LOCUS       sequence-81727-e        9418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV38 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV38 L1L2
FEATURES             Location/Qualifiers
     source          1..9418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     polyA_signal    3143..3264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3154..3173)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3208..3227
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3452..4040
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3941..3960
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4120..4423
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4538..5843
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4880..5811
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     regulatory      5862..5871
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7461..7905
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7938..7984
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7976..7994
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8004..8798
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8004..8798
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8058..8077)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8668..8687
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8845..9417
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg accctgtggc tgcccgccag cggcaagatc
     1621 tacctgcccc ccaccccccc cgtggccagg gtgcagagca ccgacgagta cgtggagagg
     1681 accgacatct actaccacgc caccagcgac aggctgctga ccgtgggcca cccctacttc
     1741 gacgtgagga gccaggacgg ccagaagatc gaggtgccca aggtgagcgg caaccagtac
     1801 aggagcttca gggtgacctt ccccgacccc aacaagttcg ccctggccga catgagcgtg
     1861 tacgaccccg acaagtacag gctggtgtgg gcctgcaagg gcctggagat cggcaggggc
     1921 cagcccctgg gcgtgggcac caccggccac cccctgttca acaaggtgag ggacaccgag
     1981 aacagcagca actaccagaa caccagcacc gacgacaggc agaacaccag cttcgacccc
     2041 aagcaggtgc agatgttcat catcggctgc accccctgcc tgggcgagta ctgggacaag
     2101 gcccccgtgt gcgacaacgc cggcgaccag accggcctgt gcccccccct ggagctgaag
     2161 aacagcgtga tcgaggacgg cgacatgttc gacatcggct tcggcaacat caacaacaag
     2221 accctgagct tcaacaggag cgacgtgagc ctggacatcg tgaacgagac ctgcaagtac
     2281 cccgacttcc tgaccatgag caacgacgtg tacggcgaca gctgcttctt cttcgtgagg
     2341 agggagcagt gctacgccag gcactacttc gtgaggggcg gcgccgtggg cgacgccatc
     2401 cccgacggca ccgtgaacca gaaccacaac tactacctgc ccgccaagaa cggccagggc
     2461 cagaggaccc tgggcaacag cacctacttc cccaccgtga gcggcagcct ggtgaccagc
     2521 gacgcccagc tgttcaacag gcccttctgg ctgcagaggg cccagggcca caacaacggc
     2581 atcctgtggg gcaaccagat gttcgtgacc gtggccgaca acaccaggaa caccaacttc
     2641 accatcagcg tgagcaccga gaacggcggc gcccaggagt acgacagcgc caacatcagg
     2701 gagtacctga ggcacgtgga ggagtaccag ctgagcttca tcctgcagct gtgcaaggtg
     2761 cccctgaacg ccgaggtgct gacccagatc aacgccatga acagcggcat cctggagaac
     2821 tggcagctgg gcttcgtgcc cacccccgac aacagcgtgc acgacaccta caggtacatc
     2881 accagcaagg ccaccaagtg ccccgacgcc gtgcccgaga ccgagaagga ggaccccttc
     2941 ggccagtaca ccttctggaa cgtggacatg accgagaagc tgagcctgga cctggaccag
     3001 taccccctgg gcaggaagtt cctgttccag gccggcctgc agaccgccag gaccagggcc
     3061 gtgaagaggc ccctggtgag gaagagcagc aagagcgtga agaggaagag gacccagtaa
     3121 tagtctagct ggccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     3181 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     3241 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     3301 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggaa
     3361 atgttaatta actagccatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3421 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3481 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3541 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3601 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3661 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3721 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3781 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3841 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3901 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3961 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4021 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4081 gctggccttt tgctcacatg ttcttaatta acctgcaggc gttacataac ttacggtaaa
     4141 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     4201 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     4261 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     4321 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     4381 tacttggcag tacatctacg tattagtcat cgctattacc atgatgatgc ggttttggca
     4441 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     4501 tgacgtcaat gggagtttgt tttgactagt ggagccgaga gtaattcata caaaaggagg
     4561 gatcgccttc gcaaggggag agcccaggga ccgtccctaa attctcacag acccaaatcc
     4621 ctgtagccgc cccacgacag cgcgaggagc atgcgcccag ggctgagcgc gggtagatca
     4681 gagcacacaa gctcacagtc cccggcggtg gggggagggg cgcgctgagc gggggccagg
     4741 gagctggcgc ggggcaaact gggaaagtgg tgtcgtgtgc tggctccgcc ctcttcccga
     4801 gggtggggga gaacggtata taagtgcggt agtcgccttg gacgttcttt ttcgcaacgg
     4861 gtttgccgtc agaacgcagg tgagtggcgg gtgtggcttc cgcgggcccc ggagctggag
     4921 ccctgctctg agcgggccgg gctgatatgc gagtgtcgtc cgcagggttt agctgtgagc
     4981 attcccactt cgagtggcgg gcggtgcggg ggtgagagtg cgaggcctag cggcaacccc
     5041 gtagcctcgc ctcgtgtccg gcttgaggcc tagcgtggtg tccgccgccg cgtgccactc
     5101 cggccgcact atgcgttttt tgtccttgct gccctcgatt gccttccagc agcatgggct
     5161 aacaaaggga gggtgtgggg ctcactctta aggagcccat gaagcttacg ttggatagga
     5221 atggaagggc aggaggggcg actggggccc gcccgccttc ggagcacatg tccgacgcca
     5281 cctggatggg gcgaggcctg tggctttccg aagcaatcgg gcgtgagttt agcctacctg
     5341 ggccatgtgg ccctagcact gggcacggtc tggcctggcg gtgccgcgtt cccttgcctc
     5401 ccaacaaggg tgaggccgtc ccgcccggca ccagttgctt gcgcggaaag atggccgctc
     5461 ccggggccct gttgcaagga gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg
     5521 gtgagtcacc cacacaaagg aagagggcct tgcccctcgc cggccgctgc ttcctgtgac
     5581 cccgtggtct atcggccgca tagtcacctc gggcttctct tgagcaccgc tcgtcgcggc
     5641 ggggggaggg gatctaatgg cgttggagtt tgttcacatt tggtgggtgg agactagtca
     5701 ggccagcctg gcgctggaag tcattcttgg aatttgcccc tttgagtttg gagcgaggct
     5761 aattctcaag cctcttagcg gttcaaaggt attttctaaa cccgtttcca ggtgttgtga
     5821 aagccaccgc taattcaaag caatccggag tatacggatc cgccaccatg gtgagggcca
     5881 ggaggaccaa gagggccagc gtgaccgaca tctacagggg ctgcaaggcc agcaacacct
     5941 gcccccccga cgtgatcaac aaggtggagc agagcaccat cgccgacaag atcctgaagt
     6001 acggcagcgc cgccgtgttc ttcggcggcc tgggcatcag caccggcagg ggcaccggcg
     6061 gcgccaccgg ctacgtgccc ctgggccagg gccccggcgt gagggtgggc ggcgccccta
     6121 ccgtggtgag gcccggcgtg atccccgagg tgatcggccc caccgagctg atccccatcg
     6181 acagcgtgac ccctatcgac cccaccgccc ccagcatcgt gagcctgacc gacagcagcg
     6241 ccgtggacct gctgcccggc gaggtggaga ccatcgccga ggtgcacccc ggccccatcg
     6301 accccatcga gatcgacacc cccgtggtga gcggcggcag gaacaccaac gccatcctgg
     6361 aggtggccga cccccacccc cccaccaggg ccaccgtgag caggacccag tacaacaacc
     6421 ccgccttcca gatcatcagc gaggtgatcc ccaccagcgg cgagagcagc ctggccgacc
     6481 acgtgctggt gagcgagggc agcggcggcc agcagatcgg cggcaccagg accgccgagg
     6541 agatcgagct gcagcccctg ctgagcaggt acagcttcga gatcgaggag cccacccccc
     6601 ccaggaggac cagcaccccc ctgcagaggg ccaggcagca gttcagcagc ctgaggaggg
     6661 ccctgtacaa caggaggctg accgagcagg tgggcgtgac cgaccccctg ttcttcacca
     6721 gccccagcaa gctggtgagg ttccagttcg acaaccccgt gttcgacgag caggtgaccc
     6781 agatcttcga gcaggacatc gccgacttcg aggagccccc cgacaggcag ttcctggacg
     6841 tggtgaagct gggcaggccc accctgaccg agagcgccga gggctacgtg agggtgagca
     6901 ggctgggcag gaggggcacc atcaggacca ggagcggcac ccagatcggc agccaggtgc
     6961 acttctacag ggacctgagc accatcaaca ccgaggagcc cctggagatg cagctgctgg
     7021 gcgagcacag cggcgacgcc agcatcgtgc agggccccgt ggagagcacc ctggtggacg
     7081 tgaacgtgac cgaggtgccc gagggcgtgc tgaccgagac cagcatggac cccgacacct
     7141 tcaacagcga ggacctgctg ctggacgacg ccatcgagga cttcagcggc agccagctgg
     7201 tggtgggcac ccccaggagg agcaccacca gcatcaccgt gcccaggttc cagacccccc
     7261 agaaccccac catctactac caggacatcc agggctacca cgtgagctac cccgagagca
     7321 gggagaggcc cgccatcatc taccccaccc ccgacatccc caccgtggtg atccacgtgg
     7381 ccgacagcag cggcgacttc tacctgcacc ccagcctgag gtggaggagg aggaagagga
     7441 agtacctgta atagtctagg agcaggtttc cccaatgaca caaaacgtgc aacttgaaac
     7501 tccgcctggt ctttccaggt ctagaggggt aacactttgt actgcgtttg gctccacgct
     7561 cgatccactg gcgagtgtta gtaacagcac tgttgcttcg tagcggagca tgacggccgt
     7621 gggaactcct ccttggtaac aaggacccac ggggccaaaa gccacgccca cacgggcccg
     7681 tcatgtgtgc aaccccagca cggcgacttt actgcgaaac ccactttaaa gtgacattga
     7741 aactggtacc cacacactgg tgacaggcta aggatgccct tcaggtaccc cgaggtaaca
     7801 cgcgacactc gggatctgag aaggggactg gggcttctat aaaagcgctc ggtttaaaaa
     7861 gcttctatgc ctgaataggt gaccggaggt cggcaccttt cctttgcaat tactgaccct
     7921 atgaatacac tgactgtttg acaattaatc atcggcatag tatatcggca tagtataata
     7981 cgactcacta taggagggcc accatgattg aacaagatgg attgcacgca ggttctccgg
     8041 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     8101 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     8161 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     8221 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     8281 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     8341 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     8401 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     8461 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     8521 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacacatggc gatgcctgct
     8581 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     8641 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     8701 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     8761 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     8821 gaccgaccaa gcgaattcgc taggattatc cctaatacct gccaccccac tcttaatcag
     8881 tggtggaaga acggtctcag aactgtttgt ttcaattggc catttaagtt tagtagtaaa
     8941 agactggtta atgataacaa tgcatcgtaa aaccttcaga aggaaaggag aatgttttgt
     9001 ggaccacttt ggttttcttt tttgcgtgtg gcagttttaa gttattagtt tttaaaatca
     9061 gtacttttta atggaaacaa cttgaccaaa aatttgtcac agaattttga gacccattaa
     9121 aaaagttaaa tgagaaacct gtgtgttcct ttggtcaaca ccgagacatt taggtgaaag
     9181 acatctaatt ctggttttac gaatctggaa acttcttgaa aatgtaattc ttgagttaac
     9241 acttctgggt ggagaatagg gttgttttcc ccccacataa ttggaagggg aaggaatatc
     9301 atttaaagct atgggagggt tgctttgatt acaacactgg agagaaatgc agcatgttgc
     9361 tgattgcctg tcactaaaac aggccaaaaa ctgagtcctt gggttgcata gaaagctg
//
