LOCUS       sequence-8171-ex       10445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10445)
  AUTHORS   Raser JM, O'Shea EK
  TITLE     Control of stochasticity in eukaryotic gene expression.
  JOURNAL   Science. 2004 Jun 18. 304(5678):1811-4.
  PUBMED    15166317
REFERENCE   2  (bases 1 to 10445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2004 Jun 18. 304(5678):1811-4."
COMMENT     Sequence Label: pJRL2 PHO5pr VYFP (EB#1547)
FEATURES             Location/Qualifiers
     source          1..10445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..44)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(24..42)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(63..79)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(63..79)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(76..98)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    87..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(111..117,118..135,136..141))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    156..177
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(294..311)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(465..1053)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(545..564)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1224..2015,2016..2084))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1847..1866
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2085..2189)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2215..2670)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2352..2373)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2564..2583
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2797..2819
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2811..2828
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2812..2828
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2838..2857
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2838..2856
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(3169..3885)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        6550..6770
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             6771..7574
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(8213..9028)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8213..9028)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8936..8955
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9020..9039
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gatctggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
       61 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      121 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      181 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
      241 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
      301 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      361 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      421 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      481 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      541 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      601 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
      661 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      721 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      781 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
      841 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      901 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
      961 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1021 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1081 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1141 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1201 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     1261 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     1321 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     1381 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     1441 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     1501 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     1561 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     1621 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     1681 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     1741 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     1801 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     1861 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     1921 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     1981 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     2041 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     2101 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     2161 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     2221 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     2281 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2341 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2401 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2461 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2521 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2581 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2641 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
     2701 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     2761 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     2821 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attggagctc tgatacacgg
     2881 tccaatggat aaacattttt tatcaacact atgatatata aatataatag tttttcgtat
     2941 atatattcct ttttttggtc aatttttgaa attttcgtag aaaagggaga gacaaaacac
     3001 attatatcaa tgaaaacgta caaaaagtag ataaagtcag tgcttaaaca cgtcttttcc
     3061 ttaaaaatac tttattattt ttattttatt gagagggtgg tttaaaaata gaaatagaga
     3121 gagaggtaca tacataaaca tacgcgcaca aaagcagaga gcggccgctt atttgtacaa
     3181 ttcatccata ccatgggtaa taccagcagc agtaacaaat tctaacaaga ccatgtggtc
     3241 tctcttttcg tttggatctt tggataaggc agattgatag gataagtaat ggttgtctgg
     3301 taacaagact ggaccatcac caattggagt attttgttga taatggtcag ctaattgaac
     3361 accaccatct tcaatgttgt gtctaatttt gaagttagct ttgataccat tcttttgttt
     3421 gtcagcagtg atgtaaacat tgtgagagtt atagttgtat tccaatttgt gacctaaaat
     3481 gttaccatct tctttaaaat caataccttt taattcgatt ctattaacta aggtatcacc
     3541 ttcaaacttg acttcagctc tggtcttgta gttaccgtca tctttgaaaa aaatagttct
     3601 ttcttgaaca taaccttctg gcatggcaga cttgaaaaag tcatgttgtt tcatatgatc
     3661 tgggtatcta gcaaaacaca ttaaaccata acctaaagta gtgactaagg ttggccatgg
     3721 aactggcaat ttaccagtag tacaaattaa ttttaaggtc aatttaccgt aagtagcatc
     3781 accttcacct tcaccggaga cagaaaattt gtgaccatta acatcaccat ctaattcaac
     3841 caaaattggg acaacaccag tgaataattc ttcaccttta gacattttgt tgaattctgg
     3901 taatctcgaa tttgcttgct ctatttgttg ttgttcttat tctcatgaga gatgaagcca
     3961 tactaacctc gacttagcaa aacatcagcg cttatatacg tttcatttcg acaattcaaa
     4021 gatgtttacc cttatcccaa atgggtatat gccttgccaa gtaaggtgac caatttgata
     4081 atttggcatg tgcgatctct tcgaaaacag ggaccagaat cataaattta gtctgtgcta
     4141 gtcccacgtg tgagtgccaa ggttgtatcg attcatttag agattgccta ttcaattaac
     4201 tcttttaatc acttttactt tttttggcat tgtgcagttg cgttctatgc gaaaacgtgc
     4261 taatttaata tatatttctt tgtgcagaca aagaaaaagc gctatgaacc ttttaccttc
     4321 gtttgaagtt tatagacgac gtccgcttac atgagaaaac attaaagcag cgcacataag
     4381 atgacttcca aatacgttga catatttgcg cattcttgtt gaatacaact ttcggatcct
     4441 tgtaatgccg gtctccggcg tcctgtaaag agagcgtgcg acacgccgct attagcgtgg
     4501 ggactgacat caggtcaaca ttattattgt agcgagctac tttcggtcag taaatagaac
     4561 atatgtagag atacaagcga ttattagaag gagcggacct acaacaaagt tgggtcacgt
     4621 ttctcgcaat tattacttgg atgccctcct agctaatgcc aatgtgcagt agtaacttat
     4681 cagtccgatg tgtaaaaaat agaactaatt ttttgcgaaa agatggacaa aaaccatctc
     4741 tggtcaattt tctcactaaa gagggacgga gtctcttgtt ttcttctatt agtgcacagg
     4801 gaatgttttg attttccctt tttttttctt tcgcttcttc aacagtggta aaaataaaaa
     4861 ttattggcga tgggataacc aaggaacatt gtatttagtc gacctcgaga tgctgtcgcc
     4921 gaagaagtta agaaaatcct tgcttaaaaa gattctcttt ttttatgata tttgtacata
     4981 aactttataa atgaaattca taatagaaac gacacgaaat tacaaaatgg aatatgttca
     5041 tagggtagac gaaactatat acgcaatcta catacattta tcaagaagga gaaaaaggag
     5101 gatgtaaagg aatacaggta agcaaattga taggcgcgcc ggtgagagcg ccggaaccgg
     5161 cttttcatat agaatagaga agcgttcatg actaaatgct tgcatcacaa tacttgaagt
     5221 tgacaatatt atttaaggac ctattgtttt ttccaatagg tggttagcaa tcgtcttact
     5281 ttctaacttt tcttaccttt tacatttcag caatatatat atatatattt caaggatata
     5341 ccattctaag atccgtcgat cttccagtgg tgcatgaacg catgagaaag cccccggaag
     5401 atcatcttcc gggggctttt tttttggcgc gcgatacaga ccggttcaga caggataaag
     5461 aggaacgcag aatgttagac aacacccgct tacgcatagc tattcagaaa tcaggccgtt
     5521 taagcgatga ttcacgagaa ttgctggccc gctgcggcat aaaaattaat ttacacactc
     5581 agcgcctgat tgcgatggcg gaaaacatgc cgattgatat cctgcgcgtg cgtgatgatg
     5641 acattccggg tctggtaatg gatggcgtgg tcgatctcgg tattatcggc gaaaacgtgc
     5701 tggaagaaga gctactcaac cgccgcgcac agggcgaaga tccacgctat ttaaccctgc
     5761 gccgtcttga cttcggcggc tgccgtttat cgctggcaac accggttgac gaagcctggg
     5821 acggcccggc cgcgctggac ggtaaacgta tcgctacctc atatccgcac ctcctcaaac
     5881 gctacctcga ccagaaaggc gtctctttta aatcgtgtct gttaaatggt tctgtcgaag
     5941 tcgcgccgcg cgcggggctg gccgacgcta tctgcgattt ggtctctacc ggcgcgacgc
     6001 ttgaagctaa cggcctgcgt gaagtcgaag ttatctaccg ctctaaagcc tgtctgattc
     6061 agcgcgacgg tgagatggca cagagcaagc aagagctgat cgataaattg ctgacccgta
     6121 ttcagggcgt gattcaggcg cgcgaatcga aatacatcat gatgcacgcg ccaagtgaac
     6181 gcctggaaga ggttatcgcc ctgctgccag gcgccgaaag gccgacaatt ctgccgctgg
     6241 caggcgagca acagcgcgtg gcgatgcaca tggtcagcag cgaaacgttg ttctgggaaa
     6301 ccatggagaa actgaaagcg cttggcgcca gctcgattct ggtactgccg atcgagaaga
     6361 tgatggagtg atctgacgcc tgatggcgct gcgcttatca ggcctacgta atgcgttgat
     6421 attttgggtt ctgtaggccg gataaggcgg aaccctgtga tggagtaaag accatgagct
     6481 tcaataccct gattgactgg aaggatctga attaattctc atgtttgaca gcttatcatc
     6541 gataagcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
     6601 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
     6661 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
     6721 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
     6781 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
     6841 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
     6901 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
     6961 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
     7021 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
     7081 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
     7141 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
     7201 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
     7261 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
     7321 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
     7381 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
     7441 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
     7501 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
     7561 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
     7621 gagcttcaat ttaattatat cagttattac ccgggaatct cggtcgtaat gatttttata
     7681 atgacgaaaa aaaaaaaatt ggaaagaaaa agctttaatg cggtagttta tcacagttaa
     7741 attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg
     7801 gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg ccgggcctct
     7861 tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg ctaggggggg
     7921 ggggggcgct gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca ggcctgaatc
     7981 gccccatcat ccagccagaa agtgagggag ccacggttga tgagagcttt gttgtaggtg
     8041 gaccagttgg tgattttgaa cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga
     8101 tgcgtgatct gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccgccgtccc
     8161 gtcaagtcag cgtaatgctc tgccagtgtt acaaccaatt aaccaattct gattagaaaa
     8221 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
     8281 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     8341 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     8401 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     8461 gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc cagccattac
     8521 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
     8581 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
     8641 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     8701 atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag
     8761 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
     8821 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     8881 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc
     8941 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgagc
     9001 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag
     9061 acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt
     9121 gagacacaac gtggctttcc cccccccccc ctagcgctat atgcgttgat gcaatttcta
     9181 tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct
     9241 tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatc
     9301 ttccagtggt gcatgaacgc atgagaaagc ccccggaaga tcatcttccg ggggcttttt
     9361 ttttggcgcg cgatacagac cggttcagac aggataaaga ggaacgcaga atgttagaca
     9421 acacccgctt acgcatagct attcagaaat caggccgttt aagcgatgat tcacgagaat
     9481 tgctggcccg ctgcggcata aaaattaatt tacacactca gcgcctgatt gcgatggcgg
     9541 aaaacatgcc gattgatatc ctgcgcgtgc gtgatgatga cattccgggt ctggtaatgg
     9601 atggcgtggt cgatctcggt attatcggcg aaaacgtgct ggaagaagag ctactcaacc
     9661 gccgcgcaca gggcgaagat ccacgctatt taaccctgcg ccgtcttgac ttcggcggct
     9721 gccgtttatc gctggcaaca ccggttgacg aagcctggga cggcccggcc gcgctggacg
     9781 gtaaacgtat cgctacctca tatccgcacc tcctcaaacg ctacctcgac cagaaaggcg
     9841 tctcttttaa atcgtgtctg ttaaatggtt ctgtcgaagt cgcgccgcgc gcggggctgg
     9901 ccgacgctat ctgcgatttg gtctctaccg gcgcgacgct tgaagctaac ggcctgcgtg
     9961 aagtcgaagt tatctaccgc tctaaagcct gtctgattca gcgcgacggt gagatggcac
    10021 agagcaagca agagctgatc gataaattgc tgacccgtat tcagggcgtg attcaggcgc
    10081 gcgaatcgaa atacatcatg atgcacgcgc caagtgaacg cctggaagag gttatcgccc
    10141 tgctgccagg cgccgaaagg ccgacaattc tgccgctggc aggcgagcaa cagcgcgtgg
    10201 cgatgcacat ggtcagcagc gaaacgttgt tctgggaaac catggagaaa ctgaaagcgc
    10261 ttggcgccag ctcgattctg gtactgccga tcgagaagat gatggagtga tctgacgcct
    10321 gatggcgctg cgcttatcag gcctacgtaa tgcgttgata ttttgggttc tgtaggccgg
    10381 ataaggcgga accctgtgat ggagtaaaga ccatgagctt caataccctg attgactgga
    10441 aggaa
//
