LOCUS       sequence-81713-e       12293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd generation lentiviral transfer vector driving expression of 
            human TGF-beta3 and RFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12293)
  AUTHORS   Brunger JM, Huynh NP, Guenther CM, Perez-Pinera P, Moutos FT, 
            Sanchez-Adams J, Gersbach CA, Guilak F
  TITLE     Scaffold-mediated lentiviral transduction for functional tissue 
            engineering of cartilage.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):E798-806. doi: 
            10.1073/pnas.1321744111. Epub 2014 Feb 18.
  PUBMED    24550481
REFERENCE   2  (bases 1 to 12293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1321744111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-03-4- 4"; volume: "111"; issue: "9"; pages: "E798-806"
COMMENT     Sequence Label: pLVE-hTGFB3-IRES-Red
FEATURES             Location/Qualifiers
     source          1..12293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2120..3297
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2351..3288
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3245..3265
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3345..3462
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4839..5406
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4857..5408
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5001..5018)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5228..5247
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5411..6088
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(5591..5611)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     6003..6026
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    6123..6711
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6176..6196)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6594..6605)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6855..6888)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6908..7088
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7113..7131)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7114..7131)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7421..7440)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7654..7673)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7773..7795
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7833..7851)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7919..8023
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8024..8092,8093..8884)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8242..8261)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9055..9643
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9544..9563
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9797..9814
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9884..9904)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9889..10218
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10069..10204
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11352..11417
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11547..11567
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11992..12126
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12029..12048)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12083..12102
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
     3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc
     3481 ctcgaggttt aaactacggg atccgtactt ttatctgtcc cctccacccc acagtggggc
     3541 aagcttgaat tctctagaaa tattctcgag gccgccacca tgaagatgca cttgcaaagg
     3601 gctctggtgg tcctggccct gctgaacttt gccacggtca gcctctctct gtccacttgc
     3661 accaccttgg acttcggcca catcaagaag aagagggtgg aagccattag gggacagatc
     3721 ttgagcaagc tcaggctcac cagcccccct gagccaacgg tgatgaccca cgtcccctat
     3781 caggtcctgg ccctttacaa cagcacccgg gagctgctgg aggagatgca tggggagagg
     3841 gaggaaggct gcacccagga aaacaccgag tcggaatact atgccaaaga aatccataaa
     3901 ttcgacatga tccaggggct ggcggagcac aacgaactgg ctgtctgccc taaaggaatt
     3961 acctccaagg ttttccgctt caatgtgtcc tcagtggaga aaaatagaac caacctattc
     4021 cgagcagaat tccgggtctt gcgggtgccc aaccccagct ctaagcggaa tgagcagagg
     4081 atcgagctct tccagatcct tcggccagat gagcacattg ccaaacagcg ctatatcggt
     4141 ggcaagaatc tgcccacacg gggcactgcc gagtggctgt cctttgatgt cactgacact
     4201 gtgcgtgagt ggctgttgag aagagagtcc aacttaggtc tagaaatcag cattcactgt
     4261 ccatgtcaca cctttcagcc caatggagat atcctggaaa acattcacga ggtgatggaa
     4321 atcaaattca aaggcgtgga caatgaggat gaccatggcc gtggagatct ggggcgcctc
     4381 aagaagcaga aggatcacca caaccctcat ctaatcctca tgatgattcc cccacaccgg
     4441 ctcgacaacc cgggccaggg gggtcagagg aagaagcggg ctttggacac caattactgc
     4501 ttccgcaact tggaggagaa ctgctgtgtg cgccccctct acattgactt ccgacaggat
     4561 ctgggctgga agtgggtcca tgaacctaag ggctactatg ccaacttctg ctcaggccct
     4621 tgcccatacc tccgcagtgc agacacaacc cacagcacgg tgctgggact gtacaacact
     4681 ctgaaccctg aagcatctgc ctcgccttgc tgcgtgcccc aggacctgga gcccctgacc
     4741 atcctgtact atgttgggag gacccccaaa gtggagcagc tctccaacat ggtggtgaag
     4801 tcttgtaaat gtagctgaac gcgtgtgccc gggtaaattc cgcccccccc cccctaacgt
     4861 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     4921 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     4981 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     5041 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     5101 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     5161 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     5221 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     5281 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     5341 gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     5401 tgataatacc atggcctcct ccgagaacgt catcaccgag ttcatgcgct tcaaggtgcg
     5461 catggagggc accgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     5521 ctacgagggc cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     5581 ctgggacatc ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc
     5641 cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     5701 gaacttcgag gacggcggcg tggcgaccgt gacccaggac tcctccctgc aggacggctg
     5761 cttcatctac aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtgatgca
     5821 gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
     5881 gaagggcgag acccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
     5941 caagtccatc tacatggcca agaagcccgt gcagctgccc ggctactact acgtggacgc
     6001 caagctggac atcacctccc acaacgagga ctacaccatc gtggagcagt acgagcgcac
     6061 cgagggccgc caccacctgt tcctgtagcg gccgcgactc tagaatttac tagtcatatg
     6121 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6181 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6241 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6301 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6361 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6421 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6481 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     6541 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     6601 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     6661 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcggtacg
     6721 tatggccagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     6781 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatgggatc
     6841 aattcaccat gggaataact tcgtatagca tacattatac gaagttatgc tgctttttgc
     6901 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6961 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7021 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7081 ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt
     7141 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct
     7201 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
     7261 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa
     7321 atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc
     7381 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     7441 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     7501 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     7561 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     7621 cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7681 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     7741 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7801 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     7861 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     7921 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7981 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     8041 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8101 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8161 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8221 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8281 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8341 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8401 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8461 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8521 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8581 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8641 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8701 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8761 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8821 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8881 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8941 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9001 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9061 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9121 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9181 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     9241 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9301 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9361 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9421 cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa
     9481 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9541 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9601 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9661 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9721 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     9781 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     9841 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta
     9901 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9961 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    10021 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
    10081 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
    10141 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    10201 ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat
    10261 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
    10321 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
    10381 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
    10441 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
    10501 atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt
    10561 gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag
    10621 cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat
    10681 gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac
    10741 tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat
    10801 atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca
    10861 atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc
    10921 aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa
    10981 cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg
    11041 ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc
    11101 tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga
    11161 aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt
    11221 atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct
    11281 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt
    11341 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
    11401 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg
    11461 cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta
    11521 ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg
    11581 actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg
    11641 cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg
    11701 aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt
    11761 ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta
    11821 cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga
    11881 ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
    11941 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
    12001 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    12061 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    12121 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
    12181 attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt cctctgaatt
    12241 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tag
//
