LOCUS       sequence-81717-e        9213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV52 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV52 L1L2
FEATURES             Location/Qualifiers
     source          1..9213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     polyA_signal    3122..3243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3133..3152)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3187..3206
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3431..4019
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3920..3939
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4099..4402
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4517..5822
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4859..5790
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     misc_feature    7256..7700
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7733..7779
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7771..7789
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7799..8593
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7799..8593
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7853..7872)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8463..8482
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8640..9212
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg agcgtgtggc ggcccagcga ggccaccgtg
     1621 tacctgcccc ccgtgcccgt gtccaaggtg gtgtccaccg acgagtacgt gtcccggacc
     1681 agcatctact actacgccgg cagctctcgg ctcctgaccg tgggccaccc ctacttcagc
     1741 atcaagaata ccagctctgg caacggcaag aaagtgctgg tgcccaaggt gtccggcctg
     1801 cagtaccggg tgttccggat caagctgccc gaccccaaca agttcggctt ccccgacacc
     1861 agcttctaca accccgagac acagcggctg gtctgggcct gcaccggcct ggaaatcggc
     1921 agaggccagc ccctgggcgt gggcatctct ggccatcccc tgctgaacaa gttcgacgac
     1981 accgagacaa gcaacaagta cgccggcaag cccggcatcg acaaccggga gtgcctgagc
     2041 atggactaca agcagaccca gctgtgcatc ctgggctgca agccccccat cggcgagcac
     2101 tggggcaagg gcactccctg taacaacaac agcggcaacc ccggcgactg cccccctctg
     2161 cagctgatca acagcgtgat ccaggacggc gacatggtgg acaccggctt cggctgcatg
     2221 gacttcaaca ccctgcaggc cagcaagagc gacgtgccca tcgacatctg cagcagcgtg
     2281 tgcaagtacc ccgactacct gcagatggcc agcgagccct acggcgacag cctgttcttc
     2341 ttcctgcggc gggagcagat gttcgtgcgg cacttcttca acagagccgg caccctgggc
     2401 gaccccgtgc ctggcgacct gtacatccag ggcagcaact ccggcaacac cgccaccgtg
     2461 cagagcagcg ccttcttccc cacccccagc ggcagcatgg tcaccagcga gagccagctg
     2521 ttcaacaagc cctactggct gcagcgggcc cagggccaca acaacggcat ctgctggggc
     2581 aaccagctgt tcgtcaccgt ggtcgatacc actcggtcca ctaacatgac cctgtgcgcc
     2641 gaagtgaaga aagagagcac ctacaagaac gagaacttca aagagtacct gcggcacggc
     2701 gaggaattcg acctgcagtt catcttccag ctgtgcaaga tcaccctgac cgccgacgtg
     2761 atgacctaca tccacaagat ggacgccacc atcctggaag attggcagtt cggcctgacc
     2821 ccccctccta gcgccagcct ggaagatacc tacagattcg tgaccagcac cgccatcacc
     2881 tgccagaaga acaccccccc caagggcaaa gaggaccccc tgaaggacta catgttctgg
     2941 gaggtggacc tgaaagagaa gttcagcgcc gacctggacc agttccccct gggccggaag
     3001 ttcctgctgc aagccgggct gcaggccaga cccaagctga agaggcccgc cagcagcgcc
     3061 cccagaacca gcaccaagaa aaagaaagtg aagcgctgat agtctagctg gccagacatg
     3121 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     3181 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     3241 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     3301 ttttaaagca agtaaaacct ctacaaatgt ggtatggaaa tgttaattaa ctagccatga
     3361 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3421 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3481 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3541 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3601 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3661 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3721 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3781 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3841 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3901 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3961 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4021 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4081 tcttaattaa cctgcaggcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     4141 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     4201 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     4261 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     4321 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     4381 attagtcatc gctattacca tgatgatgcg gttttggcag tacatcaatg ggcgtggata
     4441 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     4501 ttgactagtg gagccgagag taattcatac aaaaggaggg atcgccttcg caaggggaga
     4561 gcccagggac cgtccctaaa ttctcacaga cccaaatccc tgtagccgcc ccacgacagc
     4621 gcgaggagca tgcgcccagg gctgagcgcg ggtagatcag agcacacaag ctcacagtcc
     4681 ccggcggtgg ggggaggggc gcgctgagcg ggggccaggg agctggcgcg gggcaaactg
     4741 ggaaagtggt gtcgtgtgct ggctccgccc tcttcccgag ggtgggggag aacggtatat
     4801 aagtgcggta gtcgccttgg acgttctttt tcgcaacggg tttgccgtca gaacgcaggt
     4861 gagtggcggg tgtggcttcc gcgggccccg gagctggagc cctgctctga gcgggccggg
     4921 ctgatatgcg agtgtcgtcc gcagggttta gctgtgagca ttcccacttc gagtggcggg
     4981 cggtgcgggg gtgagagtgc gaggcctagc ggcaaccccg tagcctcgcc tcgtgtccgg
     5041 cttgaggcct agcgtggtgt ccgccgccgc gtgccactcc ggccgcacta tgcgtttttt
     5101 gtccttgctg ccctcgattg ccttccagca gcatgggcta acaaagggag ggtgtggggc
     5161 tcactcttaa ggagcccatg aagcttacgt tggataggaa tggaagggca ggaggggcga
     5221 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgt
     5281 ggctttccga agcaatcggg cgtgagttta gcctacctgg gccatgtggc cctagcactg
     5341 ggcacggtct ggcctggcgg tgccgcgttc ccttgcctcc caacaagggt gaggccgtcc
     5401 cgcccggcac cagttgcttg cgcggaaaga tggccgctcc cggggccctg ttgcaaggag
     5461 ctcaaaatgg aggacgcggc agcccggtgg agcgggcggg tgagtcaccc acacaaagga
     5521 agagggcctt gcccctcgcc ggccgctgct tcctgtgacc ccgtggtcta tcggccgcat
     5581 agtcacctcg ggcttctctt gagcaccgct cgtcgcggcg gggggagggg atctaatggc
     5641 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     5701 cattcttgga atttgcccct ttgagtttgg agcgaggcta attctcaagc ctcttagcgg
     5761 ttcaaaggta ttttctaaac ccgtttccag gtgttgtgaa agccaccgct aattcaaagc
     5821 aatccggagt atacggtccg ccaccatgcg gtacaggcgg agcacccggc acaagcgggc
     5881 ctctgccacc cagctgtacc agacctgcaa ggccagcggc acctgccccc ctgacgtgat
     5941 ccccaaggtg gagggcacca caatcgccga ccagctgctg aagtacggca gcctgggcgt
     6001 gttcttcggc ggcctgggca tcggaacagg cgccggaagc ggcggcagag ccggctacgt
     6061 gcccctgagc accagacccc ccacctccag catcaccacc tccaccatcc ggcctcccgt
     6121 gaccgtggag cccatcggcc ccctggaacc cagcatcgtg tccatgatcg aggaaaccac
     6181 cttcatcgag agcggagccc cagcccctag catccctagc gccaccggct tcgacgtgac
     6241 cacctccgcc aacaacaccc ccgccatcat caacgtgacc agcatcggcg agagcagcgt
     6301 gcagagcgtg tccacccacc tgaaccccac cttcaccgag cccagcatca tccagccccc
     6361 tgcccctgcc gaagcctctg gccacgtgct gttcagcagc cccaccatca gcacccacac
     6421 ctacgaggaa atccccatgg acaccttcgt gacctccacc gacagctcta gcgtgactag
     6481 ctccaccccc atccccggct ctcggcctac cacccggctg ggcctgtaca gccgggccac
     6541 ccagcaggtc aaggtggtgg accccgcctt catgagcagc ccccagaaac tggtcaccta
     6601 caacaacccc gtgttcgagg gcgtggacac cgacgagaca atcatcttcg acagaagcca
     6661 gctgctgcct gcccccgacc ccgacttcct ggacatcatt gccctgcaca gacccgccct
     6721 gaccagcaga cggggcaccg tgcggttctc tcggctgggc aacaaggcca ccctgcggac
     6781 cagaagcggc aagcagatcg gcgccagagt gcactactac cacgacatca gccccatcca
     6841 gcccgccgag gtgcaggaag atatcgagct gcagcccctg ctgccccagt ccgtgagccc
     6901 ctacaccatc aacgacggcc tgtacgacgt gtacgccgac agcctgcagc agcccacctt
     6961 ccacctgccc tccaccctga gcacacacaa caataccttc accgtgccta tcaacagcgg
     7021 catcgacttc gtgtaccagc ccaccatgag catcgagtcc ggccctgaca tccccctgcc
     7081 cagcctgccc acccacaccc ccttcgtgcc tatcgccccc accgccccca gcacctccat
     7141 catcgtggac ggcaccgact tcatcctgca ccccagctac tttctgctgc ggcggaggcg
     7201 gaagcggttc ccctacttct tcaccgacgt gcgggtggcc gcctgatagt ctaggagcag
     7261 gtttccccaa tgacacaaaa cgtgcaactt gaaactccgc ctggtctttc caggtctaga
     7321 ggggtaacac tttgtactgc gtttggctcc acgctcgatc cactggcgag tgttagtaac
     7381 agcactgttg cttcgtagcg gagcatgacg gccgtgggaa ctcctccttg gtaacaagga
     7441 cccacggggc caaaagccac gcccacacgg gcccgtcatg tgtgcaaccc cagcacggcg
     7501 actttactgc gaaacccact ttaaagtgac attgaaactg gtacccacac actggtgaca
     7561 ggctaaggat gcccttcagg taccccgagg taacacgcga cactcgggat ctgagaaggg
     7621 gactggggct tctataaaag cgctcggttt aaaaagcttc tatgcctgaa taggtgaccg
     7681 gaggtcggca cctttccttt gcaattactg accctatgaa tacactgact gtttgacaat
     7741 taatcatcgg catagtatat cggcatagta taatacgact cactatagga gggccaccat
     7801 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     7861 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     7921 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     7981 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     8041 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     8101 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     8161 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     8221 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     8281 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     8341 cgaggatctc gtcgtgacac atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     8401 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     8461 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     8521 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     8581 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgaa ttcgctagga
     8641 ttatccctaa tacctgccac cccactctta atcagtggtg gaagaacggt ctcagaactg
     8701 tttgtttcaa ttggccattt aagtttagta gtaaaagact ggttaatgat aacaatgcat
     8761 cgtaaaacct tcagaaggaa aggagaatgt tttgtggacc actttggttt tcttttttgc
     8821 gtgtggcagt tttaagttat tagtttttaa aatcagtact ttttaatgga aacaacttga
     8881 ccaaaaattt gtcacagaat tttgagaccc attaaaaaag ttaaatgaga aacctgtgtg
     8941 ttcctttggt caacaccgag acatttaggt gaaagacatc taattctggt tttacgaatc
     9001 tggaaacttc ttgaaaatgt aattcttgag ttaacacttc tgggtggaga atagggttgt
     9061 tttcccccca cataattgga aggggaagga atatcattta aagctatggg agggttgctt
     9121 tgattacaac actggagaga aatgcagcat gttgctgatt gcctgtcact aaaacaggcc
     9181 aaaaactgag tccttgggtt gcatagaaag ctg
//
