LOCUS       sequence-81731-e        9403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV8 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV8 L1L2
FEATURES             Location/Qualifiers
     source          1..9403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     regulatory      1582..1591
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3155..3276
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3166..3185)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3220..3239
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3464..4052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3953..3972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4132..4435
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4550..5855
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4892..5823
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     regulatory      5874..5883
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7446..7890
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7923..7969
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7961..7979
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7989..8783
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7989..8783
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8043..8062)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8653..8672
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8830..9402
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg gccgtgtggc agagcgccac cggcaaggtg
     1621 tacctgcccc ccagcacccc cgtggccagg gtgcagagca ccgacgagta catccagagg
     1681 accaacatct actaccacgc caacaccgac aggctgctga ccgtgggcca cccttatttc
     1741 aacgtgtaca acaacaacgg cgacaccctg caggtgccca aggtgagcgg caaccagcac
     1801 agggtgttca ggctgaagct gcccgacccc aacaggttcg ccctggccga catgagcgtg
     1861 tacaaccccg acaaggagag gctggtgtgg gcctgcaggg gcctggagat cagcaggggc
     1921 cagcccctgg gcgtgggcag caccggccac ccctacttca acaaggtgaa ggacaccgag
     1981 aacagcaaca gctacaccac caccagcacc gacgacaggc agaacaccag cttcgacccc
     2041 aagcagatcc agatgttcat cgtgggctgc accccctgca tcggcgagca ctgggagaag
     2101 gccatcccct gcgccgagga ccagcagcag ggcctgtgcc cccccatcga gctcaagaac
     2161 accgtgatcg aggacggcga catggccgac atcggcttcg gcaacatgaa cttcaagacc
     2221 ctgcagcaga acaggagcga cgtgagcctg gacatcgtga acgagatctg caagtacccc
     2281 gacttcctga agatgcagaa cgacgtgtac ggcgacgcct gcttcttcta cgccaggagg
     2341 gagcagtgct acgccaggca cttcttcgtg aggggcggca agaccggcga cgacatcccc
     2401 gccgcccaga tcgacgacgg catgatgaag aaccagtact acatccccgg cggccaggac
     2461 cagagccaga aggacatcgg caacgccatg tacttcccca ccgtgagcgg cagcctggtg
     2521 agcagcgacg cccagctgtt caacaggccc ttctggctgc agagggccca gggccacaac
     2581 aacggcatcc tgtgggccaa ccagatgttc gtgaccgtgg tggacaacac caggaacacc
     2641 aacttcagca tcagcgtgta caccgagaac ggcgagctga agaacatcac cgactacaag
     2701 agcacccagt tcagggagta cctgaggcac gtggaggagt acgagatcag cctgatcctg
     2761 cagctgtgca agatccccct gaaggccgac gtgctggccc agatcaacgc catgaacagc
     2821 agcctgctgg aggagtggca gctgggcttc gtgcccaccc ccgacacccc catccacgac
     2881 acctacaggt acatcgacag cctggccacc aggtgccccg acaagagccc ccccaaggag
     2941 aagcccgacc cctacgccaa gttcaacttc tggaacgtgg acctgaccga gaggctgagc
     3001 ctggacctgg accagtacag cctgggcagg aagttcctgt tccaggccgg cctgcagcag
     3061 accaccgtga acggcaccaa gagcatcagc aggggcagcg tgaggggcac caagaggaag
     3121 aggaagaact aatagtctag ctggccagac atgataagat acattgatga gtttggacaa
     3181 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     3241 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     3301 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     3361 tgtggtatgg aaatgttaat taactagcca tgaccaaaat cccttaacgt gagttttcgt
     3421 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3481 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3541 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3601 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3661 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3721 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3781 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3841 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3901 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3961 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4021 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4081 tcctggcctt ttgctggcct tttgctcaca tgttcttaat taacctgcag gcgttacata
     4141 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     4201 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     4261 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     4321 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     4381 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatgatgat
     4441 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     4501 tctccacccc attgacgtca atgggagttt gttttgacta gtggagccga gagtaattca
     4561 tacaaaagga gggatcgcct tcgcaagggg agagcccagg gaccgtccct aaattctcac
     4621 agacccaaat ccctgtagcc gccccacgac agcgcgagga gcatgcgccc agggctgagc
     4681 gcgggtagat cagagcacac aagctcacag tccccggcgg tggggggagg ggcgcgctga
     4741 gcgggggcca gggagctggc gcggggcaaa ctgggaaagt ggtgtcgtgt gctggctccg
     4801 ccctcttccc gagggtgggg gagaacggta tataagtgcg gtagtcgcct tggacgttct
     4861 ttttcgcaac gggtttgccg tcagaacgca ggtgagtggc gggtgtggct tccgcgggcc
     4921 ccggagctgg agccctgctc tgagcgggcc gggctgatat gcgagtgtcg tccgcagggt
     4981 ttagctgtga gcattcccac ttcgagtggc gggcggtgcg ggggtgagag tgcgaggcct
     5041 agcggcaacc ccgtagcctc gcctcgtgtc cggcttgagg cctagcgtgg tgtccgccgc
     5101 cgcgtgccac tccggccgca ctatgcgttt tttgtccttg ctgccctcga ttgccttcca
     5161 gcagcatggg ctaacaaagg gagggtgtgg ggctcactct taaggagccc atgaagctta
     5221 cgttggatag gaatggaagg gcaggagggg cgactggggc ccgcccgcct tcggagcaca
     5281 tgtccgacgc cacctggatg gggcgaggcc tgtggctttc cgaagcaatc gggcgtgagt
     5341 ttagcctacc tgggccatgt ggccctagca ctgggcacgg tctggcctgg cggtgccgcg
     5401 ttcccttgcc tcccaacaag ggtgaggccg tcccgcccgg caccagttgc ttgcgcggaa
     5461 agatggccgc tcccggggcc ctgttgcaag gagctcaaaa tggaggacgc ggcagcccgg
     5521 tggagcgggc gggtgagtca cccacacaaa ggaagagggc cttgcccctc gccggccgct
     5581 gcttcctgtg accccgtggt ctatcggccg catagtcacc tcgggcttct cttgagcacc
     5641 gctcgtcgcg gcggggggag gggatctaat ggcgttggag tttgttcaca tttggtgggt
     5701 ggagactagt caggccagcc tggcgctgga agtcattctt ggaatttgcc cctttgagtt
     5761 tggagcgagg ctaattctca agcctcttag cggttcaaag gtattttcta aacccgtttc
     5821 caggtgttgt gaaagccacc gctaattcaa agcaatccgg agtatacgga tccgccacca
     5881 tggccagggc caggagggtg aagagggaca gcgtgaccca catctaccag acctgcaagc
     5941 aggccggcac ctgccccccc gacgtgatca acaaggtgga gcagaccacc gtggccgaca
     6001 acatcctgaa gtacggcagc gccggcgtgt tcttcggcgg cctgggcatc ggcaccggca
     6061 ggggcaccgg cggcgtgacc ggctacaccc ctctgagcga gggccccggc atcagggtgg
     6121 gcaacacccc caccgtggtg aggcccagcc tggtgcccga ggccgtgggc cccatggaca
     6181 tcctgcccat cgacaccatc gaccccgtgg agcccagcgt gagcagcgtg gtgcccctga
     6241 ccgagagcag cggcgccgac ctgctgcccg gcgaggtgga gaccatcgcc gagatccacc
     6301 ccgtgcccga gggccccacc atcgacagcc ccgtggtgac caccagcaag ggcagcagcg
     6361 ccatcctgga ggtggccccc gaacctaccc cccccaccag ggtgcgggtg agcaggaccc
     6421 agtaccacaa ccccagcttc cagatcatca ccgacagcac ccccacccag ggcgagagca
     6481 gcctggccga ccacatcctg gtgaccagcg gcagcggcgg ccagaccatc ggcagcgaca
     6541 tcaccgacgt gatcgagctg caggagttcc ccagcaggta cagcttcgag atcgacgagc
     6601 ccaccccccc caggcagagc agcaccccca tcgagaggcc ccaggtggtg ggcaggagga
     6661 ggggcatcag cctgaccaac aggaggctga tccagcaggt ggccgtggag gaccccctgt
     6721 tcctgagcaa gcccagcaag ctggtgaggt tcagcttcga caaccccgtg ttcgaggagg
     6781 aggtgaccaa catcttcgag caggacgtgg acatggtgga ggagcccccc gacagggact
     6841 tcctggacgt gaggcagctg ggcaggcccc agtacagcac cacccccgcc ggctacgtga
     6901 gggtgagcag gctgggcacc aggggcacca tcaggaccag gagcggcgcc cagatcggca
     6961 gccaggtgca cttctacagg gacctgagca gcatcaacac cgaggacccc atcgagctgc
     7021 agctgctggg ccagcacagc ggcgacagca ccatcgtgca gggccccgtg gagagcacct
     7081 tcgtgaacgt ggacatcagc gagaaccccc tgagcgagag catccaggcc ttcagcgacg
     7141 acctgctgct ggacgagacc gtggaggact tcagcggcag ccagctggtg atcggcaaca
     7201 ggaggagcac caccagctac accgtgccca ggttcgagac caccaggagc ggcagctact
     7261 acgtgcagga caccaagggc tactacgtgg cctaccccga gagcaggaac aacgaggaga
     7321 tcatctaccc cacccccgac ctgcccgtgg tgatcatcca cacccacgac aacagcggcg
     7381 acttcttcct gcaccccagc ctccggagga ggaagaggaa aaggaagtac ctgtaatagt
     7441 ctaggagcag gtttccccaa tgacacaaaa cgtgcaactt gaaactccgc ctggtctttc
     7501 caggtctaga ggggtaacac tttgtactgc gtttggctcc acgctcgatc cactggcgag
     7561 tgttagtaac agcactgttg cttcgtagcg gagcatgacg gccgtgggaa ctcctccttg
     7621 gtaacaagga cccacggggc caaaagccac gcccacacgg gcccgtcatg tgtgcaaccc
     7681 cagcacggcg actttactgc gaaacccact ttaaagtgac attgaaactg gtacccacac
     7741 actggtgaca ggctaaggat gcccttcagg taccccgagg taacacgcga cactcgggat
     7801 ctgagaaggg gactggggct tctataaaag cgctcggttt aaaaagcttc tatgcctgaa
     7861 taggtgaccg gaggtcggca cctttccttt gcaattactg accctatgaa tacactgact
     7921 gtttgacaat taatcatcgg catagtatat cggcatagta taatacgact cactatagga
     7981 gggccaccat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     8041 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     8101 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     8161 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     8221 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     8281 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     8341 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     8401 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     8461 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     8521 tgcccgacgg cgaggatctc gtcgtgacac atggcgatgc ctgcttgccg aatatcatgg
     8581 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     8641 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     8701 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     8761 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgaa
     8821 ttcgctagga ttatccctaa tacctgccac cccactctta atcagtggtg gaagaacggt
     8881 ctcagaactg tttgtttcaa ttggccattt aagtttagta gtaaaagact ggttaatgat
     8941 aacaatgcat cgtaaaacct tcagaaggaa aggagaatgt tttgtggacc actttggttt
     9001 tcttttttgc gtgtggcagt tttaagttat tagtttttaa aatcagtact ttttaatgga
     9061 aacaacttga ccaaaaattt gtcacagaat tttgagaccc attaaaaaag ttaaatgaga
     9121 aacctgtgtg ttcctttggt caacaccgag acatttaggt gaaagacatc taattctggt
     9181 tttacgaatc tggaaacttc ttgaaaatgt aattcttgag ttaacacttc tgggtggaga
     9241 atagggttgt tttcccccca cataattgga aggggaagga atatcattta aagctatggg
     9301 agggttgctt tgattacaac actggagaga aatgcagcat gttgctgatt gcctgtcact
     9361 aaaacaggcc aaaaactgag tccttgggtt gcatagaaag ctg
//
