LOCUS       sequence-81722-e        9184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV6 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV6 L1L2
FEATURES             Location/Qualifiers
     source          1..9184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     polyA_signal    3113..3234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3124..3143)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3178..3197
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3422..4010
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3911..3930
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4090..4393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4508..5813
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4850..5781
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     regulatory      5832..5841
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7227..7671
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7704..7750
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7742..7760
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7770..8564
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7770..8564
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7824..7843)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8434..8453
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8611..9183
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg tggaggccca gcgacagcac cgtgtacgtg
     1621 ccccccccca accccgtgag caaggtggtg gccaccgacg cctacgtgac caggaccaac
     1681 atcttctacc acgccagcag cagcaggctg ctggccgtgg gccaccccta cttcagcatc
     1741 aagagggcca acaagaccgt ggtgcccaag gtgagcggct accagtacag ggtgttcaag
     1801 gtggtgctgc ccgaccccaa caagttcgcc ctgcccgaca gcagcctgtt cgaccccacc
     1861 acccagaggc tggtgtgggc ctgcaccggc ctggaggtgg gcaggggcca gcccctgggc
     1921 gtgggcgtga gcggccaccc cttcctgaac aagtacgacg acgtggagaa cagcggcagc
     1981 ggcggcaacc ccggccagga caacagggtg aacgtgggca tggactacaa gcagacccag
     2041 ctgtgcatgg tgggctgcgc cccccccctg ggcgagcact ggggcaaggg caagcagtgc
     2101 accaacaccc ccgtgcaggc cggcgactgc ccccccctgg agctgatcac cagcgtgatc
     2161 caggacggcg acatggtgga caccggcttc ggcgccatga acttcgccga cctgcagacc
     2221 aacaagagcg acgtgcccat cgacatctgc ggcaccacct gcaagtaccc cgactacctg
     2281 cagatggccg ccgaccccta cggcgacagg ctgttcttct tcctgaggaa ggagcagatg
     2341 ttcgccaggc acttcttcaa cagggccggc gaggtgggcg agcccgtgcc cgacaccctg
     2401 atcatcaagg gcagcggcaa caggaccagc gtgggcagca gcatctacgt gaacaccccc
     2461 agcggcagcc tggtgagcag cgaggcccag ctgttcaaca agccctactg gctgcagaag
     2521 gcccagggcc acaacaacgg catctgctgg ggcaaccagc tgttcgtgac cgtggtggac
     2581 accaccagga gcaccaacat gaccctgtgc gccagcgtga ccaccagcag cacctacacc
     2641 aacagcgact acaaggagta catgaggcac gtggaggagt acgacctgca gttcatcttc
     2701 cagctgtgca gcatcaccct gagcgccgag gtgatggcct acatccacac catgaacccc
     2761 agcgtgctgg aggactggaa cttcggcctg agcccccccc ccaacggcac cctggaggac
     2821 acctacaggt acgtgcagag ccaggccatc acctgccaga agcccacccc cgagaaggag
     2881 aagcccgacc cctacaagaa cctgagcttc tgggaggtga acctgaagga gaagttcagc
     2941 agcgagctgg accagtaccc cctgggcagg aagttcctgc tgcagagcgg ctacaggggc
     3001 aggagcagca tcaggaccgg cgtgaagagg cccgccgtga gcaaggccag cgccgccccc
     3061 aagaggaaga gggccaagac caagaggtaa tagtctagct ggccagacat gataagatac
     3121 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     3181 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     3241 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     3301 aagtaaaacc tctacaaatg tggtatggaa atgttaatta actagccatg accaaaatcc
     3361 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3421 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3481 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3541 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3601 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3661 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3721 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3781 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3841 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3901 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3961 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4021 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttcttaatta
     4081 acctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     4141 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     4201 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     4261 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     4321 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     4381 cgctattacc atgatgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     4441 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttgactagt
     4501 ggagccgaga gtaattcata caaaaggagg gatcgccttc gcaaggggag agcccaggga
     4561 ccgtccctaa attctcacag acccaaatcc ctgtagccgc cccacgacag cgcgaggagc
     4621 atgcgcccag ggctgagcgc gggtagatca gagcacacaa gctcacagtc cccggcggtg
     4681 gggggagggg cgcgctgagc gggggccagg gagctggcgc ggggcaaact gggaaagtgg
     4741 tgtcgtgtgc tggctccgcc ctcttcccga gggtggggga gaacggtata taagtgcggt
     4801 agtcgccttg gacgttcttt ttcgcaacgg gtttgccgtc agaacgcagg tgagtggcgg
     4861 gtgtggcttc cgcgggcccc ggagctggag ccctgctctg agcgggccgg gctgatatgc
     4921 gagtgtcgtc cgcagggttt agctgtgagc attcccactt cgagtggcgg gcggtgcggg
     4981 ggtgagagtg cgaggcctag cggcaacccc gtagcctcgc ctcgtgtccg gcttgaggcc
     5041 tagcgtggtg tccgccgccg cgtgccactc cggccgcact atgcgttttt tgtccttgct
     5101 gccctcgatt gccttccagc agcatgggct aacaaaggga gggtgtgggg ctcactctta
     5161 aggagcccat gaagcttacg ttggatagga atggaagggc aggaggggcg actggggccc
     5221 gcccgccttc ggagcacatg tccgacgcca cctggatggg gcgaggcctg tggctttccg
     5281 aagcaatcgg gcgtgagttt agcctacctg ggccatgtgg ccctagcact gggcacggtc
     5341 tggcctggcg gtgccgcgtt cccttgcctc ccaacaaggg tgaggccgtc ccgcccggca
     5401 ccagttgctt gcgcggaaag atggccgctc ccggggccct gttgcaagga gctcaaaatg
     5461 gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg aagagggcct
     5521 tgcccctcgc cggccgctgc ttcctgtgac cccgtggtct atcggccgca tagtcacctc
     5581 gggcttctct tgagcaccgc tcgtcgcggc ggggggaggg gatctaatgg cgttggagtt
     5641 tgttcacatt tggtgggtgg agactagtca ggccagcctg gcgctggaag tcattcttgg
     5701 aatttgcccc tttgagtttg gagcgaggct aattctcaag cctcttagcg gttcaaaggt
     5761 attttctaaa cccgtttcca ggtgttgtga aagccaccgc taattcaaag caatccggag
     5821 tatacggatc cgccaccatg gcccacagca gggccaggag gaggaagagg gccagcgcca
     5881 cccagctgta ccagacctgc aagctgaccg gcacctgccc ccccgacgtg atccccaagg
     5941 tggagcacaa caccatcgcc gaccagatcc tgaagtgggg cagcctgggc gtgttcttcg
     6001 gcggcctggg catcggcacc ggcagcggca ccggcggcag gaccggctac gtgcccctgc
     6061 agaccagcgc caagcccagc atcaccagcg gccccatggc caggcccccc gtggtggtgg
     6121 agcccgtggc ccccagcgac cccagcatcg tgagcctgat cgaggagagc gccatcatca
     6181 acgccggcgc ccccgagatc gtgccccccg cccacggcgg cttcaccatc accagcagcg
     6241 agaccaccac ccccgccatc ctggacgtga gcgtcaccag ccacaccacc accagcatct
     6301 tcaggaaccc cgtgttcacc gagcccagcg tgacccagcc ccagcccccc gtggaggcca
     6361 acggccacat cctgatcagc gcccccaccg tgaccagcca ccccatcgag gagatccccc
     6421 tggacacctt cgtggtgagc agcagcgaca gcggccccac cagcagcacc cccgtgcccg
     6481 gcaccgcccc caggcccagg gtgggcctgt acagcagggc cctgcaccag gtgcaggtga
     6541 ccgaccccgc cttcctgagc accccccaga ggctgatcac ctacgacaac cccgtgtacg
     6601 agggcgagga cgtgagcgtg cagttcagcc acgacagcat ccacaacgcc cccgacgagg
     6661 ccttcatgga catcatcagg ctgcacaggc ccgccatcgc cagcaggagg ggcctggtga
     6721 ggtacagcag gatcggccag aggggcagca tgcacaccag gagcggcaag cacatcggcg
     6781 ccaggattca ctacttctac gacatcagcc ccatcgccca agccgccgag gagatcgaga
     6841 tgcaccccct ggtggccgcc caggacgaca ccttcgacat ctacgccgag agcttcgagc
     6901 ccggcatcaa ccccacccag caccccgtga ccaacatcag cgacacctac ctgaccagca
     6961 cccccaacac cgtgacccag ccctggggca acaccaccgt gcccctgagc ctgcccaacg
     7021 accccttcct gcagagcggc cccgacatca ccttccccac cgcccccatg ggcaccccct
     7081 tcagccccgt gacccccgcc ctgcccaccg gccccgtgtt catcaccggc agcggcttct
     7141 acctgcaccc cgcctggtac ttcgccagga agaggaggaa gaggattccc ctgttcttca
     7201 gcgacgtggc cgcctaatag tctaggagca ggtttcccca atgacacaaa acgtgcaact
     7261 tgaaactccg cctggtcttt ccaggtctag aggggtaaca ctttgtactg cgtttggctc
     7321 cacgctcgat ccactggcga gtgttagtaa cagcactgtt gcttcgtagc ggagcatgac
     7381 ggccgtggga actcctcctt ggtaacaagg acccacgggg ccaaaagcca cgcccacacg
     7441 ggcccgtcat gtgtgcaacc ccagcacggc gactttactg cgaaacccac tttaaagtga
     7501 cattgaaact ggtacccaca cactggtgac aggctaagga tgcccttcag gtaccccgag
     7561 gtaacacgcg acactcggga tctgagaagg ggactggggc ttctataaaa gcgctcggtt
     7621 taaaaagctt ctatgcctga ataggtgacc ggaggtcggc acctttcctt tgcaattact
     7681 gaccctatga atacactgac tgtttgacaa ttaatcatcg gcatagtata tcggcatagt
     7741 ataatacgac tcactatagg agggccacca tgattgaaca agatggattg cacgcaggtt
     7801 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     7861 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     7921 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     7981 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     8041 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     8101 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     8161 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     8221 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     8281 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgaca catggcgatg
     8341 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     8401 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     8461 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     8521 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     8581 cgaaatgacc gaccaagcga attcgctagg attatcccta atacctgcca ccccactctt
     8641 aatcagtggt ggaagaacgg tctcagaact gtttgtttca attggccatt taagtttagt
     8701 agtaaaagac tggttaatga taacaatgca tcgtaaaacc ttcagaagga aaggagaatg
     8761 ttttgtggac cactttggtt ttcttttttg cgtgtggcag ttttaagtta ttagttttta
     8821 aaatcagtac tttttaatgg aaacaacttg accaaaaatt tgtcacagaa ttttgagacc
     8881 cattaaaaaa gttaaatgag aaacctgtgt gttcctttgg tcaacaccga gacatttagg
     8941 tgaaagacat ctaattctgg ttttacgaat ctggaaactt cttgaaaatg taattcttga
     9001 gttaacactt ctgggtggag aatagggttg ttttcccccc acataattgg aaggggaagg
     9061 aatatcattt aaagctatgg gagggttgct ttgattacaa cactggagag aaatgcagca
     9121 tgttgctgat tgcctgtcac taaaacaggc caaaaactga gtccttgggt tgcatagaaa
     9181 gctg
//
