LOCUS       sequence-81716-e        9226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV51 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9226)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV51 L1L2
FEATURES             Location/Qualifiers
     source          1..9226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     regulatory      1582..1591
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3125..3246
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3136..3155)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3190..3209
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3434..4022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3923..3942
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4102..4405
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4520..5825
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4862..5793
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     regulatory      5844..5853
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7269..7713
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7746..7792
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7784..7802
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7812..8606
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7812..8606
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7866..7885)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8476..8495
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8653..9225
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg gccctgtgga ggaccaacga cagcaaggtg
     1621 tacctgcccc ccgcccccgt gagcaggatc gtgaacaccg aggagtacat caccaggacc
     1681 ggcatctact actacgccgg cagcagcagg ctgatcaccc tgggccaccc ctacttcccc
     1741 ctgcccaaga ccagcaccag ggccgccatc cccaaggtga gcgccttcca gtacagggtg
     1801 ttcagggtgc agctccccga ccccaacaag ttcggcctgc ccgaccccaa cctgtacaac
     1861 cccgacaccg acaggctggt gtggggctgc gtgggcgtgg aggtgggcag gggccagccc
     1921 ctgggcgtgg gcctgagcgg ccaccccctg ttcaacaagt acgacgacac cgagaacagc
     1981 aggatcgcca acggcaacgc ccagcaggac gtgagggaca acaccagcgt ggacaacaag
     2041 cagacccagc tgtgcatcat cggctgcgcc ccccccatcg gcgagcactg gggcatcggc
     2101 accacctgca agaacacccc cgtgcccccc ggcgactgcc cccccctgga gctggtgagc
     2161 agcgtgatcc aggacggcga catgatcgac accggcttcg gcgccatgga cttcgccgcc
     2221 ctgcaggcca ccaagagcga cgtgcccctg gacatcagcc agagcgtgtg caagtacccc
     2281 gactacctga agatgagcgc cgacacctac ggcaacagca tgttcttcca cctgaggagg
     2341 gagcagatct tcgccaggca ctactacaac aagctgggca gcgtgggcga ggacatcccc
     2401 accgactact acatcaaggg cagcggcaac ggcagggacc ccatcgagag ctacatctac
     2461 agcgccaccc ccagcggcag catgatcacc agcgacagcc agatcttcaa caagccctac
     2521 tggctgcaca gggcccaggg ccacaacaac ggcatctgct ggaacaacca gctgttcatc
     2581 acctgcgtgg acaccaccag gagcaccaac ctgaccatca gcaccgccac cgccgccgtg
     2641 agccccacct tcacccccag caacttcaag cagtacatca ggcacggcga ggagtacgag
     2701 ctgcagttca tcttccagct gtgcaagatc accctgacca ccgaggtgat ggcctacctg
     2761 cacaccatgg accccaccat cctggagcag tggaacttcg gcctgaccct gccccccagc
     2821 gccagcctgg aggacgccta caggttcgtg aggaacgccg ccaccagctg ccagaaggac
     2881 accccccccc aggccaagcc cgaccccctg gccaagtaca agttctggga cgtggacctg
     2941 aaggagaggt tcagcctgga cctggaccag ttcgccctgg gcaggaagtt cctgctgcag
     3001 gtgggcgtgc agaggaagcc caggcccggc ctgaagaggc ccgctagcag cgccagctcc
     3061 agcagctcca gcagcgccaa gaggaagagg gtgaagaagt aatagtctag ctggccagac
     3121 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     3181 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     3241 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     3301 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg aaatgttaat taactagcca
     3361 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3421 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3481 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3541 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3601 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3661 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3721 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3781 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3841 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3901 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3961 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4021 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4081 tgttcttaat taacctgcag gcgttacata acttacggta aatggcccgc ctggctgacc
     4141 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     4201 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     4261 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     4321 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     4381 cgtattagtc atcgctatta ccatgatgat gcggttttgg cagtacatca atgggcgtgg
     4441 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     4501 gttttgacta gtggagccga gagtaattca tacaaaagga gggatcgcct tcgcaagggg
     4561 agagcccagg gaccgtccct aaattctcac agacccaaat ccctgtagcc gccccacgac
     4621 agcgcgagga gcatgcgccc agggctgagc gcgggtagat cagagcacac aagctcacag
     4681 tccccggcgg tggggggagg ggcgcgctga gcgggggcca gggagctggc gcggggcaaa
     4741 ctgggaaagt ggtgtcgtgt gctggctccg ccctcttccc gagggtgggg gagaacggta
     4801 tataagtgcg gtagtcgcct tggacgttct ttttcgcaac gggtttgccg tcagaacgca
     4861 ggtgagtggc gggtgtggct tccgcgggcc ccggagctgg agccctgctc tgagcgggcc
     4921 gggctgatat gcgagtgtcg tccgcagggt ttagctgtga gcattcccac ttcgagtggc
     4981 gggcggtgcg ggggtgagag tgcgaggcct agcggcaacc ccgtagcctc gcctcgtgtc
     5041 cggcttgagg cctagcgtgg tgtccgccgc cgcgtgccac tccggccgca ctatgcgttt
     5101 tttgtccttg ctgccctcga ttgccttcca gcagcatggg ctaacaaagg gagggtgtgg
     5161 ggctcactct taaggagccc atgaagctta cgttggatag gaatggaagg gcaggagggg
     5221 cgactggggc ccgcccgcct tcggagcaca tgtccgacgc cacctggatg gggcgaggcc
     5281 tgtggctttc cgaagcaatc gggcgtgagt ttagcctacc tgggccatgt ggccctagca
     5341 ctgggcacgg tctggcctgg cggtgccgcg ttcccttgcc tcccaacaag ggtgaggccg
     5401 tcccgcccgg caccagttgc ttgcgcggaa agatggccgc tcccggggcc ctgttgcaag
     5461 gagctcaaaa tggaggacgc ggcagcccgg tggagcgggc gggtgagtca cccacacaaa
     5521 ggaagagggc cttgcccctc gccggccgct gcttcctgtg accccgtggt ctatcggccg
     5581 catagtcacc tcgggcttct cttgagcacc gctcgtcgcg gcggggggag gggatctaat
     5641 ggcgttggag tttgttcaca tttggtgggt ggagactagt caggccagcc tggcgctgga
     5701 agtcattctt ggaatttgcc cctttgagtt tggagcgagg ctaattctca agcctcttag
     5761 cggttcaaag gtattttcta aacccgtttc caggtgttgt gaaagccacc gctaattcaa
     5821 agcaatccgg agtatacgga tccgccacca tggtcgccac cagggccagg cggaggaaga
     5881 gggccagcgt gacccagctg tacagcacct gcaaggccgc cggcacctgc ccccccgacg
     5941 tgatcaacaa ggtggagggc accaccctgg ccgacaagat cctgcagtgg agcgggctgg
     6001 gcatcttcct gggcggcctg ggcatcggca ccggcagcgg cagcggcggc aggaccggct
     6061 acatccccct gggcggcggc ggcaggcccg gcgtggtgga catcgctccc gccaggcccc
     6121 ccgtgatcat cgaacccatc gcccccaccg agccctccat cgtgaacctg gtggacgaca
     6181 gcagcatcat ccagagcggc agccccatcc ccaccttcac aggcaccgac ggcttcgaga
     6241 tcaccagcag cagcaccacc acccccgccg tgctggacat cacccccagc gccggcaccg
     6301 tgcacgtgag cagcaccaac atcgagaacc ccctgtacat cgagcccccc agcatcgagg
     6361 ccccccagag cggcgaggtg agcggccaca tcttcacctc caccctgacc agcggcaccc
     6421 acggctacga ggagatcccc atggaggtgt tcgccagcaa cgtgagcacc ggcaccgagc
     6481 ccatcagcag cacccccacc cccggcgtga gccggatcgc cgcccccagg ctgtacagca
     6541 agagctacac ccaggtgaag gtgaccaacc ccgacttcat cagcaagccc agcaccttcg
     6601 tgaccttcaa caaccccgcc ttcgagccca tcgacaccag catcaccttc gaggagccca
     6661 acgccgtggc ccccgacccc gacttcctgg acatcatcac cctgcacagg cccgccctga
     6721 ccagcaggag gggcaccgtg aggttcagca ggctgggcca gaaggccacc atgaggacca
     6781 ggagcggcaa gcagatcggc gccagggtgc actactacca cgacatcagc aggatcgccc
     6841 ccgccgacga gctggagatg cagcccctgc tgagccccag caacaactac agctacgaca
     6901 tctacgccga cctggacgag gccgagaccg gcttcatcca gcccacccac accaccccca
     6961 tgagccacag cagcgtgagc aggcagctgc ccagcctgag cagcagcatg agcagcagct
     7021 acgccaacgt gaccatcccc ttcagcaccc cctacagcgt gcccatccac accggccccg
     7081 acgtggtgct gcccaccagc cccaccgtgt ggccctacgt gccccacacc agcatcgaca
     7141 ccaagcacag catcgtgatc ctgggcggcg actactacct gtggccctac acccacctgc
     7201 tgaggaagag gaggaagagg attccctact tcttcaccga cggcatcgtg gcccactaat
     7261 agtctaggag caggtttccc caatgacaca aaacgtgcaa cttgaaactc cgcctggtct
     7321 ttccaggtct agaggggtaa cactttgtac tgcgtttggc tccacgctcg atccactggc
     7381 gagtgttagt aacagcactg ttgcttcgta gcggagcatg acggccgtgg gaactcctcc
     7441 ttggtaacaa ggacccacgg ggccaaaagc cacgcccaca cgggcccgtc atgtgtgcaa
     7501 ccccagcacg gcgactttac tgcgaaaccc actttaaagt gacattgaaa ctggtaccca
     7561 cacactggtg acaggctaag gatgcccttc aggtaccccg aggtaacacg cgacactcgg
     7621 gatctgagaa ggggactggg gcttctataa aagcgctcgg tttaaaaagc ttctatgcct
     7681 gaataggtga ccggaggtcg gcacctttcc tttgcaatta ctgaccctat gaatacactg
     7741 actgtttgac aattaatcat cggcatagta tatcggcata gtataatacg actcactata
     7801 ggagggccac catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     7861 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     7921 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     7981 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     8041 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     8101 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     8161 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     8221 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     8281 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     8341 gcatgcccga cggcgaggat ctcgtcgtga cacatggcga tgcctgcttg ccgaatatca
     8401 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     8461 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     8521 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     8581 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     8641 gaattcgcta ggattatccc taatacctgc caccccactc ttaatcagtg gtggaagaac
     8701 ggtctcagaa ctgtttgttt caattggcca tttaagttta gtagtaaaag actggttaat
     8761 gataacaatg catcgtaaaa ccttcagaag gaaaggagaa tgttttgtgg accactttgg
     8821 ttttcttttt tgcgtgtggc agttttaagt tattagtttt taaaatcagt actttttaat
     8881 ggaaacaact tgaccaaaaa tttgtcacag aattttgaga cccattaaaa aagttaaatg
     8941 agaaacctgt gtgttccttt ggtcaacacc gagacattta ggtgaaagac atctaattct
     9001 ggttttacga atctggaaac ttcttgaaaa tgtaattctt gagttaacac ttctgggtgg
     9061 agaatagggt tgttttcccc ccacataatt ggaaggggaa ggaatatcat ttaaagctat
     9121 gggagggttg ctttgattac aacactggag agaaatgcag catgttgctg attgcctgtc
     9181 actaaaacag gccaaaaact gagtccttgg gttgcataga aagctg
//
