LOCUS       sequence-81719-e        9223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV59 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV59 L1L2
FEATURES             Location/Qualifiers
     source          1..9223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     regulatory      1582..1591
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3137..3258
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3148..3167)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3202..3221
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3446..4034
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3935..3954
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4114..4417
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4532..5837
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4874..5805
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     regulatory      5856..5865
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7266..7710
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7743..7789
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7781..7799
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7809..8603
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7809..8603
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7863..7882)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8473..8492
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8650..9222
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg gcactgtgga gaagcagcga caacaaggtg
     1621 tacctgcccc ctcccagcgt ggccaaggtg gtgtccaccg acgagtacgt gacccggacc
     1681 agcatcttct accacgccgg cagctctcgg ctgctgaccg tgggccaccc ctacttcaag
     1741 gtgcccaagg gcggcaacgg ccggcaggac gtgcccaagg tgtccgccta ccagtaccgg
     1801 gtgttcagag tgaagctgcc cgaccccaac aagttcggcc tgcccgacaa caccgtgtac
     1861 gaccccaact cccagcggct ggtctgggcc tgcgtgggcg tggagatcgg cagaggccag
     1921 cccctgggcg tgggcctgag cggccacccc ctgtacaaca agctggacga caccgagaac
     1981 agccatgtgg ccagcgccgt ggacaccaag gacacccggg acaacgtgtc cgtggactac
     2041 aagcagaccc agctgtgcat catcggctgc gtgcctgcca ttggcgagca ctggaccaag
     2101 ggcaccgcct gcaagcccac caccgtggtg cagggcgact gcccccctct ggaactgatc
     2161 aacaccccca tcgaggacgg cgacatggtg gacaccggct acggcgccat ggacttcaag
     2221 ctgctgcagg acaacaagag cgaggtgccc ctggacatct gccagagcat ctgcaagtac
     2281 cccgactacc tgcagatgag cgccgacgcc tacggcgaca gcatgttctt ttgcctgcgg
     2341 cgggagcagg tgttcgcccg gcacttctgg aaccgcagcg gcaccatggg cgaccagctg
     2401 cccgagagcc tgtacatcaa gggcacagac atcagagcca accccggcag ctacctgtac
     2461 agccccagcc ccagcggctc tgtggtcacc tctgacagcc agctgttcaa caagccctac
     2521 tggctgcaca aggctcaggg cctgaacaac ggcatctgct ggcacaacca gctgtttctg
     2581 accgtggtgg ataccaccag aagcaccaac ctgagcgtct gcgccagcac cacctccagc
     2641 atccccaacg tgtacacccc taccagcttc aaagagtacg cccggcacgt cgaggaattc
     2701 gacctgcagt tcatcttcca gctgtgcaag atcaccctga ccaccgaagt gatgagctac
     2761 atccacaaca tgaacaccac catcctggaa gattggaact tcggcgtgac cccccctcct
     2821 accgccagcc tggtggacac atacagattc gtgcagtctg ccgccgtgac ctgccagaag
     2881 gataccgctc ctcccgtgaa gcaggacccc tacgacaagc tgaagttctg gcccgtggac
     2941 ctgaaagagc ggttcagcgc cgacctggac cagttccccc tgggccggaa gttcctgctg
     3001 cagctgggcg ccagacccaa gcctaccatc ggccccagaa agagagccgc ccctgcccca
     3061 accagcaccc cctcccccaa gagagtgaag cggagaaagt ccagccggaa gtgatagtct
     3121 agctggccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     3181 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     3241 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     3301 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggaaatgtta
     3361 attaactagc catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3421 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3481 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3541 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3601 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3661 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3721 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3781 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3841 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3901 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3961 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4021 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4081 cttttgctca catgttctta attaacctgc aggcgttaca taacttacgg taaatggccc
     4141 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     4201 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     4261 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     4321 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     4381 gcagtacatc tacgtattag tcatcgctat taccatgatg atgcggtttt ggcagtacat
     4441 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     4501 caatgggagt ttgttttgac tagtggagcc gagagtaatt catacaaaag gagggatcgc
     4561 cttcgcaagg ggagagccca gggaccgtcc ctaaattctc acagacccaa atccctgtag
     4621 ccgccccacg acagcgcgag gagcatgcgc ccagggctga gcgcgggtag atcagagcac
     4681 acaagctcac agtccccggc ggtgggggga ggggcgcgct gagcgggggc cagggagctg
     4741 gcgcggggca aactgggaaa gtggtgtcgt gtgctggctc cgccctcttc ccgagggtgg
     4801 gggagaacgg tatataagtg cggtagtcgc cttggacgtt ctttttcgca acgggtttgc
     4861 cgtcagaacg caggtgagtg gcgggtgtgg cttccgcggg ccccggagct ggagccctgc
     4921 tctgagcggg ccgggctgat atgcgagtgt cgtccgcagg gtttagctgt gagcattccc
     4981 acttcgagtg gcgggcggtg cgggggtgag agtgcgaggc ctagcggcaa ccccgtagcc
     5041 tcgcctcgtg tccggcttga ggcctagcgt ggtgtccgcc gccgcgtgcc actccggccg
     5101 cactatgcgt tttttgtcct tgctgccctc gattgccttc cagcagcatg ggctaacaaa
     5161 gggagggtgt ggggctcact cttaaggagc ccatgaagct tacgttggat aggaatggaa
     5221 gggcaggagg ggcgactggg gcccgcccgc cttcggagca catgtccgac gccacctgga
     5281 tggggcgagg cctgtggctt tccgaagcaa tcgggcgtga gtttagccta cctgggccat
     5341 gtggccctag cactgggcac ggtctggcct ggcggtgccg cgttcccttg cctcccaaca
     5401 agggtgaggc cgtcccgccc ggcaccagtt gcttgcgcgg aaagatggcc gctcccgggg
     5461 ccctgttgca aggagctcaa aatggaggac gcggcagccc ggtggagcgg gcgggtgagt
     5521 cacccacaca aaggaagagg gccttgcccc tcgccggccg ctgcttcctg tgaccccgtg
     5581 gtctatcggc cgcatagtca cctcgggctt ctcttgagca ccgctcgtcg cggcgggggg
     5641 aggggatcta atggcgttgg agtttgttca catttggtgg gtggagacta gtcaggccag
     5701 cctggcgctg gaagtcattc ttggaatttg cccctttgag tttggagcga ggctaattct
     5761 caagcctctt agcggttcaa aggtattttc taaacccgtt tccaggtgtt gtgaaagcca
     5821 ccgctaattc aaagcaatcc ggagtatacg gatccgccac catggtgtcc cacagagccg
     5881 ccagacggaa gcgggccagc gccaccgacc tgtacaagac ctgcaagcag gccggcacct
     5941 gccccagcga cgtgatcaac aaggtggagg gcaccacact ggccgacaag atcctgcagt
     6001 ggaccagcct gggcatcttc ctgggcggcc tgggcattgg caccggcagc ggcaccggcg
     6061 gcagaaccgg ctacatcccc ctcggcggca ggaccaacac catcgtggac gtgtccccag
     6121 ccaagccacc cgtggtcatc gagcccgtgg gccccaccga ccccagcatc gtgaccctgg
     6181 tggaggacag cagcgtgatc accagcggag cccctgcccc caccttcacc ggcaccagcg
     6241 gcttcgagat cagcaccagc agcaccacca cccctgccgt gctggacatc acacctacct
     6301 caagcgtgca gatcagcagc agctccttca tcaaccccgc cttcacagac ccctccgtca
     6361 tcgaggtgcc ccagaccggc gagatcagcg gcaacatcct gatcagcacc cctacctctg
     6421 gcgcccacgg ctacgaggaa atccccatgc agaccttcgc caccgagggc accggcctgg
     6481 aacccatcag ctccaccccc aaccccaccg tgcggagagt ggccggaccc cggctgtact
     6541 ctcgggccaa ccagcaggtc cgggtgtcca acgccgactt cctgacccgg cccagcacct
     6601 tcgtgaccta cgacaaccct gcctacgacc ccatcgacac caccctgacc ttcgacccct
     6661 ccagcgaggt gcccgacccc gacttcatgg acatcgtgcg gctgcacaga cccgccctga
     6721 ccagcagacg gtctaccgtg cggttcagcc ggctcggcca gcgcgccacc atgttcacca
     6781 gaagcggcaa gcagatcggc gccagagtgc acttctacca cgacatcagc cccatccccc
     6841 acgccgagga catcgagctg cagcccctgg tgtccagcca ggccgccacc gacgacatct
     6901 acgatatcta cgccgacatc accgacgagg cccctaccag caccgccaac accgccttta
     6961 ccatccccaa gagcagcttc cagagcctga gcctgaccag aagcgccagc tccaccttca
     7021 gcaacgtgac cgtgcccctg gccaccgcct gggacgtgcc tgtgaacacc ggccctgaca
     7081 tcgtgctgcc caacaccaac atcgtggagc ccacctacag caccaccccc ttcaccacca
     7141 tccagagcat caacatcgag ggcaccaact actttctgtg gcccatctac tattttctgc
     7201 cccggaagcg gaagcgggtg ccctacttct tcaccgacgg cagcatggcc ttttgatagt
     7261 ctaggagcag gtttccccaa tgacacaaaa cgtgcaactt gaaactccgc ctggtctttc
     7321 caggtctaga ggggtaacac tttgtactgc gtttggctcc acgctcgatc cactggcgag
     7381 tgttagtaac agcactgttg cttcgtagcg gagcatgacg gccgtgggaa ctcctccttg
     7441 gtaacaagga cccacggggc caaaagccac gcccacacgg gcccgtcatg tgtgcaaccc
     7501 cagcacggcg actttactgc gaaacccact ttaaagtgac attgaaactg gtacccacac
     7561 actggtgaca ggctaaggat gcccttcagg taccccgagg taacacgcga cactcgggat
     7621 ctgagaaggg gactggggct tctataaaag cgctcggttt aaaaagcttc tatgcctgaa
     7681 taggtgaccg gaggtcggca cctttccttt gcaattactg accctatgaa tacactgact
     7741 gtttgacaat taatcatcgg catagtatat cggcatagta taatacgact cactatagga
     7801 gggccaccat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     7861 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     7921 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     7981 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     8041 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     8101 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     8161 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     8221 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     8281 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     8341 tgcccgacgg cgaggatctc gtcgtgacac atggcgatgc ctgcttgccg aatatcatgg
     8401 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     8461 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     8521 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     8581 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgaa
     8641 ttcgctagga ttatccctaa tacctgccac cccactctta atcagtggtg gaagaacggt
     8701 ctcagaactg tttgtttcaa ttggccattt aagtttagta gtaaaagact ggttaatgat
     8761 aacaatgcat cgtaaaacct tcagaaggaa aggagaatgt tttgtggacc actttggttt
     8821 tcttttttgc gtgtggcagt tttaagttat tagtttttaa aatcagtact ttttaatgga
     8881 aacaacttga ccaaaaattt gtcacagaat tttgagaccc attaaaaaag ttaaatgaga
     8941 aacctgtgtg ttcctttggt caacaccgag acatttaggt gaaagacatc taattctggt
     9001 tttacgaatc tggaaacttc ttgaaaatgt aattcttgag ttaacacttc tgggtggaga
     9061 atagggttgt tttcccccca cataattgga aggggaagga atatcattta aagctatggg
     9121 agggttgctt tgattacaac actggagaga aatgcagcat gttgctgatt gcctgtcact
     9181 aaaacaggcc aaaaactgag tccttgggtt gcatagaaag ctg
//
