LOCUS       sequence-81726-e        9223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV40 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV40 L1L2
FEATURES             Location/Qualifiers
     source          1..9223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     polyA_signal    3128..3249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3139..3158)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3193..3212
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3437..4025
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3926..3945
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4105..4408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4523..5828
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4865..5796
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     regulatory      5847..5856
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7266..7710
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7743..7789
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7781..7799
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7809..8603
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7809..8603
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7863..7882)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8473..8492
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8650..9222
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg tggcagctga acgagaacca ggtgtacctg
     1621 ccccccccca cccccgtggc caccatcgtg agcaccgacg agtacgtgca gaggaccagc
     1681 ctgtactacc acgccggcag cgccaggctg ctgaccatcg ggcaccccta cttcgagctg
     1741 aagaagccca acggcgacat cagcgtgccc aaggtgtccg gccaccagta cagggtgttc
     1801 agggtgaggc tgcccgaccc caacaagttc ggcctgagcg acaccagcct gttcaacagc
     1861 gagacccaga ggctggtgtg ggcctgcgtg ggcgtggagg tgggcagggg ccagcccctg
     1921 ggcgtgggcg tgagcggcca cccctacttc aacaaggacg aggacgtgga gaacagcagc
     1981 gcctacggca ccggccccgg ccaggacagc agggagaacg tggccatgga ctacaagcag
     2041 acccagctgt gcatgctggg ctgcaccccc cccatcggcg agtactgggg caagggcacc
     2101 ccctgcaacg ccagcagggt gaccctgggc gactgccccg tgctggagct gaagaccgag
     2161 gtgatccagg acggcgacat ggtggacacc ggcttcggcg ccatggactt cgccagcctg
     2221 caggccaaca agagcgacgt gcccctggac ctgtgcacca gcatcagcaa gtaccccgac
     2281 tacctgggca tggccgccga gccctacggc aacagcctgt tcttcttcct gaggagggag
     2341 cagatgttcg tgaggcactt cttcaacagg gccggcacca ccggcgacag cgtgcccacc
     2401 gacctgtaca tcaccggcac cagcggcagg acccccatcg ccggcagcat ctactacagc
     2461 acccccagcg gcagcctggt gaccagcgac agccagatct tcaacaagcc cctgtggatt
     2521 cagaaggccc agggccacaa caacggcatc tgcttcggca accagctgtt cgtgaccgtg
     2581 gtggacacca ccaggagcac caacctgacc ctgtgcgccg ccacccagag ccccaccccc
     2641 accccctaca acaacagcaa cttcaaggag tacctgaggc acggcgagga gttcgacctg
     2701 cagttcatct tccagctgtg cgtgatcacc ctgaacgccg aggtgatgac ctacatccac
     2761 gccatggacc ccaccctgct ggaggactgg aacttcaaga tcgccccccc cgccagcgcc
     2821 agcctggagg acacctacag gttcctgacc aacaaggcca tcgcctgcca gagggacgcc
     2881 ccccccaagg tgagggagga cccctacaag aagtacaagt tctgggacgt gaacctgacc
     2941 gagaggttca gcagccagct ggaccagttc cccctgggca ggaagttcct gatgcaggcc
     3001 ggcgtgaggg ccggccccag gttcaagagc aggaagaggc ccgcccccag ctccagcagc
     3061 agcagcaagc ccgtgacccc caagaggaag aagaccaaga ggtaatagtc tagctggcca
     3121 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     3181 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     3241 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     3301 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggaaatgtt aattaactag
     3361 ccatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3421 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3481 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3541 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3601 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3661 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3721 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3781 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3841 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3901 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3961 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4021 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4081 acatgttctt aattaacctg caggcgttac ataacttacg gtaaatggcc cgcctggctg
     4141 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     4201 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     4261 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     4321 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     4381 ctacgtatta gtcatcgcta ttaccatgat gatgcggttt tggcagtaca tcaatgggcg
     4441 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     4501 tttgttttga ctagtggagc cgagagtaat tcatacaaaa ggagggatcg ccttcgcaag
     4561 gggagagccc agggaccgtc cctaaattct cacagaccca aatccctgta gccgccccac
     4621 gacagcgcga ggagcatgcg cccagggctg agcgcgggta gatcagagca cacaagctca
     4681 cagtccccgg cggtgggggg aggggcgcgc tgagcggggg ccagggagct ggcgcggggc
     4741 aaactgggaa agtggtgtcg tgtgctggct ccgccctctt cccgagggtg ggggagaacg
     4801 gtatataagt gcggtagtcg ccttggacgt tctttttcgc aacgggtttg ccgtcagaac
     4861 gcaggtgagt ggcgggtgtg gcttccgcgg gccccggagc tggagccctg ctctgagcgg
     4921 gccgggctga tatgcgagtg tcgtccgcag ggtttagctg tgagcattcc cacttcgagt
     4981 ggcgggcggt gcgggggtga gagtgcgagg cctagcggca accccgtagc ctcgcctcgt
     5041 gtccggcttg aggcctagcg tggtgtccgc cgccgcgtgc cactccggcc gcactatgcg
     5101 ttttttgtcc ttgctgccct cgattgcctt ccagcagcat gggctaacaa agggagggtg
     5161 tggggctcac tcttaaggag cccatgaagc ttacgttgga taggaatgga agggcaggag
     5221 gggcgactgg ggcccgcccg ccttcggagc acatgtccga cgccacctgg atggggcgag
     5281 gcctgtggct ttccgaagca atcgggcgtg agtttagcct acctgggcca tgtggcccta
     5341 gcactgggca cggtctggcc tggcggtgcc gcgttccctt gcctcccaac aagggtgagg
     5401 ccgtcccgcc cggcaccagt tgcttgcgcg gaaagatggc cgctcccggg gccctgttgc
     5461 aaggagctca aaatggagga cgcggcagcc cggtggagcg ggcgggtgag tcacccacac
     5521 aaaggaagag ggccttgccc ctcgccggcc gctgcttcct gtgaccccgt ggtctatcgg
     5581 ccgcatagtc acctcgggct tctcttgagc accgctcgtc gcggcggggg gaggggatct
     5641 aatggcgttg gagtttgttc acatttggtg ggtggagact agtcaggcca gcctggcgct
     5701 ggaagtcatt cttggaattt gcccctttga gtttggagcg aggctaattc tcaagcctct
     5761 tagcggttca aaggtatttt ctaaacccgt ttccaggtgt tgtgaaagcc accgctaatt
     5821 caaagcaatc cggagtatac ggatccgcca ccatggtgag cagcaggccc aggaggagga
     5881 agagggccag cgccacccag ctgtaccaga cctgcaaggc cgccggcacc tgcccccccg
     5941 acgtggtgca caaggtggag cagaccaccg tggccgacca gatcctgaag tggggcagca
     6001 tgggcgtgtt cttcggcggc ctgggcatcg gcagcggcag cggcaccggc ggcagggccg
     6061 gctacgtgcc cctgagcacc ggcagcaggg ccgtgccccc caagagcctg gtgcccgacg
     6121 tggtggccag gccccccgtg gtggtggaca ccgtggcccc cagcgacccc agcatcgtga
     6181 gcctgatcga ggagagcagc atcatccaga gcggcgcccc cagcctgacc atccccaccg
     6241 agggcggctt cagcgtgacc agcagcggca ccgacgtgcc cgccatcctg gacgtgagca
     6301 gcaccaacac cgtgcacgtg accgccacca cccaccacaa tcccgtgttc accgacccca
     6361 gcgtggtgca gcccatcccc cccgtggagg ccggcggcag gctgatcgtg agccacagca
     6421 ccatcaccac cagcgccgcc gaggagatcc ccctggacac cttcgtggtg cacagcgacc
     6481 ccctgagcag cacccccgtg cccggcacca gcggcaggcc caggctgggc ctgtacagca
     6541 aggccctgca gcaggtggag atcgtggacc ccgccttcct gagcaccccc cagaggctga
     6601 tcacctacga caaccccgtg ttcgagaacg tggacgacac cctgcagttc gagcagccca
     6661 gcatccacga cgcccccgac cccgccttca tggacatcat caccctgcac aggcccgccc
     6721 tgaccagcag gaggggcgtg atcaggttca gcagggtggg ccagaggggc accatgtaca
     6781 ccaggagggg caccaggatc ggcggcaggg tgcacttctt cagggacatc agccccatcg
     6841 gcgccgccga cgacatcgag ctgcaccccc tggtggccag cgcccctcac acactcgaga
     6901 ccccccacac cctggagacc cccctggaca ccaccgacgc cctgttcgac gtgtacgccg
     6961 acatggacac catcgacgac gacgccgcct acgccacctt cagcctccac cccgccgaca
     7021 gcaccaggat cagcaacacc agcatccccc tggccaccgt gagcgacacc ctgctgacca
     7081 gcggccccga catcgtgttc ccttccatcc ccgccggcac cccctacctg cccgtgagcc
     7141 ccagcatccc cgccatcagc gtgctgatcc acggcaccga ctactacctg caccccgcct
     7201 actacctgag gaagaggagg aagaggattc tggcccacca gtacgtggcc acctaatagt
     7261 ctaggagcag gtttccccaa tgacacaaaa cgtgcaactt gaaactccgc ctggtctttc
     7321 caggtctaga ggggtaacac tttgtactgc gtttggctcc acgctcgatc cactggcgag
     7381 tgttagtaac agcactgttg cttcgtagcg gagcatgacg gccgtgggaa ctcctccttg
     7441 gtaacaagga cccacggggc caaaagccac gcccacacgg gcccgtcatg tgtgcaaccc
     7501 cagcacggcg actttactgc gaaacccact ttaaagtgac attgaaactg gtacccacac
     7561 actggtgaca ggctaaggat gcccttcagg taccccgagg taacacgcga cactcgggat
     7621 ctgagaaggg gactggggct tctataaaag cgctcggttt aaaaagcttc tatgcctgaa
     7681 taggtgaccg gaggtcggca cctttccttt gcaattactg accctatgaa tacactgact
     7741 gtttgacaat taatcatcgg catagtatat cggcatagta taatacgact cactatagga
     7801 gggccaccat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     7861 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     7921 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     7981 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     8041 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     8101 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     8161 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     8221 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     8281 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     8341 tgcccgacgg cgaggatctc gtcgtgacac atggcgatgc ctgcttgccg aatatcatgg
     8401 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     8461 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     8521 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     8581 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgaa
     8641 ttcgctagga ttatccctaa tacctgccac cccactctta atcagtggtg gaagaacggt
     8701 ctcagaactg tttgtttcaa ttggccattt aagtttagta gtaaaagact ggttaatgat
     8761 aacaatgcat cgtaaaacct tcagaaggaa aggagaatgt tttgtggacc actttggttt
     8821 tcttttttgc gtgtggcagt tttaagttat tagtttttaa aatcagtact ttttaatgga
     8881 aacaacttga ccaaaaattt gtcacagaat tttgagaccc attaaaaaag ttaaatgaga
     8941 aacctgtgtg ttcctttggt caacaccgag acatttaggt gaaagacatc taattctggt
     9001 tttacgaatc tggaaacttc ttgaaaatgt aattcttgag ttaacacttc tgggtggaga
     9061 atagggttgt tttcccccca cataattgga aggggaagga atatcattta aagctatggg
     9121 agggttgctt tgattacaac actggagaga aatgcagcat gttgctgatt gcctgtcact
     9181 aaaacaggcc aaaaactgag tccttgggtt gcatagaaag ctg
//
