LOCUS       sequence-81718-e        9196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV56 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV56 L1L2
FEATURES             Location/Qualifiers
     source          1..9196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     regulatory      1582..1591
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3110..3231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3121..3140)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3175..3194
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3419..4007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3908..3927
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4087..4390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4505..5810
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4847..5778
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     regulatory      5829..5838
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7239..7683
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7716..7762
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7754..7772
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7782..8576
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7782..8576
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7836..7855)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8446..8465
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8623..9195
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg gccacctggc ggcccagcga gaacaaggtg
     1621 tacctgcccc ccacccccgt gtccaaggtg gtggccaccg acagctacgt gaagcggacc
     1681 agcatcttct accacgccgg cagcagcaga ctgctggccg tgggccaccc ctactacagc
     1741 gtgaccaagg acaacaccaa gaccaacatc cccaaggtgt ccgcctacca gtaccgggtg
     1801 ttcagagtgc ggctgcctga ccctaacaag ttcggcctgc ccgacaccaa tatctacaac
     1861 cccgaccagg aacggctggt ctgggcctgc gtgggcctgg aagtgggcag aggccagcct
     1921 ctgggagctg gcctgagcgg ccaccccctg ttcaaccggc tggacgacac cgagagcagc
     1981 aacctggcca acaacaacgt gatcgaggac agccgggaca acatcagcgt ggacggcaag
     2041 cagacccagc tgtgcatcgt gggctgcaca cccgccatgg gcgagcactg gaccaagggc
     2101 gccgtgtgca agagcaccca ggtcaccacc ggcgactgcc cccctctggc cctgatcaac
     2161 acccccatcg aggacggcga catgatcgac accggcttcg gcgccatgga cttcaaggtg
     2221 ctgcaggaaa gcaaggccga ggtccccctg gacatcgtgc agagcacctg caagtacccc
     2281 gactacctga agatgagcgc cgacgcctac ggcgacagca tgtggttcta cctgcggcgg
     2341 gagcagctgt tcgcccggca ctacttcaac agagccggca aagtgggcga gacaatcccc
     2401 gccgagctgt acctgaaggg cagcaacgga cgggagcctc ctcccagcag cgtgtacgtg
     2461 gccaccccca gcggcagcat gatcaccagc gaggcccagc tgttcaacaa gccctactgg
     2521 ctgcagcggg cccagggcca caacaacggc atctgctggg gcaaccagct gttcgtgacc
     2581 gtcgtggaca ctacccggtc caccaacatg accatcagca ccgccaccga gcagctgtcc
     2641 aagtacgacg cccggaagat caaccagtac ctgcggcacg tggaggaata tgagctgcag
     2701 ttcgtctttc agctgtgcaa gatcaccctg agcgccgaag tgatggccta cctgcacaac
     2761 atgaacgcca acctgctgga agattggaac atcggcctga gcccccctgt ggctacctct
     2821 ctggaagata agtacagata cgtgcggagc accgccatca cctgccagag agagcagccc
     2881 cccaccgaga agcaggaccc cctggccaag tacaagttct gggacgtgaa cctgcaggac
     2941 agcttcagca ccgacctgga ccagttcccc ctgggccgga agttcctgat gcagctgggc
     3001 accagatcca agcccgccgt ggccacaagc aagaagcgga gcgcccccac aagcaccagc
     3061 acccccgcca agcggaagcg gagatgatag tctagctggc cagacatgat aagatacatt
     3121 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     3181 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     3241 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     3301 taaaacctct acaaatgtgg tatggaaatg ttaattaact agccatgacc aaaatccctt
     3361 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3421 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3481 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3541 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3601 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3661 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3721 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3781 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3841 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3901 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3961 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4021 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc ttaattaacc
     4081 tgcaggcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     4141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     4201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     4261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     4321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     4381 tattaccatg atgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     4441 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt gactagtgga
     4501 gccgagagta attcatacaa aaggagggat cgccttcgca aggggagagc ccagggaccg
     4561 tccctaaatt ctcacagacc caaatccctg tagccgcccc acgacagcgc gaggagcatg
     4621 cgcccagggc tgagcgcggg tagatcagag cacacaagct cacagtcccc ggcggtgggg
     4681 ggaggggcgc gctgagcggg ggccagggag ctggcgcggg gcaaactggg aaagtggtgt
     4741 cgtgtgctgg ctccgccctc ttcccgaggg tgggggagaa cggtatataa gtgcggtagt
     4801 cgccttggac gttctttttc gcaacgggtt tgccgtcaga acgcaggtga gtggcgggtg
     4861 tggcttccgc gggccccgga gctggagccc tgctctgagc gggccgggct gatatgcgag
     4921 tgtcgtccgc agggtttagc tgtgagcatt cccacttcga gtggcgggcg gtgcgggggt
     4981 gagagtgcga ggcctagcgg caaccccgta gcctcgcctc gtgtccggct tgaggcctag
     5041 cgtggtgtcc gccgccgcgt gccactccgg ccgcactatg cgttttttgt ccttgctgcc
     5101 ctcgattgcc ttccagcagc atgggctaac aaagggaggg tgtggggctc actcttaagg
     5161 agcccatgaa gcttacgttg gataggaatg gaagggcagg aggggcgact ggggcccgcc
     5221 cgccttcgga gcacatgtcc gacgccacct ggatggggcg aggcctgtgg ctttccgaag
     5281 caatcgggcg tgagtttagc ctacctgggc catgtggccc tagcactggg cacggtctgg
     5341 cctggcggtg ccgcgttccc ttgcctccca acaagggtga ggccgtcccg cccggcacca
     5401 gttgcttgcg cggaaagatg gccgctcccg gggccctgtt gcaaggagct caaaatggag
     5461 gacgcggcag cccggtggag cgggcgggtg agtcacccac acaaaggaag agggccttgc
     5521 ccctcgccgg ccgctgcttc ctgtgacccc gtggtctatc ggccgcatag tcacctcggg
     5581 cttctcttga gcaccgctcg tcgcggcggg gggaggggat ctaatggcgt tggagtttgt
     5641 tcacatttgg tgggtggaga ctagtcaggc cagcctggcg ctggaagtca ttcttggaat
     5701 ttgccccttt gagtttggag cgaggctaat tctcaagcct cttagcggtt caaaggtatt
     5761 ttctaaaccc gtttccaggt gttgtgaaag ccaccgctaa ttcaaagcaa tccggagtat
     5821 acggatccgc caccatggtc gcccaccggg ccaccagacg gaagcgggcc agcgccaccc
     5881 agctgtacaa gacctgcaag ctgtccggca cctgccccga ggacgtggtc aacaagatcg
     5941 agcagaaaac ctgggccgac aagatcctgc agtggggcag cctgttcacc tacttcggcg
     6001 gcctgggcat cggcaccggc acaggctctg gcggaagagc cggctacgtg cccctgggca
     6061 gccggcccag caccatcgtg gacgtgaccc ccgccagacc ccccatcgtg gtggagagcg
     6121 tgggccccac cgaccccagc atcgtgaccc tggtggagga aagcagcgtg atcgagagcg
     6181 gagccggcat ccccaacttc accggcagcg gcggcttcga gatcaccagc agctccacca
     6241 ccacccctgc cgtgctggac atcaccccca cctccagcac cgtgcacgtg tccagcaccc
     6301 acatcaccaa ccccctgttc atcgaccccc ccgtgatcga ggccccccag actggcgagg
     6361 tgtcaggcaa catcctgatc agcaccccta ccagcggcat ccacagctac gaggaaatcc
     6421 ccatgcagac cttcgccgtg catggcagcg gcaccgagcc catcagcagc acccccatcc
     6481 ccggcttcag acggattgcc gcccctcggc tgtaccggaa ggcattccag caggtcaaag
     6541 tgaccgaccc cgccttcctc gacagacctg ccaccctggt gtccgccgac aaccctctgt
     6601 tcgagggcac cgacaccagc ctggccttca gccccagcgg cgtggccccc gatcccgact
     6661 tcatgaatat cgtggccctg cacaggcccg ccttcaccac ccgcagaggc ggcgtgcggt
     6721 tcagccggct gggccggaag gccaccatcc agaccagacg gggcacccag atcggcgcca
     6781 gagtgcacta ctactacgac atcagcccta tcgcccaggc cgaggaaatc gagatgcagc
     6841 ccctgctgtc cgccaataac agcttcgacg gcctgtacga catctacgcc aacatcgacg
     6901 acgaggcccc tggcctgagc agccagagcg tggccacccc cagcgcccac ctccccatta
     6961 agcccagcac cctgagcttc gccagcaaca ccaccaacgt gaccgcccct ctgggcaacg
     7021 tgtgggagac acctttctac agcggccctg acatcgtcct gcccactggc cctagcacct
     7081 ggcccttcgt gccccagagc ccctacgacg tgacccacga cgtgtacatc cagggctcta
     7141 gcttcgccct gtggcccgtg tacttcttcc ggcggaggcg gcggaagaga atcccctact
     7201 tcttcgctga cggcgatgtg gctgcctgat agtctaggag caggtttccc caatgacaca
     7261 aaacgtgcaa cttgaaactc cgcctggtct ttccaggtct agaggggtaa cactttgtac
     7321 tgcgtttggc tccacgctcg atccactggc gagtgttagt aacagcactg ttgcttcgta
     7381 gcggagcatg acggccgtgg gaactcctcc ttggtaacaa ggacccacgg ggccaaaagc
     7441 cacgcccaca cgggcccgtc atgtgtgcaa ccccagcacg gcgactttac tgcgaaaccc
     7501 actttaaagt gacattgaaa ctggtaccca cacactggtg acaggctaag gatgcccttc
     7561 aggtaccccg aggtaacacg cgacactcgg gatctgagaa ggggactggg gcttctataa
     7621 aagcgctcgg tttaaaaagc ttctatgcct gaataggtga ccggaggtcg gcacctttcc
     7681 tttgcaatta ctgaccctat gaatacactg actgtttgac aattaatcat cggcatagta
     7741 tatcggcata gtataatacg actcactata ggagggccac catgattgaa caagatggat
     7801 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     7861 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     7921 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     7981 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     8041 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     8101 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     8161 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     8221 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     8281 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     8341 cacatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     8401 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     8461 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     8521 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     8581 gactctgggg ttcgaaatga ccgaccaagc gaattcgcta ggattatccc taatacctgc
     8641 caccccactc ttaatcagtg gtggaagaac ggtctcagaa ctgtttgttt caattggcca
     8701 tttaagttta gtagtaaaag actggttaat gataacaatg catcgtaaaa ccttcagaag
     8761 gaaaggagaa tgttttgtgg accactttgg ttttcttttt tgcgtgtggc agttttaagt
     8821 tattagtttt taaaatcagt actttttaat ggaaacaact tgaccaaaaa tttgtcacag
     8881 aattttgaga cccattaaaa aagttaaatg agaaacctgt gtgttccttt ggtcaacacc
     8941 gagacattta ggtgaaagac atctaattct ggttttacga atctggaaac ttcttgaaaa
     9001 tgtaattctt gagttaacac ttctgggtgg agaatagggt tgttttcccc ccacataatt
     9061 ggaaggggaa ggaatatcat ttaaagctat gggagggttg ctttgattac aacactggag
     9121 agaaatgcag catgttgctg attgcctgtc actaaaacag gccaaaaact gagtccttgg
     9181 gttgcataga aagctg
//
