LOCUS       sequence-8176-ex        5174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Li X, Massey HC, Nolan TJ, Schad GA, Kraus K, Sundaram M, Lok JB
  TITLE     Successful transgenesis of the parasitic nematode Strongyloides 
            stercoralis requires endogenous non-coding control elements.
  JOURNAL   Int J Parasitol. 2006 May 31. 36(6):671-9.
  PUBMED    16500658
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J 
            Parasitol. 2006 May 31. 36(6):671-9."
COMMENT     Sequence Label: pPV230.13
FEATURES             Location/Qualifiers
     source          1..5174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3..19
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3..19
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1262..1290)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2038..2058
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(2764..2786)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2886..2905
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2983..3001)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3069..3173
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3174..3242,3243..4034)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3392..3411)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4205..4793
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4694..4713
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4947..4964
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5081..5102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5117..5122,5123..5140,5141..5147)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5155..5171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cacaggaaac agctatgacc atgattacgc caagctgtaa gtttaaacat gatcttacta
       61 actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac tcacaacgat
      121 ggatacgcta acaacttgga aatgaaataa gcttacgagc agtacagata cccttggcct
      181 ttcccattat ttaacctaaa aaataatttt aaatattata gtttaataac aaaatcaaaa
      241 tatcgataga taaaaaaatt acaaactaca atttaaatta gctagaaaaa ttctaaatga
      301 attgaaactt aattttaaaa attttttaat acatatatta aatataataa ggtacaatta
      361 aggctaaaaa atttaaaaaa ttaaaaaatg cgtattaata aaatgtacaa ttttcaaatt
      421 gtaaatgctg gtcaagcgtt tcaagaggca gcctaataaa caatggtggg ataatgtaat
      481 cgtcatcaaa aatgatgatg tagccataga ggagagttgc atttatttta ctttgtttaa
      541 aggttaatat tcttacttat caatatattt gttttatgtt tgttacttaa aatattttga
      601 aatcctgctt tatatacctc ctttatgcta aattttagtt aaaatagata acaaataaca
      661 ttgttttttt atctcgtaat tttcataata atttgattca atttaacatt tttcagatta
      721 ctttgaattg ttttggcttt ttttaaacta aaatttctaa attaactatt caacatggct
      781 accgttccaa cgtaagttta acaatttcat tgattcaatt aacatatttt ccagtgagaa
      841 gtcggctgat gaaaagaaga atgataaact tgctacagct attttacaaa ccaaagctaa
      901 accaaatcgt ttaatggtag accaatcaaa tgatgatgat aattctacaa ttttcttaaa
      961 ccaaactaaa gttgacgagc ttggactctt ccgtggtgat actgttctta ttaaaggaaa
     1021 gaaaagacgt gaaacggttg ctattgttgt tgttgacgat gaatgccctg tggataagat
     1081 aaaaatgaac agggttgttc gtaataacct tcctgcaggt cgactagagg atccccggga
     1141 ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca
     1201 ggaggaccct tggagggtac cggtagaaaa aatgagtaaa ggagaagaac ttttcactgg
     1261 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
     1321 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
     1381 tggaaaacta cctgttccat gggtaagttt aaacatatat atactaacta accctgatta
     1441 tttaaatttt cagccaacac ttgtcactac tttctgttat ggtgttcaat gcttctcgag
     1501 atacccagat catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt
     1561 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgta agtttaaaca
     1621 gttcggtact aactaaccat acatatttaa attttcaggt gctgaagtca agtttgaagg
     1681 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     1741 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     1801 acaaaagaat ggaatcaaag ttgtaagttt aaacatgatt ttactaacta actaatctga
     1861 tttaaatttt cagaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
     1921 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     1981 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     2041 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
     2101 gcattcgtag aattcctagg aatttatttt gataagtctc gcgtatttta tttgtaataa
     2161 tnttataata agatcaattt acatttatac tgataaaatt gacacaatta cactttttat
     2221 tataaaatat tttaaaatat ttctatcaaa aggtttattt ttgttttaaa tataatggtt
     2281 ttttttgtaa aaaatactta tttcgtttga taatctttat tcagagattt gaataatttt
     2341 aaataaagta gtttagtttt catataaaag ttaatggtat atataaatat ttaaatagta
     2401 aagattgaat cagtattcaa aatgacacct aaaagttatt ctatttctag ctataacaat
     2461 aataataaag ataattatga tacagattta gaaaaaaaaa taaggttaaa ttaattaatt
     2521 ctatttttaa ttaaaatgat ttaagggagt taaatgaaac agaaacagat tgggtctcta
     2581 tatatttagt ttatgtaatg ttattttttg gtggaataca aataacggta tattttactt
     2641 caatgtggcc atacttatca acaataaata gtaaggcaaa tgtagatact ttagggtggg
     2701 ttgtagcact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga
     2761 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     2821 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     2881 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     2941 cagatgcgta aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat
     3001 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     3061 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3121 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3181 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3241 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3301 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3361 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     3421 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     3481 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3541 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3601 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3661 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3721 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3781 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3841 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     3901 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     3961 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4021 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4081 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4141 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4201 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4261 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4321 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     4381 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4441 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4501 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4561 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     4621 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4681 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4741 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4801 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4861 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4921 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4981 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     5041 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     5101 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     5161 gcggataaca attt
//
