcag agatccagtt tggttaatga tctcgagttt accactccct atcagtgata
     5281 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     5341 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     5401 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     5461 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     5521 ctatcagtga tagagaaaag tgaaagtcga gctcggtacc cgggtcgagt aggcgtgtac
     5581 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     5641 atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc cgcggccccg
     5701 agacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     5761 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     5821 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     5881 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     5941 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     6001 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     6061 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
     6121 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
     6181 atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
     6241 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
     6301 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
     6361 gggagtcgct gcgacgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
     6421 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     6481 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
     6541 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
     6601 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
     6661 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     6721 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     6781 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     6841 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     6901 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     6961 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     7021 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     7081 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     7141 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     7201 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     7261 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     7321 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     7381 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcatggagc
     7441 tgccaacaaa gcctggcacc ttcgacctgg gcctggccac atggagccct tccttccagg
     7501 gggaaaccca ccgggctcag gcacgccgca gggatgttgg caggcagctg cctgagtaca
     7561 aggctgtggt ggtgggcgcc agtggcgtgg gcaagagtgc gctgaccatc cagctgaacc
     7621 accagtgctt cgtggaggac cacgacccca ccatccagga ttcctactgg aaggagttga
     7681 ccctggacag tggggactgc attctgaatg tgctggacac agcagggcag gccatccata
     7741 gggccctgcg tgaccagtgc ctggctgtct gtgatggtgt gctgggcgtc ttcgctctcg
     7801 atgacccctc gtctctgatc cagctgcagc agatatgggc cacctggggc cctcaccccg
     7861 cccagcccct tgtcctcgtg ggcaacaagt gtgaccttgt gaccactgct ggagatgctc
     7921 atgccgctgc tgcagccctc gcacacagct ggggggccca cttcgtggag acctcggcca
     7981 aaacacggca aggcgtggag gaggcctttt ccctgctggt ccatgagatc cagagggtcc
     8041 aggaggccat ggcgaaggag cccatggcaa ggtcctgtag ggagaagacc cggcaccaga
     8101 aggccacctg ccactgtggc tgctctgtgg cctgagaatt cgccatggct ggacacctgg
     8161 cttcagactt cgccttctca cccccaccag gtgggggtga tgggtcagca gggctggagc
     8221 cgggctgggt gttcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     8281 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     8341 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     8401 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     8461 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     8521 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     8581 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     8641 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     8701 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     8761 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     8821 ccctttgggc cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag
     8881 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     8941 aggaggagga ggtgggtttt ccagtcacac c
//
