LOCUS       sequence-81729-e        9205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV43 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV43 L1L2
FEATURES             Location/Qualifiers
     source          1..9205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     polyA_signal    3122..3243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3133..3152)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3187..3206
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3431..4019
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3920..3939
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4099..4402
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4517..5822
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4859..5790
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     regulatory      5841..5850
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7248..7692
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7725..7771
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7763..7781
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7791..8585
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7791..8585
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7845..7864)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8455..8474
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8632..9204
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg tggaggctga acgacaacaa ggtgtacctg
     1621 cccccccccg gccccatcgc cagcatcgtg agcaccgacg agtacgtgca gaggaccaac
     1681 ctgttctact acgccggcag cagcaggctg ctggccgtgg gccaccccta cttccccctg
     1741 aagaacagca gcggcaagat caccgtgccc aaggtgagcg gctaccagta cagggtgttc
     1801 agggtgaagc tgcccgaccc caacaagttc ggcttcagcg agaccaccct ggtcaccagc
     1861 gacacccaga ggctggtgtg gggctgcgtg ggcgtggaga tcggcagggg ccagcccctg
     1921 ggcgtgggca tcagcggcca cccctacctg aacaagtacg acgacaccga gaaccccagc
     1981 ggctacggca ccagccccgg ccaggacaac agggagaacg tggccatgga ctacaagcag
     2041 acccagctct gcatcgtggg ctgcaccccc cccatgggcg agtactgggg ccagggcgtg
     2101 ccctgcaacg ccagcggcgt gacccagggc gactgccccg tgatcgagct gaagagcgag
     2161 gtgatccagg acggcgacat ggtggacacc ggcttcggcg ccatggactt cgccagcctg
     2221 caggccagca agagcgacgt gcccctggac ctggtgaaca ccaagagcaa gtaccccgac
     2281 tacctgggca tggccgccga gccctacggc aacagcctgt tcttcttcct gaggagggag
     2341 cagatgttcc tgaggcactt cttcaacaag gccggcaaga ccggcgacgt ggtgcccagc
     2401 gacatgtaca tcgccggcag caacaccagg agcaagatcg ccgacagcat ctacttcagc
     2461 acccccagcg gcagcctggt gaccagcgac agccagctgt tcaacaagcc cctgtggatt
     2521 cagaaggccc agggccacaa caacggcatc tgcttcggca accagctgtt cgtgaccgtg
     2581 gtggacacca ccaggagcac caacctgacc ctgtgcgcca gcaccgaccc caccgtgccc
     2641 agcacctacg acaacgccaa gttcaaggag tacctgaggc acgtggagga gtacgacctg
     2701 cagttcatct tccagctgtg catcatcacc ctgaaccccg aggtgatgac ctacatccac
     2761 accatggacc ccaccctgct ggaggactgg aacttcggcg tgagcccccc cgccagcgcc
     2821 agcctggagg acacctacag gttcctgagc aacaaggcca tcgcctgcca gaagaacgcc
     2881 ccccccaagg agagggagga cccctacaag aagtacacct tctgggacat caacctgacc
     2941 gagaagttca gcgcccagct gacccagttc cccctgggca ggaagttcgt gatgcaggcc
     3001 ggcctgaggc ccaagcccaa gctgaagacc gtgaagagga gcgcccccag cagcagcacc
     3061 agcgcccccg ccagcaagag gaagaagacc aagaggtaat agtctagctg gccagacatg
     3121 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     3181 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     3241 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     3301 ttttaaagca agtaaaacct ctacaaatgt ggtatggaaa tgttaattaa ctagccatga
     3361 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3421 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3481 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3541 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3601 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3661 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3721 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3781 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3841 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3901 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3961 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4021 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4081 tcttaattaa cctgcaggcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     4141 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     4201 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     4261 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     4321 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     4381 attagtcatc gctattacca tgatgatgcg gttttggcag tacatcaatg ggcgtggata
     4441 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     4501 ttgactagtg gagccgagag taattcatac aaaaggaggg atcgccttcg caaggggaga
     4561 gcccagggac cgtccctaaa ttctcacaga cccaaatccc tgtagccgcc ccacgacagc
     4621 gcgaggagca tgcgcccagg gctgagcgcg ggtagatcag agcacacaag ctcacagtcc
     4681 ccggcggtgg ggggaggggc gcgctgagcg ggggccaggg agctggcgcg gggcaaactg
     4741 ggaaagtggt gtcgtgtgct ggctccgccc tcttcccgag ggtgggggag aacggtatat
     4801 aagtgcggta gtcgccttgg acgttctttt tcgcaacggg tttgccgtca gaacgcaggt
     4861 gagtggcggg tgtggcttcc gcgggccccg gagctggagc cctgctctga gcgggccggg
     4921 ctgatatgcg agtgtcgtcc gcagggttta gctgtgagca ttcccacttc gagtggcggg
     4981 cggtgcgggg gtgagagtgc gaggcctagc ggcaaccccg tagcctcgcc tcgtgtccgg
     5041 cttgaggcct agcgtggtgt ccgccgccgc gtgccactcc ggccgcacta tgcgtttttt
     5101 gtccttgctg ccctcgattg ccttccagca gcatgggcta acaaagggag ggtgtggggc
     5161 tcactcttaa ggagcccatg aagcttacgt tggataggaa tggaagggca ggaggggcga
     5221 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgt
     5281 ggctttccga agcaatcggg cgtgagttta gcctacctgg gccatgtggc cctagcactg
     5341 ggcacggtct ggcctggcgg tgccgcgttc ccttgcctcc caacaagggt gaggccgtcc
     5401 cgcccggcac cagttgcttg cgcggaaaga tggccgctcc cggggccctg ttgcaaggag
     5461 ctcaaaatgg aggacgcggc agcccggtgg agcgggcggg tgagtcaccc acacaaagga
     5521 agagggcctt gcccctcgcc ggccgctgct tcctgtgacc ccgtggtcta tcggccgcat
     5581 agtcacctcg ggcttctctt gagcaccgct cgtcgcggcg gggggagggg atctaatggc
     5641 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     5701 cattcttgga atttgcccct ttgagtttgg agcgaggcta attctcaagc ctcttagcgg
     5761 ttcaaaggta ttttctaaac ccgtttccag gtgttgtgaa agccaccgct aattcaaagc
     5821 aatccggagt atacggatcc gccaccatgg tgagccacac ccacaagcgg aggaagaggg
     5881 ccagcgccac ccagctgtac cagacctgca aggccgccgg cacctgcccc agcgacgtga
     5941 tcaacaaggt ggagcacacc accatcgccg accagatcct gaagtgggcc agcatgggcg
     6001 tgtacttcgg cggcctgggc atcggcaccg gcagcggcac cggcgggagg accggctacg
     6061 tgcccctgac caccggcagg accggcatcg tgcccaaggt gaccgccgag cccggcgtgg
     6121 tgagcaggcc ccccatcgtg gtggagagcg tggcccccac cgaccccagc atcgtgagcc
     6181 tgatcgagga gagcagcatc atccagagcg gcgcccccat caccaacatc cccagccacg
     6241 gcggcttcga ggtgaccagc agcggcagcg aggtgcccgc catcctggac gtcagcccca
     6301 gcaccagcgt gcacatcaca accagcaccc acctgaaccc cgccttcacc gaccccacca
     6361 tcgtgcagcc cacccccccc gtggaggccg gcggcaggat catcatcagc cacagcaccg
     6421 tgaccgccga cagcgccgag cagatcccca tggacacctt cgtgatccac agcgacccca
     6481 ccaccagcac ccccatcccc ggcaccgccc ccaggcccag gctgggcctg tacagcaagg
     6541 ccctgcagca ggtggagatc gtggacccca ccttcctgag cagcccccag aggctgatca
     6601 cctacgacaa ccccgtgttc gaggacccca acgccaccct gaccttcgag cagcccaccg
     6661 tgcacgaggc ccccgacagc aggttcatgg acatcgtgac cctgcacagg cccgccctga
     6721 ccagcaggag gggcatcgtg aggttcagca gggtgggcgc caggggcacc atgtacacca
     6781 ggagcggcat caggatcggc ggcagggtgc actttttcac cgacatcagc agcatcccca
     6841 ccgaggagag catcgagctg cagcccctgg gcaggagcca gagcttcccc accgtgagcg
     6901 acaccagcga cctgtacgat atctacgccg acgagaacct gctgaacaac gatatcagct
     6961 tcaccgacac ccacgtgagc ctgcagaaca gcaccaaggt ggtgaacacc gccgtgcccc
     7021 tggccaccgt gcccgacatc tacgcccaga ccggccccga catcagcttc cccaccatcc
     7081 ccatccacat cccctacatc cccgtgagcc ccagcatcag cccccagagc gtgagcatcc
     7141 acggcaccga cttctacctg caccccagcc tgtggcacct gggcaagagg aggaagaggt
     7201 tcagctactt cttcaccgac aactacgtgg ccgcctaata gtctaggagc aggtttcccc
     7261 aatgacacaa aacgtgcaac ttgaaactcc gcctggtctt tccaggtcta gaggggtaac
     7321 actttgtact gcgtttggct ccacgctcga tccactggcg agtgttagta acagcactgt
     7381 tgcttcgtag cggagcatga cggccgtggg aactcctcct tggtaacaag gacccacggg
     7441 gccaaaagcc acgcccacac gggcccgtca tgtgtgcaac cccagcacgg cgactttact
     7501 gcgaaaccca ctttaaagtg acattgaaac tggtacccac acactggtga caggctaagg
     7561 atgcccttca ggtaccccga ggtaacacgc gacactcggg atctgagaag gggactgggg
     7621 cttctataaa agcgctcggt ttaaaaagct tctatgcctg aataggtgac cggaggtcgg
     7681 cacctttcct ttgcaattac tgaccctatg aatacactga ctgtttgaca attaatcatc
     7741 ggcatagtat atcggcatag tataatacga ctcactatag gagggccacc atgattgaac
     7801 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     7861 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     7921 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     7981 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     8041 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     8101 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     8161 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     8221 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     8281 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     8341 tcgtcgtgac acatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     8401 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     8461 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     8521 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     8581 tctgagcggg actctggggt tcgaaatgac cgaccaagcg aattcgctag gattatccct
     8641 aatacctgcc accccactct taatcagtgg tggaagaacg gtctcagaac tgtttgtttc
     8701 aattggccat ttaagtttag tagtaaaaga ctggttaatg ataacaatgc atcgtaaaac
     8761 cttcagaagg aaaggagaat gttttgtgga ccactttggt tttctttttt gcgtgtggca
     8821 gttttaagtt attagttttt aaaatcagta ctttttaatg gaaacaactt gaccaaaaat
     8881 ttgtcacaga attttgagac ccattaaaaa agttaaatga gaaacctgtg tgttcctttg
     8941 gtcaacaccg agacatttag gtgaaagaca tctaattctg gttttacgaa tctggaaact
     9001 tcttgaaaat gtaattcttg agttaacact tctgggtgga gaatagggtt gttttccccc
     9061 cacataattg gaaggggaag gaatatcatt taaagctatg ggagggttgc tttgattaca
     9121 acactggaga gaaatgcagc atgttgctga ttgcctgtca ctaaaacagg ccaaaaactg
     9181 agtccttggg ttgcatagaa agctg
//
