LOCUS       sequence-81734-e        9232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV26 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV26 L1L2
FEATURES             Location/Qualifiers
     source          1..9232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     regulatory      1582..1591
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3122..3243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3133..3152)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3187..3206
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3431..4019
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3920..3939
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4099..4402
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4517..5822
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4859..5790
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     regulatory      5841..5850
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5845..5854
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7275..7719
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7752..7798
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7790..7808
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7818..8612
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7818..8612
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7872..7891)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8482..8501
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8659..9231
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg gccctgtgga ggaccagcga cagcaaggtg
     1621 tacctgcccc ccacccccgt gagcagggtg gtgaacaccg acgagtacgt gaccaggacc
     1681 ggcatctact actacgccgg cagcagcagg ctgctgaccc tgggccaccc ctacttcagc
     1741 atccccaaga ccggccagaa ggccgagatc cccaaggtga gcgcctacca gtacagggtg
     1801 ttcagggtgc acctgcctga ccccaacaag ttcggcctgc ccgaccccca gctgtacaac
     1861 cccgacaccg agaggctggt gtgggcctgc gtgggcgtgg aggtgggcag gggccagccc
     1921 ctgggcatcg gcctgagcgg ccaccccctg ttcaacaagc tggacgacac cgagaacagc
     1981 cacctggcca ccgtgaacgc cgacaccgac aacagggaca acgtgagcgt ggacaacaag
     2041 cagacccagc tgtgcatcat cggctgcacc ccccccctgg gcgagcactg gggcatcggc
     2101 accatctgca agaacaccca gacccagagg ggcgactgcc cccccctgga gctgatcagc
     2161 agcatcatcg aggacggcga catgatcgac accggcttcg gcgccatgga cttcaccgcc
     2221 ctgcaggcca ccaagagcga cgtgcccatc gacatcagcc agagcacctg caagtacccc
     2281 gactacctga agatgagcgc cgacacctac ggcaacagca tgttcttctt cctgaggagg
     2341 gagcagctgt tcgccaggca cttctacaac aaggccggcg ccgtgggcga cgccatcccc
     2401 accaccctgt acatcaaggg cgccgagagc ggcagggagc cccccaccag cagcatctac
     2461 agcgccaccc ccagcggcag catggtgacc agcgacgccc agctgttcaa caagccctac
     2521 tggctgcaga gggcccaggg ccacaacaac ggcatctgct ggggcaacca gctgttcgtg
     2581 acctgcgtgg acaccaccag gagcaccaac ctgaccatca gcaccctgag cgccgccagc
     2641 gccagcaccc ccttcaagcc cagcgactac aagcagttca tcaggcacgg cgaggagtac
     2701 gagctgcagt tcatcttcca gctgtgcaag atcaccctga ccaccgacgt gatggcctac
     2761 atccacctga tgaacgccag catcctggag gactggaact tcggcctgac cctgcccccc
     2821 accgccagcc tggaggacgc ctacaggttc atcaagaaca gcgccaccac ctgccagagg
     2881 aacgcccccc ccgtgcccaa ggaggacccc ttccagaagt tcaagttctg ggacgtggac
     2941 ctgaaggaga agttcagcat cgacctggac cagttccccc tgggcaggaa gttcatgctg
     3001 caggccggca tccagaggag gcccaagctg ggcaccaaga ggcccctgag cagcaccagc
     3061 agcagcacca agaggaagaa gaggaagctg accaagtaat agtctagctg gccagacatg
     3121 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     3181 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     3241 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     3301 ttttaaagca agtaaaacct ctacaaatgt ggtatggaaa tgttaattaa ctagccatga
     3361 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3421 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3481 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3541 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3601 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3661 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3721 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3781 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3841 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3901 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3961 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4021 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4081 tcttaattaa cctgcaggcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     4141 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     4201 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     4261 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     4321 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     4381 attagtcatc gctattacca tgatgatgcg gttttggcag tacatcaatg ggcgtggata
     4441 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     4501 ttgactagtg gagccgagag taattcatac aaaaggaggg atcgccttcg caaggggaga
     4561 gcccagggac cgtccctaaa ttctcacaga cccaaatccc tgtagccgcc ccacgacagc
     4621 gcgaggagca tgcgcccagg gctgagcgcg ggtagatcag agcacacaag ctcacagtcc
     4681 ccggcggtgg ggggaggggc gcgctgagcg ggggccaggg agctggcgcg gggcaaactg
     4741 ggaaagtggt gtcgtgtgct ggctccgccc tcttcccgag ggtgggggag aacggtatat
     4801 aagtgcggta gtcgccttgg acgttctttt tcgcaacggg tttgccgtca gaacgcaggt
     4861 gagtggcggg tgtggcttcc gcgggccccg gagctggagc cctgctctga gcgggccggg
     4921 ctgatatgcg agtgtcgtcc gcagggttta gctgtgagca ttcccacttc gagtggcggg
     4981 cggtgcgggg gtgagagtgc gaggcctagc ggcaaccccg tagcctcgcc tcgtgtccgg
     5041 cttgaggcct agcgtggtgt ccgccgccgc gtgccactcc ggccgcacta tgcgtttttt
     5101 gtccttgctg ccctcgattg ccttccagca gcatgggcta acaaagggag ggtgtggggc
     5161 tcactcttaa ggagcccatg aagcttacgt tggataggaa tggaagggca ggaggggcga
     5221 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgt
     5281 ggctttccga agcaatcggg cgtgagttta gcctacctgg gccatgtggc cctagcactg
     5341 ggcacggtct ggcctggcgg tgccgcgttc ccttgcctcc caacaagggt gaggccgtcc
     5401 cgcccggcac cagttgcttg cgcggaaaga tggccgctcc cggggccctg ttgcaaggag
     5461 ctcaaaatgg aggacgcggc agcccggtgg agcgggcggg tgagtcaccc acacaaagga
     5521 agagggcctt gcccctcgcc ggccgctgct tcctgtgacc ccgtggtcta tcggccgcat
     5581 agtcacctcg ggcttctctt gagcaccgct cgtcgcggcg gggggagggg atctaatggc
     5641 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     5701 cattcttgga atttgcccct ttgagtttgg agcgaggcta attctcaagc ctcttagcgg
     5761 ttcaaaggta ttttctaaac ccgtttccag gtgttgtgaa agccaccgct aattcaaagc
     5821 aatccggagt atacggatcc gccaccatgg tggccgtgag ggcccccagg aggaagaggg
     5881 ccagcgccac cgacctgtac aagacctgca aggccgccgg cacctgcccc cccgacgtga
     5941 tccccaagat cgagggcagc accctggccg acaagatcct gcagtggagc gggctgggca
     6001 tcttcctggg cggcctgggc atcggcaccg gcaccggcag cggcggcagg accggctaca
     6061 tccccctggg cggcggcggc aggcccagcg tggtggacat cggccccacc aggcccccca
     6121 tcatcatcga gcccgtgggc cccaccgagc ccagcatcgt gaccctggtg gaggagagca
     6181 gcatcatcca gagcggcgcc cccatcccca ccttcagcgg cggcaacggc ttcgagctga
     6241 ccaccagcag cgccaccacc cccgccgtgc tggacatcac ccccagcgcc ggcaccgtgc
     6301 acgtgaccag caccaacatc cagaaccccc tgtacatcga gccccccatc gacatccccc
     6361 aggccggcga ggccagcggc cacatcttca ccaccaccag caccgccggc acccacagct
     6421 acgaggagat ccccatggag gtgttcgcca gcaccaacgg caccggcctg gagcccatca
     6481 gcagcacccc catccccggc atccagaggg tgagcgcccc caggctgtac agcaaggcct
     6541 accagcaggt gaaggtgacc gaccccaact tcatcggcaa ccccagcacc ttcgtgacct
     6601 tcgacaaccc cgcctacgag cccatcgacg agaccctgac ctacgccagc agcagcaccg
     6661 tggcccccga ccccgacttc ctggacatca tcgccctgca caggcccgcc ctgaccagca
     6721 ggaagggcac cgtgaggtac agcaggctgg gccagaaggc caccatgaag accaggagcg
     6781 gcaagcagat cggcgccacc gtgcactact accacgacat cagccccatc cagagcttcg
     6841 ccgagcacga ggagatcgag ctgcagcccc tgcacaccag cacccacagc agcgcccccc
     6901 tgttcgacat ctacgccgac cccgacaccg tgcccagcat ccacaccccc aggatgagct
     6961 acagccccac caccctgccc gtgcccaggt acgccagcaa cgtgttcagc agcatcaaca
     7021 ccagcaccac caacgtgacc gtgcccctga gcaccagctt cgagctgccc gtgtacagcg
     7081 gcagcgacat ctacaccccc accagcagcc ccacctggcc tagcctgccc ccccccccca
     7141 ccaccaacct gcccgccatc gtggtgcacg gcgacaacta ctacctgtgg ccctacatct
     7201 acctgatcca caagaggagg aagaggatgc cctacttctt cagcgacggc ttcgtggcct
     7261 actaatagtc taggagcagg tttccccaat gacacaaaac gtgcaacttg aaactccgcc
     7321 tggtctttcc aggtctagag gggtaacact ttgtactgcg tttggctcca cgctcgatcc
     7381 actggcgagt gttagtaaca gcactgttgc ttcgtagcgg agcatgacgg ccgtgggaac
     7441 tcctccttgg taacaaggac ccacggggcc aaaagccacg cccacacggg cccgtcatgt
     7501 gtgcaacccc agcacggcga ctttactgcg aaacccactt taaagtgaca ttgaaactgg
     7561 tacccacaca ctggtgacag gctaaggatg cccttcaggt accccgaggt aacacgcgac
     7621 actcgggatc tgagaagggg actggggctt ctataaaagc gctcggttta aaaagcttct
     7681 atgcctgaat aggtgaccgg aggtcggcac ctttcctttg caattactga ccctatgaat
     7741 acactgactg tttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgactc
     7801 actataggag ggccaccatg attgaacaag atggattgca cgcaggttct ccggccgctt
     7861 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     7921 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     7981 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     8041 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     8101 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     8161 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     8221 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     8281 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     8341 aggcgagcat gcccgacggc gaggatctcg tcgtgacaca tggcgatgcc tgcttgccga
     8401 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     8461 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     8521 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     8581 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     8641 ccaagcgaat tcgctaggat tatccctaat acctgccacc ccactcttaa tcagtggtgg
     8701 aagaacggtc tcagaactgt ttgtttcaat tggccattta agtttagtag taaaagactg
     8761 gttaatgata acaatgcatc gtaaaacctt cagaaggaaa ggagaatgtt ttgtggacca
     8821 ctttggtttt cttttttgcg tgtggcagtt ttaagttatt agtttttaaa atcagtactt
     8881 tttaatggaa acaacttgac caaaaatttg tcacagaatt ttgagaccca ttaaaaaagt
     8941 taaatgagaa acctgtgtgt tcctttggtc aacaccgaga catttaggtg aaagacatct
     9001 aattctggtt ttacgaatct ggaaacttct tgaaaatgta attcttgagt taacacttct
     9061 gggtggagaa tagggttgtt ttccccccac ataattggaa ggggaaggaa tatcatttaa
     9121 agctatggga gggttgcttt gattacaaca ctggagagaa atgcagcatg ttgctgattg
     9181 cctgtcacta aaacaggcca aaaactgagt ccttgggttg catagaaagc tg
//
