LOCUS       sequence-81720-e        9229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV68 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV68 L1L2
FEATURES             Location/Qualifiers
     source          1..9229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     regulatory      1582..1591
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3128..3249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3139..3158)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3193..3212
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3437..4025
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3926..3945
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4105..4408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4523..5828
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4865..5796
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     regulatory      5847..5856
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7272..7716
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7749..7795
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7787..7805
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7815..8609
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7815..8609
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7869..7888)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8479..8498
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8656..9228
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg gcactgtgga gagccagcga caacatggtg
     1621 tacctgcccc ctcccagcgt ggccaaggtg gtcaacaccg acgactacgt gacccggacc
     1681 ggcatgtact actacgccgg cacctctcgg ctcctgaccg tgggccaccc ctacttcaag
     1741 gtgcccatga gcggcggcag aaagcagggc atccccaagg tgtccgccta ccagtaccgg
     1801 gtgttcagag tgaccctgcc cgaccccaac aagttcagcg tgcccgagag caccctgtac
     1861 aaccccgaca cccagcggat ggtctgggcc tgcgtgggcg tggagatcgg cagaggccag
     1921 cccctgggcg tgggcctgag cggccacccc ctgtacaatc ggctggacga caccgagaac
     1981 agccccttca gcagcaacaa gaaccccaag gacagccggg acaacgtggc cgtggactgc
     2041 aagcagaccc agctgtgcat catcggctgc gtgcctgcca ttggcgagca ctgggccaag
     2101 ggcaagagct gcaagcccac caacgtgcag cagggcgact gcccccctct ggaactggtc
     2161 aacacaccca tcgaggacgg cgacatgatc gacaccggct acggcgccat ggacttcggc
     2221 accctgcagg aaaccaagag cgaggtcccc ctggacatct gccagagcgt gtgcaagtac
     2281 cccgactacc tgcagatgag cgccgacgtg tacggcgaca gcatgttctt ttgcctgcgg
     2341 cgggagcagc tgttcgcccg gcacttctgg aacagaggcg gcatggtcgg cgacaccatc
     2401 cccaccgaca tgtacatcaa gggcaccgac atcagagaga cacccagcag ctacgtgtac
     2461 gcccccagcc ccagcggcag catggtgtcc agcgacagcc agctgttcaa caagccctac
     2521 tggctgcaca aggcccaggg ccacaacaac ggcatctgct ggcacaacca gctgtttctg
     2581 accgtggtgg acaccaccag aagcaccaac ttcaccctga gcaccaccac cgacagcacc
     2641 gtgcccgccg tgtacgacag caataagttc aaagaatacg tgcggcacgt ggaggaatac
     2701 gacctgcagt tcatcttcca gctgtgtacc atcaccctgt ccaccgacgt gatgagctac
     2761 atccacacca tgaaccccgc catcctggac gactggaact tcggcgtggc ccctccccct
     2821 agcgccagcc tggtggatac ctacagatac ctgcagagcg ccgccatcac ctgccagaag
     2881 gacgcccctg cccccgtgaa gaaggacccc tacgacggcc tgaacttctg gaatgtggac
     2941 ctgaaagaga agttcagcag cgagctggac cagttccccc tgggccggaa gttcctgctg
     3001 caagccggcg tgcggagaag gcccaccatc ggccccagaa agcggaccgc caccgcagcc
     3061 acaacctcca cctccaagca caagcggaag cgggtgtcca agtgatagtc tagctggcca
     3121 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     3181 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     3241 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     3301 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggaaatgtt aattaactag
     3361 ccatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3421 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3481 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3541 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3601 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3661 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3721 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3781 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3841 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3901 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3961 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4021 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4081 acatgttctt aattaacctg caggcgttac ataacttacg gtaaatggcc cgcctggctg
     4141 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     4201 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     4261 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     4321 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     4381 ctacgtatta gtcatcgcta ttaccatgat gatgcggttt tggcagtaca tcaatgggcg
     4441 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     4501 tttgttttga ctagtggagc cgagagtaat tcatacaaaa ggagggatcg ccttcgcaag
     4561 gggagagccc agggaccgtc cctaaattct cacagaccca aatccctgta gccgccccac
     4621 gacagcgcga ggagcatgcg cccagggctg agcgcgggta gatcagagca cacaagctca
     4681 cagtccccgg cggtgggggg aggggcgcgc tgagcggggg ccagggagct ggcgcggggc
     4741 aaactgggaa agtggtgtcg tgtgctggct ccgccctctt cccgagggtg ggggagaacg
     4801 gtatataagt gcggtagtcg ccttggacgt tctttttcgc aacgggtttg ccgtcagaac
     4861 gcaggtgagt ggcgggtgtg gcttccgcgg gccccggagc tggagccctg ctctgagcgg
     4921 gccgggctga tatgcgagtg tcgtccgcag ggtttagctg tgagcattcc cacttcgagt
     4981 ggcgggcggt gcgggggtga gagtgcgagg cctagcggca accccgtagc ctcgcctcgt
     5041 gtccggcttg aggcctagcg tggtgtccgc cgccgcgtgc cactccggcc gcactatgcg
     5101 ttttttgtcc ttgctgccct cgattgcctt ccagcagcat gggctaacaa agggagggtg
     5161 tggggctcac tcttaaggag cccatgaagc ttacgttgga taggaatgga agggcaggag
     5221 gggcgactgg ggcccgcccg ccttcggagc acatgtccga cgccacctgg atggggcgag
     5281 gcctgtggct ttccgaagca atcgggcgtg agtttagcct acctgggcca tgtggcccta
     5341 gcactgggca cggtctggcc tggcggtgcc gcgttccctt gcctcccaac aagggtgagg
     5401 ccgtcccgcc cggcaccagt tgcttgcgcg gaaagatggc cgctcccggg gccctgttgc
     5461 aaggagctca aaatggagga cgcggcagcc cggtggagcg ggcgggtgag tcacccacac
     5521 aaaggaagag ggccttgccc ctcgccggcc gctgcttcct gtgaccccgt ggtctatcgg
     5581 ccgcatagtc acctcgggct tctcttgagc accgctcgtc gcggcggggg gaggggatct
     5641 aatggcgttg gagtttgttc acatttggtg ggtggagact agtcaggcca gcctggcgct
     5701 ggaagtcatt cttggaattt gcccctttga gtttggagcg aggctaattc tcaagcctct
     5761 tagcggttca aaggtatttt ctaaacccgt ttccaggtgt tgtgaaagcc accgctaatt
     5821 caaagcaatc cggagtatac ggatccgcca ccatggtgtc ccacagagcc gccagacgga
     5881 agcgggccag cgccaccgac ctgtacaaga cctgcaagca gagcggcacc tgccccagcg
     5941 acgtgatcaa caaggtggag ggcaccacac tggccgacaa gatcctgcag tggaccagcc
     6001 tgggcatctt cctgggcggc ctgggcattg gcaccggcag cggcacaggc ggcagagccg
     6061 gctacatccc cctcggcggc aagcccaaca ccgtggtgga cgtgtccccc gccagacccc
     6121 ccgtggtcat cgagcccgtg ggccccaccg agcccagcat cgtgcagctg gtcgaggaca
     6181 gcagcgtgat cacctctggc acacccgtcc ccaccttcac cggcaccagc ggcttcgaga
     6241 tcaccagcag ctccaccacc acccctgccg tgctggacat cacccccagc agcggcagcg
     6301 tgcaggtgtc cagcaccagc ttcaccaacc ccgccttcac cgaccccacc atcatcgagg
     6361 tgccccagac cggcgaggtg tccggcaacg tgttcgtgag cacccccacc tccggcactc
     6421 acggctatga ggaaatcccc atgcaggtgt tcgccaccca cggcacaggc acagaaccta
     6481 tcagcagcac ccccatccct ggcgtgtctc gggtggcagg accccggctc tactctaggg
     6541 ctcaccagca ggtccgggtg tccaacttcg acttcgtgac ccacccctct agcttcgtca
     6601 ccttcgacaa ccctgccttc gagcctgtgg acaccactct gacctatgag cccgccgata
     6661 tcgcccccga ccccgacttc ctggacatcg tgcggctgca cagacccgcc ctgaccagca
     6721 gacggggcac cgtgcggttc agcagagtgg gcaagaaagc caccatgttc accaggcggg
     6781 ggacccagat cggcgcccag gtgcactact accacgacat cagcaatatc acaccagccg
     6841 acagcatcga gctgcagccc ctggtggccc ccgagcaggc cgaccccatg gacaacctgt
     6901 acgacatcta cgctcccgat actgacaaca ccaccgtgct ggataccgcc ttccacaacg
     6961 ccacctttac caccagatcc cacatcagcg tgcccagcct ggccagcgcc gccagcacca
     7021 cctacacaaa caccaccatc cctctgggca ccgcctggaa cacccccgtg aacaccggcc
     7081 ctgacgtggt cctgcccagc acaacacccc agctgcctct gaccccctcc acccccatcg
     7141 acaccacctt cgccatcacc atctacggca gcaattacta cctcctgccc ctgctgttct
     7201 tcctgctgaa gaagcggaag cacctgccct actttttcac cgacggcatc gtggccagct
     7261 gatagtctag gagcaggttt ccccaatgac acaaaacgtg caacttgaaa ctccgcctgg
     7321 tctttccagg tctagagggg taacactttg tactgcgttt ggctccacgc tcgatccact
     7381 ggcgagtgtt agtaacagca ctgttgcttc gtagcggagc atgacggccg tgggaactcc
     7441 tccttggtaa caaggaccca cggggccaaa agccacgccc acacgggccc gtcatgtgtg
     7501 caaccccagc acggcgactt tactgcgaaa cccactttaa agtgacattg aaactggtac
     7561 ccacacactg gtgacaggct aaggatgccc ttcaggtacc ccgaggtaac acgcgacact
     7621 cgggatctga gaaggggact ggggcttcta taaaagcgct cggtttaaaa agcttctatg
     7681 cctgaatagg tgaccggagg tcggcacctt tcctttgcaa ttactgaccc tatgaataca
     7741 ctgactgttt gacaattaat catcggcata gtatatcggc atagtataat acgactcact
     7801 ataggagggc caccatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     7861 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     7921 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     7981 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     8041 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     8101 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     8161 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     8221 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     8281 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     8341 cgagcatgcc cgacggcgag gatctcgtcg tgacacatgg cgatgcctgc ttgccgaata
     8401 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     8461 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     8521 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     8581 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     8641 agcgaattcg ctaggattat ccctaatacc tgccacccca ctcttaatca gtggtggaag
     8701 aacggtctca gaactgtttg tttcaattgg ccatttaagt ttagtagtaa aagactggtt
     8761 aatgataaca atgcatcgta aaaccttcag aaggaaagga gaatgttttg tggaccactt
     8821 tggttttctt ttttgcgtgt ggcagtttta agttattagt ttttaaaatc agtacttttt
     8881 aatggaaaca acttgaccaa aaatttgtca cagaattttg agacccatta aaaaagttaa
     8941 atgagaaacc tgtgtgttcc tttggtcaac accgagacat ttaggtgaaa gacatctaat
     9001 tctggtttta cgaatctgga aacttcttga aaatgtaatt cttgagttaa cacttctggg
     9061 tggagaatag ggttgttttc cccccacata attggaaggg gaaggaatat catttaaagc
     9121 tatgggaggg ttgctttgat tacaacactg gagagaaatg cagcatgttg ctgattgcct
     9181 gtcactaaaa caggccaaaa actgagtcct tgggttgcat agaaagctg
//
