LOCUS       sequence-81725-e        9214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV18 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV18 L1L2
FEATURES             Location/Qualifiers
     source          1..9214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     regulatory      1582..1591
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3134..3255
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3145..3164)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3199..3218
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3443..4031
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3932..3951
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4111..4414
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4529..5834
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4871..5802
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     regulatory      5853..5862
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7257..7701
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7734..7780
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7772..7790
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7800..8594
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7800..8594
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7854..7873)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8464..8483
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8641..9213
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg gccctgtgga ggcccagcga caacaccgtg
     1621 tacctgcccc cccccagcgt ggccagggtg gtgaacaccg acgactacgt gaccaggacc
     1681 agcatcttct accacgccgg cagcagcagg ctgctgaccg tgggcaaccc ctacttcagg
     1741 gtgcccgccg gcggcggcaa caagcaggac atccccaagg tgagcgccta ccagtacagg
     1801 gtgttcaggg tgcagctgcc cgaccccaac aagttcggcc tgcccgacaa cagcatctac
     1861 aaccccgaga cccagaggct ggtgtgggcc tgcgccggcg tggagatcgg caggggccag
     1921 cccctgggcg tgggcctgag cggccacccc ttctacaaca agctggacga caccgagagc
     1981 agccacgccg ccaccagcaa cgtgagcgag gacgtgaggg acaacgtgag cgtggactac
     2041 aagcagaccc agctgtgcat cctgggctgc gcccccgcca tcggcgagca ctgggccaag
     2101 ggcaccgcct gcaagagcag gcccctgagc cagggcgact gcccccccct ggagctgaag
     2161 aacaccgtgc tggaggacgg cgacatggtg gacaccggct acggcgccat ggacttcagc
     2221 accctgcagg acaccaagtg cgaggtgccc ctggacatct gccagagcat ctgcaagtac
     2281 cccgactacc tgcagatgag cgccgacccc tacggcgaca gcatgttctt ctgcctgagg
     2341 agggagcagc tgttcgccag gcacttctgg aacagggccg gcaccatggg cgacaccgtg
     2401 ccccagagcc tgtacatcaa gggcaccggc atgagggcca gccccggcag ctgcgtgtac
     2461 agccccagcc ccagcggcag catcgtgacc agcgacagcc agctgttcaa caagccctac
     2521 tggctgcaca aggcccaggg ccacaacaac ggcatctgct ggcacaacca gctgttcgtg
     2581 accgtggtgg acaccaccag gagcaccaac ctgaccatct gcgccagcac ccagagcccc
     2641 gtgcccggcc agtacgacgc caccaagttc aagcagtaca gcaggcacgt ggaggagtac
     2701 gacctgcagt tcatcttcca gctgtgcacc atcaccctga ccgccgacgt gatgagctac
     2761 atccacagca tgaacagcag catcctggag gactggaact tcggcgtgcc cccccccccc
     2821 accaccagcc tggtggacac ctacaggttc gtgcagagcg tggccatcac ctgccagaag
     2881 gacgccgccc ccgccgagaa caaggacccc tacgacaccc tgaagttctg gaacgtggac
     2941 ctgaaggaga agttcagcct ggacctggac cagtaccccc tgggcaggaa gttcctggtg
     3001 caagccggcc tgaggaggaa gcccaccatc ggccccagga agaggagcgc ccccagcgcc
     3061 accaccagca gcaagcccgc caagagggtg agggtgaggg ccaggaagta atagtctagc
     3121 tggccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     3181 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     3241 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     3301 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatgga aatgttaatt
     3361 aactagccat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3421 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3481 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3541 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3601 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3661 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3721 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3781 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3841 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3901 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3961 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4021 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4081 ttgctcacat gttcttaatt aacctgcagg cgttacataa cttacggtaa atggcccgcc
     4141 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     4201 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     4261 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     4321 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     4381 gtacatctac gtattagtca tcgctattac catgatgatg cggttttggc agtacatcaa
     4441 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     4501 tgggagtttg ttttgactag tggagccgag agtaattcat acaaaaggag ggatcgcctt
     4561 cgcaagggga gagcccaggg accgtcccta aattctcaca gacccaaatc cctgtagccg
     4621 ccccacgaca gcgcgaggag catgcgccca gggctgagcg cgggtagatc agagcacaca
     4681 agctcacagt ccccggcggt ggggggaggg gcgcgctgag cgggggccag ggagctggcg
     4741 cggggcaaac tgggaaagtg gtgtcgtgtg ctggctccgc cctcttcccg agggtggggg
     4801 agaacggtat ataagtgcgg tagtcgcctt ggacgttctt tttcgcaacg ggtttgccgt
     4861 cagaacgcag gtgagtggcg ggtgtggctt ccgcgggccc cggagctgga gccctgctct
     4921 gagcgggccg ggctgatatg cgagtgtcgt ccgcagggtt tagctgtgag cattcccact
     4981 tcgagtggcg ggcggtgcgg gggtgagagt gcgaggccta gcggcaaccc cgtagcctcg
     5041 cctcgtgtcc ggcttgaggc ctagcgtggt gtccgccgcc gcgtgccact ccggccgcac
     5101 tatgcgtttt ttgtccttgc tgccctcgat tgccttccag cagcatgggc taacaaaggg
     5161 agggtgtggg gctcactctt aaggagccca tgaagcttac gttggatagg aatggaaggg
     5221 caggaggggc gactggggcc cgcccgcctt cggagcacat gtccgacgcc acctggatgg
     5281 ggcgaggcct gtggctttcc gaagcaatcg ggcgtgagtt tagcctacct gggccatgtg
     5341 gccctagcac tgggcacggt ctggcctggc ggtgccgcgt tcccttgcct cccaacaagg
     5401 gtgaggccgt cccgcccggc accagttgct tgcgcggaaa gatggccgct cccggggccc
     5461 tgttgcaagg agctcaaaat ggaggacgcg gcagcccggt ggagcgggcg ggtgagtcac
     5521 ccacacaaag gaagagggcc ttgcccctcg ccggccgctg cttcctgtga ccccgtggtc
     5581 tatcggccgc atagtcacct cgggcttctc ttgagcaccg ctcgtcgcgg cggggggagg
     5641 ggatctaatg gcgttggagt ttgttcacat ttggtgggtg gagactagtc aggccagcct
     5701 ggcgctggaa gtcattcttg gaatttgccc ctttgagttt ggagcgaggc taattctcaa
     5761 gcctcttagc ggttcaaagg tattttctaa acccgtttcc aggtgttgtg aaagccaccg
     5821 ctaattcaaa gcaatccgga gtatacggat ccgccaccat ggtgagccac agggccgcca
     5881 ggaggaagag ggccagcgtg accgacctgt acaagacctg caagcagagc ggcacctgcc
     5941 ccagcgacgt ggtgaacaag gtggagggca ccaccctggc cgacaagatc ctgcagtgga
     6001 gcagcctggg catcttcctg ggcggcctgg gcatcggcac cggctccggc accggcggca
     6061 ggaccggcta catccccctg ggcggcagga gcaacaccgt ggtggacgtg ggccccacca
     6121 ggccccccgt ggtgatcgag cccgtgggcc ctaccgatcc cagcatcgtg accctgatcg
     6181 aggacagcag cgtggtgacc agcggcgccc ccaggcccac cttcaccggc accagcggct
     6241 tcgacatcac cagcgccggc accaccaccc ccgccgtgct ggacatcacc cccagcagca
     6301 ccagcgtgag catcagcacc accaacttca ccaatcccgc cttcagcgac cccagcatca
     6361 tcgaggtgcc ccagaccggc gaggtgagcg gcaacgtgtt cgtgggcacc cccaccagcg
     6421 gcacccacgg ctacgaggag atccccctgc agaccttcgc cagcagcggc accggcgagg
     6481 agcccatcag cagcaccccc ctgcccaccg tgaggagggt gaccggcccc aggctgtaca
     6541 gcagggccta ccagcaggtg agcgtggcca accccgagtt cctgaccagg cccagcagcc
     6601 tgatcaccta cgacaacccc gcctttgagc ccatggacac caccctgacc ttcgagccca
     6661 ggagcaacgt gcccgacagc gacttcatgg acatcatcag gctgcacagg cccgctagca
     6721 ccagcaggag gggcaccgtg aggttcagca ggctgggcca gagggccacc atgttcacca
     6781 ggagcggcac ccagatcggc gccagggtgc acttctacca cgacatcagc cccatcgccc
     6841 ccagccccga gtacatcgag ctgcagcccc tggtgagcgc caccgaggac aacggcctgt
     6901 tcgacatcta cgccgacgac atcgaccccg ccctgcccgt gcccagcagg cccaccacca
     6961 gcagcgccgt gagcacctac agccccacca tcagcagcgc cagcagctac agcaacgtga
     7021 ccgtgcccct gaccagcagc tgggacgtgc ccgtgtacac cggccccgac atcaccctgc
     7081 ccagcaccac cagcgtgtgg cccatcgtga gccccaccga ccccgccagc acccagtaca
     7141 tcggcatcca cggcacccac tactacctgt ggcccctgta ctacttcatc cccaagaaga
     7201 ggaagagggt gccctacttc ttcgccgacg gcttcgtggc cgcctaatag tctaggagca
     7261 ggtttcccca atgacacaaa acgtgcaact tgaaactccg cctggtcttt ccaggtctag
     7321 aggggtaaca ctttgtactg cgtttggctc cacgctcgat ccactggcga gtgttagtaa
     7381 cagcactgtt gcttcgtagc ggagcatgac ggccgtggga actcctcctt ggtaacaagg
     7441 acccacgggg ccaaaagcca cgcccacacg ggcccgtcat gtgtgcaacc ccagcacggc
     7501 gactttactg cgaaacccac tttaaagtga cattgaaact ggtacccaca cactggtgac
     7561 aggctaagga tgcccttcag gtaccccgag gtaacacgcg acactcggga tctgagaagg
     7621 ggactggggc ttctataaaa gcgctcggtt taaaaagctt ctatgcctga ataggtgacc
     7681 ggaggtcggc acctttcctt tgcaattact gaccctatga atacactgac tgtttgacaa
     7741 ttaatcatcg gcatagtata tcggcatagt ataatacgac tcactatagg agggccacca
     7801 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     7861 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     7921 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     7981 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     8041 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     8101 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     8161 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     8221 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     8281 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     8341 gcgaggatct cgtcgtgaca catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     8401 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     8461 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     8521 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     8581 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga attcgctagg
     8641 attatcccta atacctgcca ccccactctt aatcagtggt ggaagaacgg tctcagaact
     8701 gtttgtttca attggccatt taagtttagt agtaaaagac tggttaatga taacaatgca
     8761 tcgtaaaacc ttcagaagga aaggagaatg ttttgtggac cactttggtt ttcttttttg
     8821 cgtgtggcag ttttaagtta ttagttttta aaatcagtac tttttaatgg aaacaacttg
     8881 accaaaaatt tgtcacagaa ttttgagacc cattaaaaaa gttaaatgag aaacctgtgt
     8941 gttcctttgg tcaacaccga gacatttagg tgaaagacat ctaattctgg ttttacgaat
     9001 ctggaaactt cttgaaaatg taattcttga gttaacactt ctgggtggag aatagggttg
     9061 ttttcccccc acataattgg aaggggaagg aatatcattt aaagctatgg gagggttgct
     9121 ttgattacaa cactggagag aaatgcagca tgttgctgat tgcctgtcac taaaacaggc
     9181 caaaaactga gtccttgggt tgcatagaaa gctg
//
