LOCUS       sequence-81723-e        9175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV11 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9175)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV11 L1L2
FEATURES             Location/Qualifiers
     source          1..9175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     polyA_signal    3116..3237
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3127..3146)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3181..3200
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3425..4013
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3914..3933
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4093..4396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4511..5816
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4853..5784
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     misc_feature    7218..7662
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7695..7741
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7733..7751
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7761..8555
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7761..8555
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7815..7834)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8425..8444
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8602..9174
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg tggcgaccct cagatagcac tgtatacgtc
     1621 cccccgccaa accccgtcag caaggttgta gcgaccgacg cctacgtcaa gaggaccaat
     1681 attttctatc acgccagctc aagtaggttg ctggcagtcg gccatccata ctattcaatt
     1741 aagaaagtta acaagactgt ggtgccgaaa gtgagcggct accagtacag agtatttaaa
     1801 gtcgtattgc cggaccccaa caagttcgcc ctccctgatt cttctctttt tgatcccact
     1861 acgcagcggt tggtatgggc ttgcaccggg ctcgaagtgg gacgggggca gcctctcggc
     1921 gtgggcgtga gcgggcatcc cctgctgaac aagtacgatg acgtggagaa ttctggtggg
     1981 tatggcggta acccaggcca ggataatcgc gtgaatgtgg gcatggacta caagcagact
     2041 cagctgtgta tggtgggctg cgctccacct ctgggggaac actggggcaa gggaacccag
     2101 tgcagcaata catccgtgca gaacggcgac tgtcctccac ttgaactcat cacgagcgtc
     2161 atccaggacg gagatatggt ggatacaggc tttggtgcaa tgaatttcgc cgacctccag
     2221 acaaacaaga gtgacgtgcc tcttgacatt tgtggaactg tttgcaaata ccctgactat
     2281 cttcagatgg ctgctgaccc ctatggagat aggcttttct tttatctgcg gaaagaacag
     2341 atgttcgcca gacatttctt taaccgggca gggaccgttg gggagcccgt tcccgacgat
     2401 ctcttggtga aagggggaaa caaccggagt tccgtcgcct ccagtatcta tgtgcataca
     2461 ccttccggta gcctggtttc ctccgaggcc cagctgttca acaaaccata ttggctccag
     2521 aaagcgcaag gtcacaataa cgggatatgt tggggaaatc acttgtttgt gaccgttgtg
     2581 gatactaccc gctctaccaa tatgaccctg tgcgcttctg tcagcaagtc cgcaacatat
     2641 acaaattccg actacaaaga gtatatgaga cacgtggagg agtttgactt gcaattcatt
     2701 ttccaactgt gctccatcac tctgagtgct gaggtcatgg cctacataca caccatgaac
     2761 ccttctgtgc tggaagactg gaatttcggg ctcagtccac ctccaaacgg cacactcgag
     2821 gatacgtata gatacgttca aagccaggcc atcacatgtc agaaacctac ccccgaaaag
     2881 gagaagcagg acccatacaa ggatatgtca ttttgggaag tcaatctgaa ggaaaagttc
     2941 agctctgagc tggatcaatt cccactgggc cgcaagtttc ttctgcaaag tggatacagg
     3001 ggaagaactt cagcacgcac cggaatcaaa cgacccgccg tgtcaaagcc gtcaacagca
     3061 cccaaaagga agcggacgaa aacaaagaaa taatagtcta gctggccaga catgataaga
     3121 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     3181 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     3241 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     3301 agcaagtaaa acctctacaa atgtggtatg gaaatgttaa ttaactagcc atgaccaaaa
     3361 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3421 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3481 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3541 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3601 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3661 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3721 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3781 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3841 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3901 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3961 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4021 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttcttaa
     4081 ttaacctgca ggcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     4141 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     4201 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     4261 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     4321 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     4381 catcgctatt accatgatga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     4441 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttgact
     4501 agtggagccg agagtaattc atacaaaagg agggatcgcc ttcgcaaggg gagagcccag
     4561 ggaccgtccc taaattctca cagacccaaa tccctgtagc cgccccacga cagcgcgagg
     4621 agcatgcgcc cagggctgag cgcgggtaga tcagagcaca caagctcaca gtccccggcg
     4681 gtggggggag gggcgcgctg agcgggggcc agggagctgg cgcggggcaa actgggaaag
     4741 tggtgtcgtg tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc
     4801 ggtagtcgcc ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagtgg
     4861 cgggtgtggc ttccgcgggc cccggagctg gagccctgct ctgagcgggc cgggctgata
     4921 tgcgagtgtc gtccgcaggg tttagctgtg agcattccca cttcgagtgg cgggcggtgc
     4981 gggggtgaga gtgcgaggcc tagcggcaac cccgtagcct cgcctcgtgt ccggcttgag
     5041 gcctagcgtg gtgtccgccg ccgcgtgcca ctccggccgc actatgcgtt ttttgtcctt
     5101 gctgccctcg attgccttcc agcagcatgg gctaacaaag ggagggtgtg gggctcactc
     5161 ttaaggagcc catgaagctt acgttggata ggaatggaag ggcaggaggg gcgactgggg
     5221 cccgcccgcc ttcggagcac atgtccgacg ccacctggat ggggcgaggc ctgtggcttt
     5281 ccgaagcaat cgggcgtgag tttagcctac ctgggccatg tggccctagc actgggcacg
     5341 gtctggcctg gcggtgccgc gttcccttgc ctcccaacaa gggtgaggcc gtcccgcccg
     5401 gcaccagttg cttgcgcgga aagatggccg ctcccggggc cctgttgcaa ggagctcaaa
     5461 atggaggacg cggcagcccg gtggagcggg cgggtgagtc acccacacaa aggaagaggg
     5521 ccttgcccct cgccggccgc tgcttcctgt gaccccgtgg tctatcggcc gcatagtcac
     5581 ctcgggcttc tcttgagcac cgctcgtcgc ggcgggggga ggggatctaa tggcgttgga
     5641 gtttgttcac atttggtggg tggagactag tcaggccagc ctggcgctgg aagtcattct
     5701 tggaatttgc ccctttgagt ttggagcgag gctaattctc aagcctctta gcggttcaaa
     5761 ggtattttct aaacccgttt ccaggtgttg tgaaagccac cgctaattca aagcaatccg
     5821 gagtatacgg atccgccacc atgaaaccca gagccagaag aagaaagagg gcatcagcaa
     5881 cacagctgta ccagacctgt aaggcgacag gcacctgccc gccagacgtg attcctaaag
     5941 tagagcacac cactatagcc gaccagattt tgaagtgggg gtccctcggc gtgtttttcg
     6001 gcggtctcgg gatcggaacc ggagctgggt ctggtggcag agcaggatac attcctctgg
     6061 gctcttcccc caagcccgca attacagggg gtccagccgc taggcctccc gtcctggtgg
     6121 agcctgtggc cccgtccgac cccagtacag tgagtttgat agaagagtcc gcaatcatca
     6181 acgccggcgc ccctgaagtt gtcccaccca cacagggagg cttcaccatc acttcctcag
     6241 aaagtactac tccggctatt ctggacgtgt ctgtgaccaa ccatacaacc acctctgtct
     6301 tccagaatcc cctctttact gagccgagtg ttattcagcc ccagccccca gtcgaagcct
     6361 caggacacat cttgatctca gctccaacga tcaccagcca gcatgtcgag gacatccccc
     6421 ttgacacatt tgtcgtgtca agcagtgata gtggcccgac ttcctccact cctctgcccc
     6481 gcgccttccc tcgcccccgg gtgggtcttt attctcgggc ccttcaacaa gtacaggtta
     6541 ctgatccagc gttcctgagc acaccacagc gcctcgtgac gtacgataat cctgtgtacg
     6601 agggggagga tgtttccctg caatttaccc acgagagcat ccataatgct cctgatgagg
     6661 ctttcatgga tattatacgc ttgcaccggc cagccatcac atcaaggaga ggtttggtgc
     6721 gattcagccg aatcggccag aggggcagca tgtacacccg gtctgggcag cacatcgggg
     6781 ctcggattca ctattttcaa gacatctctc ctgtcactca agcggctgaa gaaatcgaac
     6841 tgcatccact ggtcgctgca gagaacgata cctttgatat ttacgccgaa ccttttgatc
     6901 caataccgga cccagtgcag catagcgtga cgcagtctta tctcacgagt acacctaaca
     6961 cactttctca gtcatggggg aataccacag ttcctctctc catacccagc gactggtttg
     7021 tacagagcgg accagacatt actttcccca ccgccagcat gggcacccct ttctcccccg
     7081 taacccctgc actgccaact ggaccagttt tcatcacagg aagcgatttt tatcttcacc
     7141 ccacgtggta tttcgccagg cgaaggcgca aacggattcc cctgtttttc accgacgtgg
     7201 cagcctaata gtctaggagc aggtttcccc aatgacacaa aacgtgcaac ttgaaactcc
     7261 gcctggtctt tccaggtcta gaggggtaac actttgtact gcgtttggct ccacgctcga
     7321 tccactggcg agtgttagta acagcactgt tgcttcgtag cggagcatga cggccgtggg
     7381 aactcctcct tggtaacaag gacccacggg gccaaaagcc acgcccacac gggcccgtca
     7441 tgtgtgcaac cccagcacgg cgactttact gcgaaaccca ctttaaagtg acattgaaac
     7501 tggtacccac acactggtga caggctaagg atgcccttca ggtaccccga ggtaacacgc
     7561 gacactcggg atctgagaag gggactgggg cttctataaa agcgctcggt ttaaaaagct
     7621 tctatgcctg aataggtgac cggaggtcgg cacctttcct ttgcaattac tgaccctatg
     7681 aatacactga ctgtttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     7741 ctcactatag gagggccacc atgattgaac aagatggatt gcacgcaggt tctccggccg
     7801 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     7861 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     7921 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     7981 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     8041 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     8101 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     8161 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     8221 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     8281 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac acatggcgat gcctgcttgc
     8341 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     8401 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     8461 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     8521 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     8581 cgaccaagcg aattcgctag gattatccct aatacctgcc accccactct taatcagtgg
     8641 tggaagaacg gtctcagaac tgtttgtttc aattggccat ttaagtttag tagtaaaaga
     8701 ctggttaatg ataacaatgc atcgtaaaac cttcagaagg aaaggagaat gttttgtgga
     8761 ccactttggt tttctttttt gcgtgtggca gttttaagtt attagttttt aaaatcagta
     8821 ctttttaatg gaaacaactt gaccaaaaat ttgtcacaga attttgagac ccattaaaaa
     8881 agttaaatga gaaacctgtg tgttcctttg gtcaacaccg agacatttag gtgaaagaca
     8941 tctaattctg gttttacgaa tctggaaact tcttgaaaat gtaattcttg agttaacact
     9001 tctgggtgga gaatagggtt gttttccccc cacataattg gaaggggaag gaatatcatt
     9061 taaagctatg ggagggttgc tttgattaca acactggaga gaaatgcagc atgttgctga
     9121 ttgcctgtca ctaaaacagg ccaaaaactg agtccttggg ttgcatagaa agctg
//
