LOCUS       sequence-81766-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  JxOFF-pre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Kim J, Zhao T, Petralia RS, Yu Y, Peng H, Myers E, Magee JC
  TITLE     mGRASP enables mapping mammalian synaptic connectivity with light 
            microscopy.
  JOURNAL   Nat Methods. 2011 Dec 4;9(1):96-102. doi: 10.1038/nmeth.1784.
  PUBMED    22138823
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-4- 4"; volume: "9"; 
            issue: "1"; pages: "96-102"
COMMENT     Sequence Label: paavCAG-JxOFF-pre-mGRASP-mCerulean
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(29..62)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aatccagagg ttgattatcg ataagctgta ccgttcgtat agcatacatt atacgaagtt
       61 atagatctga tagcttgatg tcaaactctc acttgtacag ctcatccata cccagggtta
      121 tgccggcagc tgtgacaaac tccagcagta ccatatgatc tctcttttcg ttggggtcct
      181 tgctcagctt gctctgtgta ctaaggtagt gattgtcggg caacagcaca ggtccatcac
      241 cgatgggagt attctgttgg taatggtcag ccagctgaac gcttccatcc tctatgttgt
      301 gccggatttt gaaattggcc tttatgccat ttttctgctt gtcggcggtt atgtaaacat
      361 tatcagatat ggcgttgtat tccagcttgt ggccgagaat gttcccatct tctttgaagt
      421 cgattccctt gagttcaatc ctgttcacca aggtgtcacc ttcgaatttc acctccgccc
      481 ttgtcttgta gttaccatcg tccttgaaaa atatcgtcct ttcctgaaca tacccctcgg
      541 gcatagcgga cttgaagaag tcgtgctgct tcatgtggtc agggtaccgg gcgaagcact
      601 gtacacccca tgtgagggtg gtgacgagtg tgggccaagg cacggggagc ttccctgttg
      661 tacagatgaa cttcagcgtc agtttgccgt aggtggcatc cccttctccc tcgccgctta
      721 cggagaactt gtgcccattt acatctccgt cgagctccac caggataggc acgacccctg
      781 taaacaactc ttcgccct
//
