LOCUS       sequence-81721-e        9241 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV73 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV73 L1L2
FEATURES             Location/Qualifiers
     source          1..9241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     polyA_signal    3122..3243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3133..3152)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3187..3206
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3431..4019
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3920..3939
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4099..4402
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4517..5822
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4859..5790
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     misc_feature    7284..7728
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7761..7807
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7799..7817
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7827..8621
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7827..8621
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7881..7900)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8491..8510
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8668..9240
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg tggagaccca ccgacgccaa ggtgtacctg
     1621 ccccccgtgt ccgtgagcaa ggtggtgtcc accgacgagt acgtgacccg gaccaacatc
     1681 tactactacg ccggcagcac acggctgctc gccgtgggcc acccctactt ccccatcaag
     1741 gacagccaga agagaaagac cattgtccct aaggtgtccg gcctccagta tcgggtgttc
     1801 cggctgagac tgcccgaccc caacaagttc ggcttccccg acgccagctt ctacaacccc
     1861 gacaaagaac ggctggtctg ggcctgctct ggcgtcgaag tgggcagagg ccagcccctg
     1921 ggcatcggca ccagcggcaa ccccttcatg aacaagctgg acgacaccga gaacgccccc
     1981 aagtatatcg ccggccagaa caccgacggc cgggagtgca tgagcgtgga ctacaagcag
     2041 acccagctgt gcatcctggg ctgtagaccc cctctgggcg agcactgggg ccctggcacc
     2101 ccctgcacca gccagaccgt gaacaccggc gactgccctc ctctggaact gaagaacacc
     2161 cccatccagg acggcgacat gatcgacgtg ggcttcggcg ccatggactt caaggccctg
     2221 caggccaaca agagcgacgt gcccatcgac atcagcaaca ccacctgcaa gtaccccgac
     2281 tacctgggca tggccgccga cccctacggc gacagcatgt ggttctacct gcggcgggag
     2341 cagatgttcg tgcggcacct gttcaacaga gccggcgaca ccggcgataa gatccccgac
     2401 gacctgatga tcaagggcac cggcaacacc gccaccccca gcagctgcgt gttctacccc
     2461 accccctccg gctctatggt gtcctccgat gctcagctgt tcaataagcc ctactggctg
     2521 cagaaggccc agggccagaa caacggcatc tgctggcaca accagctgtt tctgaccgtg
     2581 gtcgacacaa ccagaagcac caacttcagc gtctgcgtgg gcactcaggc ctctagctcc
     2641 accaccacct acgccaacag caacttcaaa gagtacctgc ggcacgccga ggaattcgac
     2701 ctgcagttcg tgttccagct gtgcaagatc agcctgacca ccgaagtgat gacctacatc
     2761 cacagcatga acagcaccat cctggaagag tggaacttcg gcctgacccc ccctcctagc
     2821 ggcaccctgg aagagacata cagatacgtg accagccagg ccatcagctg ccagaggccc
     2881 cagcccccca aagagacaga ggacccctac gccaagctgt ccttctggga cgtggacctg
     2941 aaagagaagt tcagcgccga gctggaccag ttccccctgg gccggaagtt cctgctgcag
     3001 ctgggcatga gagccagacc caagctgcag gcctccaagc ggagcgccag cgccaccaca
     3061 agcgccaccc ctaagaagaa gcgggccaag cggatctgat agtctagctg gccagacatg
     3121 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     3181 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     3241 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     3301 ttttaaagca agtaaaacct ctacaaatgt ggtatggaaa tgttaattaa ctagccatga
     3361 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3421 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3481 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3541 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3601 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3661 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3721 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3781 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3841 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3901 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3961 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4021 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4081 tcttaattaa cctgcaggcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     4141 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     4201 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     4261 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     4321 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     4381 attagtcatc gctattacca tgatgatgcg gttttggcag tacatcaatg ggcgtggata
     4441 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     4501 ttgactagtg gagccgagag taattcatac aaaaggaggg atcgccttcg caaggggaga
     4561 gcccagggac cgtccctaaa ttctcacaga cccaaatccc tgtagccgcc ccacgacagc
     4621 gcgaggagca tgcgcccagg gctgagcgcg ggtagatcag agcacacaag ctcacagtcc
     4681 ccggcggtgg ggggaggggc gcgctgagcg ggggccaggg agctggcgcg gggcaaactg
     4741 ggaaagtggt gtcgtgtgct ggctccgccc tcttcccgag ggtgggggag aacggtatat
     4801 aagtgcggta gtcgccttgg acgttctttt tcgcaacggg tttgccgtca gaacgcaggt
     4861 gagtggcggg tgtggcttcc gcgggccccg gagctggagc cctgctctga gcgggccggg
     4921 ctgatatgcg agtgtcgtcc gcagggttta gctgtgagca ttcccacttc gagtggcggg
     4981 cggtgcgggg gtgagagtgc gaggcctagc ggcaaccccg tagcctcgcc tcgtgtccgg
     5041 cttgaggcct agcgtggtgt ccgccgccgc gtgccactcc ggccgcacta tgcgtttttt
     5101 gtccttgctg ccctcgattg ccttccagca gcatgggcta acaaagggag ggtgtggggc
     5161 tcactcttaa ggagcccatg aagcttacgt tggataggaa tggaagggca ggaggggcga
     5221 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgt
     5281 ggctttccga agcaatcggg cgtgagttta gcctacctgg gccatgtggc cctagcactg
     5341 ggcacggtct ggcctggcgg tgccgcgttc ccttgcctcc caacaagggt gaggccgtcc
     5401 cgcccggcac cagttgcttg cgcggaaaga tggccgctcc cggggccctg ttgcaaggag
     5461 ctcaaaatgg aggacgcggc agcccggtgg agcgggcggg tgagtcaccc acacaaagga
     5521 agagggcctt gcccctcgcc ggccgctgct tcctgtgacc ccgtggtcta tcggccgcat
     5581 agtcacctcg ggcttctctt gagcaccgct cgtcgcggcg gggggagggg atctaatggc
     5641 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     5701 cattcttgga atttgcccct ttgagtttgg agcgaggcta attctcaagc ctcttagcgg
     5761 ttcaaaggta ttttctaaac ccgtttccag gtgttgtgaa agccaccgct aattcaaagc
     5821 aatccggagt atacggatcc gccaccatgc ggcggaagcg ggacacccac atccggaaga
     5881 agcgggccag cgccacccag ctgtacaaga cctgcaagca ggccggcacc tgcccccctg
     5941 acgtgatccc caaggtggag ggcagcacaa tcgccgacaa catcctgaag tacggcagca
     6001 tcggcgtgtt cttcggcggc ctgggcatcg gcagcggcag cggatctggc ggcagaaccg
     6061 gctacgtgcc cctgagcacc ggcaccccca gcaagcccgt ggagatcccc ctgcagccca
     6121 tccggcccag cgtcgtcacc agcgtcggcc ccagcgacag cagcatcgtg tccctggtgg
     6181 aggaaagcag cttcatcgag agcggcatcc ctggccccac ctccatcgtg cccagcacca
     6241 gcggcttcga catcaccacc tccgtgaaca gcacccccgc catcatcgac gtgtccgcca
     6301 tcagcgacac cacccagatc agcgtgacca ccttcaagaa ccccactttc accgatccca
     6361 gcgtgctgca gccccctccc cccctggaag ccagcggcag actgctgttc agcaacgaca
     6421 ccgtgaccac ccacagctac gagaacatcc ccctggacac ctttgtggtc accaccgacc
     6481 acaactccat cgtgtcctcc acccccatcc ccggcagaca gcctgccgcc agactgggcc
     6541 tgtacggcag agccatccag caggtcaagg tggtggaccc cgccttcctg accaccccca
     6601 cccggctggt cacctacgac aaccctgcct tcgagggcct gcaggacacc accctcgaat
     6661 tccagcacag cgacctgcac aacgcccccg acagcgactt cctggacatc gtgaagctgc
     6721 acagacccgc cctgaccagc agaaagaccg gcatccgggt gtcccggctg ggccagagag
     6781 ccaccctgtc caccagaagc ggcaagagaa tcggcgccaa ggtgcacttc taccacgaca
     6841 tcagccccat ccccaccaac gacatcgaga tgcagcccct ggtcaccccc cagaccccca
     6901 gcatcgtgac cggcagcagc atcaacgacg gcctgtacga cgtgttcctg gacaacgacg
     6961 tggaggaaac agtgctgcag cagacctaca cccctaccag catccacagc aacagcctgg
     7021 tgtccagcga catctccacc gccaccgcca acaccaccat ccctttcagc acaggcctcg
     7081 acacacaccc aggcccagat atcgccctgc ccctgccctc taccgagaca atcttcaccc
     7141 ctatcgtgcc tctgcagcct gccggcccta tctacatcta cggcagcggc ttcatcctgc
     7201 accccagcta ctacctgctg aagcggaagc ggaagagact gagctacagc ttcaccgacg
     7261 tggccaccta ctgatagtct aggagcaggt ttccccaatg acacaaaacg tgcaacttga
     7321 aactccgcct ggtctttcca ggtctagagg ggtaacactt tgtactgcgt ttggctccac
     7381 gctcgatcca ctggcgagtg ttagtaacag cactgttgct tcgtagcgga gcatgacggc
     7441 cgtgggaact cctccttggt aacaaggacc cacggggcca aaagccacgc ccacacgggc
     7501 ccgtcatgtg tgcaacccca gcacggcgac tttactgcga aacccacttt aaagtgacat
     7561 tgaaactggt acccacacac tggtgacagg ctaaggatgc ccttcaggta ccccgaggta
     7621 acacgcgaca ctcgggatct gagaagggga ctggggcttc tataaaagcg ctcggtttaa
     7681 aaagcttcta tgcctgaata ggtgaccgga ggtcggcacc tttcctttgc aattactgac
     7741 cctatgaata cactgactgt ttgacaatta atcatcggca tagtatatcg gcatagtata
     7801 atacgactca ctataggagg gccaccatga ttgaacaaga tggattgcac gcaggttctc
     7861 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     7921 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     7981 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     8041 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     8101 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     8161 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     8221 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     8281 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     8341 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacacat ggcgatgcct
     8401 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     8461 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     8521 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     8581 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     8641 aatgaccgac caagcgaatt cgctaggatt atccctaata cctgccaccc cactcttaat
     8701 cagtggtgga agaacggtct cagaactgtt tgtttcaatt ggccatttaa gtttagtagt
     8761 aaaagactgg ttaatgataa caatgcatcg taaaaccttc agaaggaaag gagaatgttt
     8821 tgtggaccac tttggttttc ttttttgcgt gtggcagttt taagttatta gtttttaaaa
     8881 tcagtacttt ttaatggaaa caacttgacc aaaaatttgt cacagaattt tgagacccat
     8941 taaaaaagtt aaatgagaaa cctgtgtgtt cctttggtca acaccgagac atttaggtga
     9001 aagacatcta attctggttt tacgaatctg gaaacttctt gaaaatgtaa ttcttgagtt
     9061 aacacttctg ggtggagaat agggttgttt tccccccaca taattggaag gggaaggaat
     9121 atcatttaaa gctatgggag ggttgctttg attacaacac tggagagaaa tgcagcatgt
     9181 tgctgattgc ctgtcactaa aacaggccaa aaactgagtc cttgggttgc atagaaagct
     9241 g
//
