LOCUS       sequence-81730-e        9187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV44 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV44 L1L2
FEATURES             Location/Qualifiers
     source          1..9187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     polyA_signal    3113..3234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3124..3143)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3178..3197
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3422..4010
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3911..3930
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4090..4393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4508..5813
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4850..5781
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     regulatory      5832..5841
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7230..7674
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7707..7753
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7745..7763
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7773..8567
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7773..8567
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7827..7846)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8437..8456
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8614..9186
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg tggaggccca gcgagaacca ggtgtacgtg
     1621 cccccccccg cccccgtgag caaggtgatc cccaccgacg cctacgtgaa gaggaccaac
     1681 atctactacc acgccagcag cagcaggctg ctggccgtgg gcaaccccta cttcgccatc
     1741 aggcccgcca acaagaccct ggtgcccaag gtgagcggct tccagtacag ggtgttcaag
     1801 atggtgctgc ccgaccccaa caagttcgcc ctgcccgaca ccagcatcta cgaccccacc
     1861 acccagaggc tggtgtgggc ctgcatcggc ctggaggtgg gcaggggcca gcccctgggc
     1921 gtgggcatca gcggccaccc cctgctgaac aagctggacg acgtggagaa cagcgccagc
     1981 tacgccgccg gccccggcca ggacaacagg gtgaacgtgg ccatggacta caagcagacc
     2041 cagctgtgcc tggtgggctg cgcccccccc ctgggcgagc actggggcaa gggcaagcag
     2101 tgcaacaacg tgagcgtgaa ggacggcgac tgcccccccc tggagctgat caccagcgtg
     2161 atcgaggacg gcgacatggt ggacaccggc ttcggcgcca tgaacttcgc cgagctgcag
     2221 cccaacaaga gcgacgtgcc cctggacatc tgcaccgcca cctgcaagta ccccgactac
     2281 ctgcagatgg ccgccgaccc ctacggcgac aggctgttct tctacctgag gaaggagcag
     2341 atgttcgcca ggcacttctt caacagggcc ggcaccgtgg gcgaggacgt gagccaggac
     2401 ctggtgatca agagcgccag caagaacacc gtgcccaacg ccatctactt caacaccccc
     2461 agcggcagcc tggtgagcag cgagacccag ctgttcaaca agcccttctg gctgcagaag
     2521 gcccagggcc acaacaacgg catctgctgg ggcaaccagc tgttcgtgac cgtggtggac
     2581 accaccagga gcaccaacat gaccatctgc gccgccacca cccagagccc ccccagcacc
     2641 tacaccagcg agcagtacaa gcagtacatg aggcacgtgg aggagttcga cctgcagttc
     2701 atgttccagc tgtgcagcat caccctgacc gccgaggtga tggcctacct gcacaccatg
     2761 aacgccggca tcctggagca gtggaacttc ggcctgagcc ccccccccaa cggcaccctg
     2821 gaggacaagt acaggtacgt gcagagccag gccatcacct gccagaagcc cccccccgag
     2881 aaggccaagc aggaccccta cgccaagctg agcttctggg aggtggacct gagggagaag
     2941 ttcagcagcg agctggacca gtaccccctg ggcaggaagt tcctgctgca gaccggcgtg
     3001 caggccagga gcagcgtgag ggtgggcagg aagaggcccg ccagcgccgc caccagcagc
     3061 agcaagcaga agaggagcag gaagaagtaa tagtctagct ggccagacat gataagatac
     3121 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     3181 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     3241 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     3301 aagtaaaacc tctacaaatg tggtatggaa atgttaatta actagccatg accaaaatcc
     3361 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3421 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3481 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3541 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3601 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3661 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3721 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3781 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3841 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3901 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3961 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4021 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttcttaatta
     4081 acctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     4141 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     4201 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     4261 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     4321 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     4381 cgctattacc atgatgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     4441 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttgactagt
     4501 ggagccgaga gtaattcata caaaaggagg gatcgccttc gcaaggggag agcccaggga
     4561 ccgtccctaa attctcacag acccaaatcc ctgtagccgc cccacgacag cgcgaggagc
     4621 atgcgcccag ggctgagcgc gggtagatca gagcacacaa gctcacagtc cccggcggtg
     4681 gggggagggg cgcgctgagc gggggccagg gagctggcgc ggggcaaact gggaaagtgg
     4741 tgtcgtgtgc tggctccgcc ctcttcccga gggtggggga gaacggtata taagtgcggt
     4801 agtcgccttg gacgttcttt ttcgcaacgg gtttgccgtc agaacgcagg tgagtggcgg
     4861 gtgtggcttc cgcgggcccc ggagctggag ccctgctctg agcgggccgg gctgatatgc
     4921 gagtgtcgtc cgcagggttt agctgtgagc attcccactt cgagtggcgg gcggtgcggg
     4981 ggtgagagtg cgaggcctag cggcaacccc gtagcctcgc ctcgtgtccg gcttgaggcc
     5041 tagcgtggtg tccgccgccg cgtgccactc cggccgcact atgcgttttt tgtccttgct
     5101 gccctcgatt gccttccagc agcatgggct aacaaaggga gggtgtgggg ctcactctta
     5161 aggagcccat gaagcttacg ttggatagga atggaagggc aggaggggcg actggggccc
     5221 gcccgccttc ggagcacatg tccgacgcca cctggatggg gcgaggcctg tggctttccg
     5281 aagcaatcgg gcgtgagttt agcctacctg ggccatgtgg ccctagcact gggcacggtc
     5341 tggcctggcg gtgccgcgtt cccttgcctc ccaacaaggg tgaggccgtc ccgcccggca
     5401 ccagttgctt gcgcggaaag atggccgctc ccggggccct gttgcaagga gctcaaaatg
     5461 gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg aagagggcct
     5521 tgcccctcgc cggccgctgc ttcctgtgac cccgtggtct atcggccgca tagtcacctc
     5581 gggcttctct tgagcaccgc tcgtcgcggc ggggggaggg gatctaatgg cgttggagtt
     5641 tgttcacatt tggtgggtgg agactagtca ggccagcctg gcgctggaag tcattcttgg
     5701 aatttgcccc tttgagtttg gagcgaggct aattctcaag cctcttagcg gttcaaaggt
     5761 attttctaaa cccgtttcca ggtgttgtga aagccaccgc taattcaaag caatccggag
     5821 tatacggatc cgccaccatg gcccacagca gggccaggcg gaggaagagg gccagcgcca
     5881 cccagctgta ccagacctgc aaggccgccg gcacctgccc cagcgacatc atccccaagg
     5941 tggagcacaa caccatcgcc gaccagatcc tgaagtgggg cagcctgggc gtgttcttcg
     6001 gcggcctggg catcggcacc ggcagcggca ccggcggcag gaccggctac atccccctgc
     6061 agagcacccc caggcccgac atccccagcg tgcccaccgc caggcccccc atcctggtgg
     6121 acaccgtggc ccccggcgac cccagcatcg tgagcctggt ggaggagagc gccatcatca
     6181 acagcggcgc ccccgagctg gtgcccccca gccacgccgg cttcgagatc accaccagcg
     6241 agagcaccac ccccgccatc ctggacgtga gcgtgaccac ccacaccacc agcaccagcg
     6301 tgttcaagaa ccccagcttc gccgacccca gcgtggtgca gagccagccc gccgtggagg
     6361 ccggcggcca catcctgatc agcaccagca gcatcagcag ccaccccgtg gaggagatcc
     6421 ccctggacac cttcatcgtg agcagcagcg acagcaaccc cgccagcagc acccccatcc
     6481 ccgccagcgg cgccaggccc aggatcggcc tgtacagcaa ggccctgcac caggtgcagg
     6541 tgaccgaccc cgccttcctg agcagccccc agaggctgat caccttcgac aaccccgcct
     6601 acgagggcga ggacgtgacc ctgcacttcg cccacaacac catccacgag ccccccgacg
     6661 acgccttcat ggacatcatc aggctgcaca ggcccgccat ccagagcagg aggggcaggg
     6721 tgaggttcag caggatcggc cagaggggca gcatgtacac caggagcggc aagcacatcg
     6781 gcggcaggat tcacttctac caggacatca gccccatcag cgccgccgcc gaggagatcg
     6841 agctgcaccc cctggtggcc accgcccagg acagcggcct gttcgacatc tacgccgagc
     6901 ccgaccccga cgtgaccgag gagcccgtga gcctgagctt cagcaccagc acccccttcc
     6961 agaggagcag cgtgagcgcc accccctggg gcaacaccac cgtgcccctg agcctgcccg
     7021 ccgacatgtt cgtgcagccc ggccccgaca tcatcttccc caccgccagc accaccaccc
     7081 cctacagccc cgtgaccccc gccctgccca ccggccccgt gttcatcagc ggcgccgcct
     7141 tctacctgta ccccacctgg tacttcgcca ggaagaggag gaagagggtg agcctgttct
     7201 tcgccgacgt ggccgcctaa tagtctagga gcaggtttcc ccaatgacac aaaacgtgca
     7261 acttgaaact ccgcctggtc tttccaggtc tagaggggta acactttgta ctgcgtttgg
     7321 ctccacgctc gatccactgg cgagtgttag taacagcact gttgcttcgt agcggagcat
     7381 gacggccgtg ggaactcctc cttggtaaca aggacccacg gggccaaaag ccacgcccac
     7441 acgggcccgt catgtgtgca accccagcac ggcgacttta ctgcgaaacc cactttaaag
     7501 tgacattgaa actggtaccc acacactggt gacaggctaa ggatgccctt caggtacccc
     7561 gaggtaacac gcgacactcg ggatctgaga aggggactgg ggcttctata aaagcgctcg
     7621 gtttaaaaag cttctatgcc tgaataggtg accggaggtc ggcacctttc ctttgcaatt
     7681 actgacccta tgaatacact gactgtttga caattaatca tcggcatagt atatcggcat
     7741 agtataatac gactcactat aggagggcca ccatgattga acaagatgga ttgcacgcag
     7801 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     7861 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     7921 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     7981 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     8041 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     8101 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     8161 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     8221 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     8281 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acacatggcg
     8341 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     8401 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     8461 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     8521 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     8581 gttcgaaatg accgaccaag cgaattcgct aggattatcc ctaatacctg ccaccccact
     8641 cttaatcagt ggtggaagaa cggtctcaga actgtttgtt tcaattggcc atttaagttt
     8701 agtagtaaaa gactggttaa tgataacaat gcatcgtaaa accttcagaa ggaaaggaga
     8761 atgttttgtg gaccactttg gttttctttt ttgcgtgtgg cagttttaag ttattagttt
     8821 ttaaaatcag tactttttaa tggaaacaac ttgaccaaaa atttgtcaca gaattttgag
     8881 acccattaaa aaagttaaat gagaaacctg tgtgttcctt tggtcaacac cgagacattt
     8941 aggtgaaaga catctaattc tggttttacg aatctggaaa cttcttgaaa atgtaattct
     9001 tgagttaaca cttctgggtg gagaataggg ttgttttccc cccacataat tggaagggga
     9061 aggaatatca tttaaagcta tgggagggtt gctttgatta caacactgga gagaaatgca
     9121 gcatgttgct gattgcctgt cactaaaaca ggccaaaaac tgagtccttg ggttgcatag
     9181 aaagctg
//
