LOCUS       sequence-81724-e        9229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV34 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV34 L1L2
FEATURES             Location/Qualifiers
     source          1..9229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     polyA_signal    3119..3240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3130..3149)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3184..3203
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3428..4016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3917..3936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4096..4399
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4514..5819
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4856..5787
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     misc_feature    7272..7716
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7749..7795
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7787..7805
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7815..8609
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7815..8609
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7869..7888)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8479..8498
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8656..9228
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg tggaggccca ccgaggccaa ggtgtacctg
     1621 ccccccgtga gcgtgagcaa ggtggtgagc accgaggagt acgtgaccag gaccaacatc
     1681 tactactacg ccggcagcac caggctgctg gccgtgggcc acccctacta ccccatcaag
     1741 gacaccaacg gcaagaggaa gatcgccgtg cccaaggtga gcggcctgca gtacagggtg
     1801 ttcaggatca ggctgcccga ccccaacaag ttcggcttcc ccgacgccag cttctacaac
     1861 cccgacaagg agaggctggt gtgggcctgc gccggcgtgg aggtgggcag gggccagccc
     1921 ctgggcatcg gcaccagcgg caaccccttc atgaacaagc tggaggacac cgagaacgcc
     1981 gccaagtaca tcggcggcaa catcgccgac agcagggagt gcatgagcgt ggactacaag
     2041 cagacccagc tgtgcatcgt gggctgcaag ccccccctgg gcgagcactg gggcaccggc
     2101 accccctgcg gcacccagaa cgccggcgag tgcccccccc tggagctgaa gaacaccacc
     2161 atccaggacg gcgacatgat cgacgtgggc ttcggcgcca tggacttcaa ggccctgcag
     2221 gccaacaaga gcgacgtgcc catcgacatc agcaacacca tctgcaagta ccccgactac
     2281 ctgggcatgg ccgccgaccc ctacggcgac agcatgtggt tctacatcag gagggagcag
     2341 atgttcgtga ggcacctgtt caacagggcc ggcaccgtgg gcgacgccat ccccgacgac
     2401 ctgatgatca agggcaccgg caacaccgcc agccccagca gctgcgtgtt ctaccccacc
     2461 cccagcggca gcatggtgag cagcgacgcc cagatcttca acaagcccta ctggctgcag
     2521 aaggcccagg gccagaacaa cggcatctgc tggcacaacc agctgttcct gaccgtggtg
     2581 gacaccacca ggagcaccaa cttcagcgtg tgcgtgggca cccagagcac cagcaccacc
     2641 gccccctacg ccaacagcaa cttcaaggag tacctgaggc acgccgagga gtacgacctg
     2701 cagttcgtgt tccagctgtg caagatcaac ctgaccaccg acgtgatgac ctacatccac
     2761 agcatgagca gcagcatcct ggagcagtgg aacttcggcc tgaccccccc ccccagcggc
     2821 accctggagg agacctacag gtacgtgacc agccaggcca tcacctgcca gaggccccag
     2881 ccccccaagg agaccgagga cccctacggc aagatgacct tctgggaggt ggacctgaag
     2941 gagaagttca gcgccgagct ggaccagttc gccctgggca ggaagttcct gctgcagctg
     3001 ggcatgaggg ccaggcccag gctgcaggcc agcaagagga gcgcccccag cagcagcagc
     3061 accgccccca agaagaagag ggccaagagg atctaatagt ctagctggcc agacatgata
     3121 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     3181 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     3241 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     3301 taaagcaagt aaaacctcta caaatgtggt atggaaatgt taattaacta gccatgacca
     3361 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3421 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3481 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3541 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3601 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3661 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3721 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3781 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3841 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3901 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3961 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4021 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4081 taattaacct gcaggcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     4141 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     4201 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     4261 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     4321 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     4381 agtcatcgct attaccatga tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     4441 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     4501 actagtggag ccgagagtaa ttcatacaaa aggagggatc gccttcgcaa ggggagagcc
     4561 cagggaccgt ccctaaattc tcacagaccc aaatccctgt agccgcccca cgacagcgcg
     4621 aggagcatgc gcccagggct gagcgcgggt agatcagagc acacaagctc acagtccccg
     4681 gcggtggggg gaggggcgcg ctgagcgggg gccagggagc tggcgcgggg caaactggga
     4741 aagtggtgtc gtgtgctggc tccgccctct tcccgagggt gggggagaac ggtatataag
     4801 tgcggtagtc gccttggacg ttctttttcg caacgggttt gccgtcagaa cgcaggtgag
     4861 tggcgggtgt ggcttccgcg ggccccggag ctggagccct gctctgagcg ggccgggctg
     4921 atatgcgagt gtcgtccgca gggtttagct gtgagcattc ccacttcgag tggcgggcgg
     4981 tgcgggggtg agagtgcgag gcctagcggc aaccccgtag cctcgcctcg tgtccggctt
     5041 gaggcctagc gtggtgtccg ccgccgcgtg ccactccggc cgcactatgc gttttttgtc
     5101 cttgctgccc tcgattgcct tccagcagca tgggctaaca aagggagggt gtggggctca
     5161 ctcttaagga gcccatgaag cttacgttgg ataggaatgg aagggcagga ggggcgactg
     5221 gggcccgccc gccttcggag cacatgtccg acgccacctg gatggggcga ggcctgtggc
     5281 tttccgaagc aatcgggcgt gagtttagcc tacctgggcc atgtggccct agcactgggc
     5341 acggtctggc ctggcggtgc cgcgttccct tgcctcccaa caagggtgag gccgtcccgc
     5401 ccggcaccag ttgcttgcgc ggaaagatgg ccgctcccgg ggccctgttg caaggagctc
     5461 aaaatggagg acgcggcagc ccggtggagc gggcgggtga gtcacccaca caaaggaaga
     5521 gggccttgcc cctcgccggc cgctgcttcc tgtgaccccg tggtctatcg gccgcatagt
     5581 cacctcgggc ttctcttgag caccgctcgt cgcggcgggg ggaggggatc taatggcgtt
     5641 ggagtttgtt cacatttggt gggtggagac tagtcaggcc agcctggcgc tggaagtcat
     5701 tcttggaatt tgcccctttg agtttggagc gaggctaatt ctcaagcctc ttagcggttc
     5761 aaaggtattt tctaaacccg tttccaggtg ttgtgaaagc caccgctaat tcaaagcaat
     5821 ccggagtata cggatccgcc accatgcgga ggaagaggga cacccacatc aggaggaaga
     5881 gggccagcgc cacccagctg tacaagacct gcaagcagag cggcacctgc ccccccgaca
     5941 tcatccccaa ggtggagggc aacaccctgg ccgaccagat cctgaagtac ggcagcatcg
     6001 gcgtgttctt cggcggcctg ggcatcggca gcggcagcgg caccggcggc aggaccggct
     6061 acgtgcccct gcccaccacc acccccagca ggcccgtgga gatccccctg cagcccacca
     6121 ggccccccgt gatcaccagc gtgggcgcca gcgacagctc catcgtgagc ctggtggagg
     6181 agagcagctt catcgaggcc ggcgtgcccg gccccaccag catcgtgccc agcagcagcg
     6241 gcttcaacgt gaccaccagc gtggacagca cccccgccat catcgatgtg gccaccatca
     6301 gcgacaccac ccaggtgagc gtgagcacct tcaacaaccc caccttcacc gaccccagcg
     6361 tgctgcagcc ccctcccccc ctggaggcca gcggcaggct gctgttcagc aacgacaccg
     6421 tgaccaccca cagctacgag aacatccccc tggacacctt cgtggtgacc accgacaaca
     6481 acagcatcgt gagcagcacc cccatccccg gcaggcaccc ccccgccagg ctgggcctgt
     6541 acggcagggc catccagcag gtgaaggtgg tggaccctgc cttcgtgacc acccccacca
     6601 ggctggtgac ctacgacaac cccgccttcg agggcctgca ggacaccacc ctggagttcc
     6661 agcacagcga cctgcacaac gcccccgaca gcgacttcct ggacatcgtg aagctgcaca
     6721 ggcccgccct gaccgccagg aagaccggca tcagggtgag caggctgggc cagagggcca
     6781 ccatgttcac caggagcggc aagaggatcg gcggcagggt gcacttctac cacgacctga
     6841 gccccatccc caccgagaac atcgagctgc agcccctgct gcccagcgcc agcgccaccg
     6901 tgaccgacgc caacggcatc aacgacggcc tgtacgacgt gctgctggac aacaacgtgg
     6961 acatcaccga ggtggagacc cccaccggca ccaacaccca gagcgtgttc gccagcgaga
     7021 tcagcaccac caccgccaac accaccatcc ccctgaacgc cggcctggac acccaccccg
     7081 gccccgacat cgccctgccc gtgcccaccg ccgagaccat cttcaccccc accgtgcccg
     7141 tgcagcccag cggccccatc tacatctacg gcagcgactt catcctgcac cccagcctgt
     7201 acgtgatccc caggaagagg aagaggctga gctacttctt cgccgacgtg gccacctact
     7261 aatagtctag gagcaggttt ccccaatgac acaaaacgtg caacttgaaa ctccgcctgg
     7321 tctttccagg tctagagggg taacactttg tactgcgttt ggctccacgc tcgatccact
     7381 ggcgagtgtt agtaacagca ctgttgcttc gtagcggagc atgacggccg tgggaactcc
     7441 tccttggtaa caaggaccca cggggccaaa agccacgccc acacgggccc gtcatgtgtg
     7501 caaccccagc acggcgactt tactgcgaaa cccactttaa agtgacattg aaactggtac
     7561 ccacacactg gtgacaggct aaggatgccc ttcaggtacc ccgaggtaac acgcgacact
     7621 cgggatctga gaaggggact ggggcttcta taaaagcgct cggtttaaaa agcttctatg
     7681 cctgaatagg tgaccggagg tcggcacctt tcctttgcaa ttactgaccc tatgaataca
     7741 ctgactgttt gacaattaat catcggcata gtatatcggc atagtataat acgactcact
     7801 ataggagggc caccatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     7861 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     7921 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     7981 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     8041 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     8101 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     8161 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     8221 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     8281 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     8341 cgagcatgcc cgacggcgag gatctcgtcg tgacacatgg cgatgcctgc ttgccgaata
     8401 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     8461 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     8521 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     8581 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     8641 agcgaattcg ctaggattat ccctaatacc tgccacccca ctcttaatca gtggtggaag
     8701 aacggtctca gaactgtttg tttcaattgg ccatttaagt ttagtagtaa aagactggtt
     8761 aatgataaca atgcatcgta aaaccttcag aaggaaagga gaatgttttg tggaccactt
     8821 tggttttctt ttttgcgtgt ggcagtttta agttattagt ttttaaaatc agtacttttt
     8881 aatggaaaca acttgaccaa aaatttgtca cagaattttg agacccatta aaaaagttaa
     8941 atgagaaacc tgtgtgttcc tttggtcaac accgagacat ttaggtgaaa gacatctaat
     9001 tctggtttta cgaatctgga aacttcttga aaatgtaatt cttgagttaa cacttctggg
     9061 tggagaatag ggttgttttc cccccacata attggaaggg gaaggaatat catttaaagc
     9121 tatgggaggg ttgctttgat tacaacactg gagagaaatg cagcatgttg ctgattgcct
     9181 gtcactaaaa caggccaaaa actgagtcct tgggttgcat agaaagctg
//
