LOCUS       sequence-81715-e        9232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV39 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV39 L1L2
FEATURES             Location/Qualifiers
     source          1..9232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     regulatory      1582..1591
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3128..3249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3139..3158)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3193..3212
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3437..4025
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3926..3945
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4105..4408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4523..5828
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4865..5796
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     regulatory      5847..5856
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7275..7719
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7752..7798
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7790..7808
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7818..8612
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7818..8612
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7872..7891)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8482..8501
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8659..9231
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg gcaatgtgga gaagcagcga cagcatggtg
     1621 tacctgcccc ctcccagcgt ggccaaggtg gtcaacaccg acgactacgt gacccggacc
     1681 ggcatctact actacgccgg cagctctcgg ctgctgaccg tgggccaccc ctacttcaaa
     1741 gtgggcatga acggcggcag aaagcaggac atccccaagg tgtccgccta ccagtaccgg
     1801 gtgttcagag tgaccctgcc cgaccccaac aagttcagca tccccgacgc cagcctgtac
     1861 aaccccgaga cacagcggct ggtctgggcc tgcgtgggcg tggaagtggg cagaggccag
     1921 cccctgggcg tgggcatcag cggccacccc ctgtacaaca gacaggacga caccgagaac
     1981 agccccttca gcagcaccac caacaaggac agccgggaca acgtgtccgt ggactacaag
     2041 cagacccagc tgtgcatcat cggctgcgtg cctgccattg gcgagcactg gggcaagggc
     2101 aaggcctgca agcccaacaa tgtgtccacc ggcgactgcc cccctctgga actggtcaac
     2161 acacccatcg aggacggcga catgatcgac accggctacg gcgccatgga cttcggcgcc
     2221 ctgcaggaaa ccaagagcga ggtccccctg gacatctgcc agagcatctg caagtacccc
     2281 gactacctgc agatgagcgc cgacgtgtac ggcgactcca tgttcttttg cctgcggcgg
     2341 gagcagctgt tcgcccggca cttctggaac agaggcggca tggtcggcga cgctatccct
     2401 gcccagctgt atatcaaggg caccgacatc agagccaacc ccggcagctc cgtgtactgc
     2461 cccagcccca gcggctccat ggtcaccagc gacagccagc tgttcaacaa gccctactgg
     2521 ctgcacaagg cccagggcca caacaacggc atctgctggc acaaccagct gtttctgacc
     2581 gtggtggaca ccaccagaag caccaacttc accctgagca ccagcatcga gagcagcatc
     2641 cccagcacct acgacccctc caagttcaaa gagtacaccc ggcacgtcga ggaatacgac
     2701 ctgcagttca tcttccagct gtgtaccgtg accctgacca ccgacgtgat gagctacatc
     2761 cacaccatga acagcagcat cctggacaac tggaacttcg ccgtggcccc tccccctagc
     2821 gccagcctgg tggataccta cagatacctg cagagcgccg ccatcacctg ccagaaggac
     2881 gcccctgccc ccgagaagaa ggacccctac gacggcctga agttctggaa cgtggacctg
     2941 cgggagaagt tcagcctgga actcgaccag tttcccctgg gccggaagtt cctgctgcaa
     3001 gccagagtca gacggaggcc caccatcggc cccagaaagc ggcctgccgc tagcacctct
     3061 agcagctccg ccaccaagca caagcggaag cgggtgtcca agtgatagtc tagctggcca
     3121 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     3181 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     3241 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     3301 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggaaatgtt aattaactag
     3361 ccatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3421 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3481 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3541 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3601 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3661 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3721 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3781 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3841 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3901 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3961 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4021 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4081 acatgttctt aattaacctg caggcgttac ataacttacg gtaaatggcc cgcctggctg
     4141 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     4201 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     4261 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     4321 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     4381 ctacgtatta gtcatcgcta ttaccatgat gatgcggttt tggcagtaca tcaatgggcg
     4441 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     4501 tttgttttga ctagtggagc cgagagtaat tcatacaaaa ggagggatcg ccttcgcaag
     4561 gggagagccc agggaccgtc cctaaattct cacagaccca aatccctgta gccgccccac
     4621 gacagcgcga ggagcatgcg cccagggctg agcgcgggta gatcagagca cacaagctca
     4681 cagtccccgg cggtgggggg aggggcgcgc tgagcggggg ccagggagct ggcgcggggc
     4741 aaactgggaa agtggtgtcg tgtgctggct ccgccctctt cccgagggtg ggggagaacg
     4801 gtatataagt gcggtagtcg ccttggacgt tctttttcgc aacgggtttg ccgtcagaac
     4861 gcaggtgagt ggcgggtgtg gcttccgcgg gccccggagc tggagccctg ctctgagcgg
     4921 gccgggctga tatgcgagtg tcgtccgcag ggtttagctg tgagcattcc cacttcgagt
     4981 ggcgggcggt gcgggggtga gagtgcgagg cctagcggca accccgtagc ctcgcctcgt
     5041 gtccggcttg aggcctagcg tggtgtccgc cgccgcgtgc cactccggcc gcactatgcg
     5101 ttttttgtcc ttgctgccct cgattgcctt ccagcagcat gggctaacaa agggagggtg
     5161 tggggctcac tcttaaggag cccatgaagc ttacgttgga taggaatgga agggcaggag
     5221 gggcgactgg ggcccgcccg ccttcggagc acatgtccga cgccacctgg atggggcgag
     5281 gcctgtggct ttccgaagca atcgggcgtg agtttagcct acctgggcca tgtggcccta
     5341 gcactgggca cggtctggcc tggcggtgcc gcgttccctt gcctcccaac aagggtgagg
     5401 ccgtcccgcc cggcaccagt tgcttgcgcg gaaagatggc cgctcccggg gccctgttgc
     5461 aaggagctca aaatggagga cgcggcagcc cggtggagcg ggcgggtgag tcacccacac
     5521 aaaggaagag ggccttgccc ctcgccggcc gctgcttcct gtgaccccgt ggtctatcgg
     5581 ccgcatagtc acctcgggct tctcttgagc accgctcgtc gcggcggggg gaggggatct
     5641 aatggcgttg gagtttgttc acatttggtg ggtggagact agtcaggcca gcctggcgct
     5701 ggaagtcatt cttggaattt gcccctttga gtttggagcg aggctaattc tcaagcctct
     5761 tagcggttca aaggtatttt ctaaacccgt ttccaggtgt tgtgaaagcc accgctaatt
     5821 caaagcaatc cggagtatac ggatccgcca ccatggtgtc ccacagagcc gccagacgga
     5881 agcgggccag cgccaccgac ctgtatcgga cctgtaagca gagcggcacc tgcccccctg
     5941 atgtggtcga caaggtggag ggcaccacac tggccgacaa gatcctgcag tggaccagcc
     6001 tgggcatctt cctgggcggc ctgggcattg gcaccggcac aggcaccggc ggcagaaccg
     6061 gctacatccc cctcggcggc agacccaaca ccgtggtgga cgtgtccccc gccagacccc
     6121 ccgtggtcat cgagcccgtg ggccccagcg agcccagcat cgtgcagctg gtcgaggaca
     6181 gcagcgtgat caccagcggc acccccgtgc ccaccttcac cggcaccagc ggcttcgaga
     6241 ttacctctag ctccaccacc acccctgccg tgctggacat cacccccagc agcggcagcg
     6301 tgcagatcac ctccacctcc tacaccaacc ccgccttcac agacccaagc ctgatcgagg
     6361 tgccccagac cggcgagaca agcggcaaca tcttcgtgag cacccccacc tccggcacac
     6421 acggatacga ggaaatcccc atggaagtgt tcgccaccca cggcaccggg accgagccca
     6481 tcagcagcac ccctacccct ggcatctctc gggtggcagg acctcggctg tactctaggg
     6541 ctcaccagca ggtccgggtg tccaacttcg acttcgtgac ccaccccagc agcttcgtga
     6601 ccttcgacaa ccctgccttc gagcctgtgg acaccaccct gacctacgag gccgccgata
     6661 tcgcccccga ccccgacttc ctggacatcg tgcggctgca cagacccgcc ctgaccagcc
     6721 ggaagggcac cgtgcggttc tctcggctcg gcaagaaagc cacaatggtc accagacggg
     6781 gcacccagat cggcgcccag gtgcactact accacgacat cagctctatc gcccctgccg
     6841 agagcatcga gctgcagccc ctggtgcacg ccgagcccag cgacgcctcc gacgccctgt
     6901 tcgacatcta cgccgacgtg gacaacaaca cctacctgga caccgccttc aacaacaccc
     6961 gggacagcgg caccacctac aacaccggca gcctccccag cgtggccagc agcgccagca
     7021 ccaagtacgc caacaccacc atccctttca gcaccagctg gaacatgccc gtgaacaccg
     7081 gccctgatat cgctctgccc agcaccaccc cccagctgcc tctggtgccc agcggcccaa
     7141 tcgacacaac ctacgccatc accatccagg gcagcaacta ctacctgctg cccctgctgt
     7201 acttcttcct gaagaagcgg aagagaatcc cctacttctt cagcgacggc tacgtggccg
     7261 tgtgatagtc taggagcagg tttccccaat gacacaaaac gtgcaacttg aaactccgcc
     7321 tggtctttcc aggtctagag gggtaacact ttgtactgcg tttggctcca cgctcgatcc
     7381 actggcgagt gttagtaaca gcactgttgc ttcgtagcgg agcatgacgg ccgtgggaac
     7441 tcctccttgg taacaaggac ccacggggcc aaaagccacg cccacacggg cccgtcatgt
     7501 gtgcaacccc agcacggcga ctttactgcg aaacccactt taaagtgaca ttgaaactgg
     7561 tacccacaca ctggtgacag gctaaggatg cccttcaggt accccgaggt aacacgcgac
     7621 actcgggatc tgagaagggg actggggctt ctataaaagc gctcggttta aaaagcttct
     7681 atgcctgaat aggtgaccgg aggtcggcac ctttcctttg caattactga ccctatgaat
     7741 acactgactg tttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgactc
     7801 actataggag ggccaccatg attgaacaag atggattgca cgcaggttct ccggccgctt
     7861 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     7921 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     7981 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     8041 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     8101 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     8161 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     8221 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     8281 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     8341 aggcgagcat gcccgacggc gaggatctcg tcgtgacaca tggcgatgcc tgcttgccga
     8401 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     8461 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     8521 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     8581 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     8641 ccaagcgaat tcgctaggat tatccctaat acctgccacc ccactcttaa tcagtggtgg
     8701 aagaacggtc tcagaactgt ttgtttcaat tggccattta agtttagtag taaaagactg
     8761 gttaatgata acaatgcatc gtaaaacctt cagaaggaaa ggagaatgtt ttgtggacca
     8821 ctttggtttt cttttttgcg tgtggcagtt ttaagttatt agtttttaaa atcagtactt
     8881 tttaatggaa acaacttgac caaaaatttg tcacagaatt ttgagaccca ttaaaaaagt
     8941 taaatgagaa acctgtgtgt tcctttggtc aacaccgaga catttaggtg aaagacatct
     9001 aattctggtt ttacgaatct ggaaacttct tgaaaatgta attcttgagt taacacttct
     9061 gggtggagaa tagggttgtt ttccccccac ataattggaa ggggaaggaa tatcatttaa
     9121 agctatggga gggttgcttt gattacaaca ctggagagaa atgcagcatg ttgctgattg
     9181 cctgtcacta aaacaggcca aaaactgagt ccttgggttg catagaaagc tg
//
