LOCUS       sequence-81728-e        9244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV42 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV42 L1L2
FEATURES             Location/Qualifiers
     source          1..9244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     polyA_signal    3119..3240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3130..3149)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3184..3203
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3428..4016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3917..3936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4096..4399
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4514..5819
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4856..5787
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     misc_feature    7287..7731
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7764..7810
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7802..7820
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7830..8624
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7830..8624
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7884..7903)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8494..8513
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8671..9243
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg agcgtgtgga ggcccagcga cgacaaggtg
     1621 tacctgcccc ctccccccgt gagcaaggtg gtgagcaccg acgagtacgt gcagaggacc
     1681 aactacttct accacgccag cagcagcagg ctgctggtgg tgggccaccc ctactactcc
     1741 atcaccaaga ggcccaacaa gaccagcatc cccaaggtga gcggcctgca gtacagggtg
     1801 ttcagggtga ggctgcccga ccccaacaag ttcaccctgc ccgagaccaa cctgtacaac
     1861 cccgacaccc agaggatggt gtgggcctgc gtgggcctgg aggtgggcag gggccagccc
     1921 ctgggcgtgg gcatcagcgg ccaccccctg ctgaacaagc tggacgacac cgagaacgcc
     1981 cccacctacg gcggcggccc cggcaccgac aacagggaga acgtgagcat ggactacaag
     2041 cagacccagc tgtgcctggt gggctgcaag cccgctatcg gcgagcactg gggcaagggc
     2101 accgcctgca ccccccagag caacggcgac tgcccccccc tggagctgaa gaacagcttc
     2161 atccaggacg gcgacatggt ggacgtgggc ttcggggccc tggacttcgg cgccctgcag
     2221 agcagcaagg ccgaggtgcc cctggacatc gtgaacagca tcaccaagta ccccgactac
     2281 ctgaagatga gcgccgaggc ctacggcgac agcatgttct tcttcctgag gagggagcag
     2341 atgttcgtga ggcacctgtt caacagggcc ggcgccatcg gcgagcccgt gcccgacgag
     2401 ctgtacacca aggccgccaa caacgccagc ggcaggcaca acctgggcag cagcatctac
     2461 taccccaccc ccagcggcag catggtgacc agcgacgccc agctgttcaa caagccctac
     2521 tggctgcagc aggcccaggg ccacaacaac ggcatctgct ggggcgacca gctgttcctg
     2581 accgtggtgg acaccaccag gagcaccaac atgaccctgt gcgccaccgc caccagcggc
     2641 gacacctaca ccgccgccaa cttcaaggag tacctgaggc acgccgagga gtacgacgtg
     2701 cagttcatct tccagctgtg caagatcacc ctgaccgtgg aggtgatgag ctacatccac
     2761 aacatgaacc ccaacatcct ggaggagtgg aacgtgggcg tggccccccc ccccagcggc
     2821 accctggagg acagctacag gtacgtgcag agcgaggcca tcaggtgcca ggccaaggtg
     2881 accacccccg agaagaagga cccctacagc gacttcagct tctgggaggt gaacctgagc
     2941 gagaagttca gcaccgacct ggaccagttc cccctgggca ggaagttcct gctgcaggcc
     3001 ggcctgaggg ccaggcccaa gctgagcgtg ggcaagagga aggccagcac cgccaagagc
     3061 gtgagcagcg ccaagaggaa gaagacccac aagtaatagt ctagctggcc agacatgata
     3121 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     3181 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     3241 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     3301 taaagcaagt aaaacctcta caaatgtggt atggaaatgt taattaacta gccatgacca
     3361 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3421 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3481 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3541 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3601 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3661 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3721 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3781 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3841 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3901 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3961 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4021 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4081 taattaacct gcaggcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     4141 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     4201 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     4261 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     4321 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     4381 agtcatcgct attaccatga tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     4441 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     4501 actagtggag ccgagagtaa ttcatacaaa aggagggatc gccttcgcaa ggggagagcc
     4561 cagggaccgt ccctaaattc tcacagaccc aaatccctgt agccgcccca cgacagcgcg
     4621 aggagcatgc gcccagggct gagcgcgggt agatcagagc acacaagctc acagtccccg
     4681 gcggtggggg gaggggcgcg ctgagcgggg gccagggagc tggcgcgggg caaactggga
     4741 aagtggtgtc gtgtgctggc tccgccctct tcccgagggt gggggagaac ggtatataag
     4801 tgcggtagtc gccttggacg ttctttttcg caacgggttt gccgtcagaa cgcaggtgag
     4861 tggcgggtgt ggcttccgcg ggccccggag ctggagccct gctctgagcg ggccgggctg
     4921 atatgcgagt gtcgtccgca gggtttagct gtgagcattc ccacttcgag tggcgggcgg
     4981 tgcgggggtg agagtgcgag gcctagcggc aaccccgtag cctcgcctcg tgtccggctt
     5041 gaggcctagc gtggtgtccg ccgccgcgtg ccactccggc cgcactatgc gttttttgtc
     5101 cttgctgccc tcgattgcct tccagcagca tgggctaaca aagggagggt gtggggctca
     5161 ctcttaagga gcccatgaag cttacgttgg ataggaatgg aagggcagga ggggcgactg
     5221 gggcccgccc gccttcggag cacatgtccg acgccacctg gatggggcga ggcctgtggc
     5281 tttccgaagc aatcgggcgt gagtttagcc tacctgggcc atgtggccct agcactgggc
     5341 acggtctggc ctggcggtgc cgcgttccct tgcctcccaa caagggtgag gccgtcccgc
     5401 ccggcaccag ttgcttgcgc ggaaagatgg ccgctcccgg ggccctgttg caaggagctc
     5461 aaaatggagg acgcggcagc ccggtggagc gggcgggtga gtcacccaca caaaggaaga
     5521 gggccttgcc cctcgccggc cgctgcttcc tgtgaccccg tggtctatcg gccgcatagt
     5581 cacctcgggc ttctcttgag caccgctcgt cgcggcgggg ggaggggatc taatggcgtt
     5641 ggagtttgtt cacatttggt gggtggagac tagtcaggcc agcctggcgc tggaagtcat
     5701 tcttggaatt tgcccctttg agtttggagc gaggctaatt ctcaagcctc ttagcggttc
     5761 aaaggtattt tctaaacccg tttccaggtg ttgtgaaagc caccgctaat tcaaagcaat
     5821 ccggagtata cggatccgcc accatgcccc cccagaggag caggaggagg aagagggcca
     5881 gcgccaccca gctgtaccag acctgcaagg ccagcggcac ctgccccccc gacgtgatcc
     5941 ccaaggtgga gggcaccacc ctggccgaca agatcctgca gtggggcagc ctgggcgtgt
     6001 tcttcggcgg cctgggcatc ggcaccggcg ccggcaccgg cggcaggacc ggctacgtgc
     6061 ccctgggcac caggcccccc gtgatcgccg agcccggccc cgccgtgagg ccccccatcg
     6121 ccgtggacac cgtgggcccc agcgacccca gcatcgtgag cctgctggag gagagcagcg
     6181 tgatcgacgc cggcatcacc gtgcccgaca tcaccagcca cggcggcttc aacatcacaa
     6241 ccagcaccgg cggccccgcc agcacccccg ccatcctgga catcagcccc cccaccaaca
     6301 ccatcagggt gaccaccacc accagcacca accccctgta catcgacccc ttcaccctgc
     6361 agccccccct gcccgccgag gtgaacggca ggctgctgat ctccaccccc accatcaccc
     6421 cccacagcta cgaggagatc cccatggaca ccttcgtggt gagcaccgac accaccaaca
     6481 ccttcaccag cacccccatc cccggcccca ggagcagcgc caggctgggc ctgtacagca
     6541 gggccaccca gcagaggccc gtgaccacca gcgccttcct gaccagcccc gccaggctgg
     6601 tgacctacga caaccccgcc tacgagggcc tgaccgagga caccctggtg ttcgagcacc
     6661 ccagcatcca caccgccccc gaccccgact tcatggacat cgtggccctg cacaggccca
     6721 tgctgagcag caagcagggc agcgtgaggg tgagcaggat cggccagagg ctgagcatgc
     6781 agaccaggag gggcaccagg ttcggcagca gggtgcactt cttccacgac ctgagcccca
     6841 tcacccacag cagcgagacc atcgagctgc agcccctgag cgccagcagc gtgagcgccg
     6901 ccagcaacat caacgacggc ctgttcgaca tctacgtgga caccagcgac gtgaacgtga
     6961 ccaacaccac cagcagcatc cccatgcacg gcttcgccac ccccaggctg agcaccacca
     7021 gcttccccac cctgcccagc atgagcaccc acagcgccaa caccaccatc cccttcagct
     7081 tccccgccac cgtgcacgtg ggccccgacc tgagcgtggt ggaccacccc tgggacagca
     7141 cccccaccag cgtgatgccc cagggcaact tcgtgatggt gagcggctgg gacttcatcc
     7201 tgcaccccag ctacttctgg aggaggagga ggaagcccgt gccctacttc ttcgccgacg
     7261 tgagggtggc cgcctaatag tctaggagca ggtttcccca atgacacaaa acgtgcaact
     7321 tgaaactccg cctggtcttt ccaggtctag aggggtaaca ctttgtactg cgtttggctc
     7381 cacgctcgat ccactggcga gtgttagtaa cagcactgtt gcttcgtagc ggagcatgac
     7441 ggccgtggga actcctcctt ggtaacaagg acccacgggg ccaaaagcca cgcccacacg
     7501 ggcccgtcat gtgtgcaacc ccagcacggc gactttactg cgaaacccac tttaaagtga
     7561 cattgaaact ggtacccaca cactggtgac aggctaagga tgcccttcag gtaccccgag
     7621 gtaacacgcg acactcggga tctgagaagg ggactggggc ttctataaaa gcgctcggtt
     7681 taaaaagctt ctatgcctga ataggtgacc ggaggtcggc acctttcctt tgcaattact
     7741 gaccctatga atacactgac tgtttgacaa ttaatcatcg gcatagtata tcggcatagt
     7801 ataatacgac tcactatagg agggccacca tgattgaaca agatggattg cacgcaggtt
     7861 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     7921 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     7981 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     8041 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     8101 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     8161 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     8221 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     8281 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     8341 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgaca catggcgatg
     8401 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     8461 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     8521 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     8581 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     8641 cgaaatgacc gaccaagcga attcgctagg attatcccta atacctgcca ccccactctt
     8701 aatcagtggt ggaagaacgg tctcagaact gtttgtttca attggccatt taagtttagt
     8761 agtaaaagac tggttaatga taacaatgca tcgtaaaacc ttcagaagga aaggagaatg
     8821 ttttgtggac cactttggtt ttcttttttg cgtgtggcag ttttaagtta ttagttttta
     8881 aaatcagtac tttttaatgg aaacaacttg accaaaaatt tgtcacagaa ttttgagacc
     8941 cattaaaaaa gttaaatgag aaacctgtgt gttcctttgg tcaacaccga gacatttagg
     9001 tgaaagacat ctaattctgg ttttacgaat ctggaaactt cttgaaaatg taattcttga
     9061 gttaacactt ctgggtggag aatagggttg ttttcccccc acataattgg aaggggaagg
     9121 aatatcattt aaagctatgg gagggttgct ttgattacaa cactggagag aaatgcagca
     9181 tgttgctgat tgcctgtcac taaaacaggc caaaaactga gtccttgggt tgcatagaaa
     9241 gctg
//
