LOCUS       sequence-8177-ex        4226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Junio AB, Li X, Massey HC, Nolan TJ, Todd Lamitina S, Sundaram MV, 
            Lok JB
  TITLE     Strongyloides stercoralis: cell- and tissue-specific transgene 
            expression and co-transformation with vector constructs 
            incorporating a common multifunctional 3' UTR.
  JOURNAL   Exp Parasitol. 2008 Feb . 118(2):253-65.
  PUBMED    17945217
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Parasitol. 2008 Feb . 118(2):253-65."
COMMENT     Sequence Label: pAJ01
FEATURES             Location/Qualifiers
     source          1..4226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(164..192)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     940..960
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(1666..1688)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1788..1807
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1885..1903)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1971..2075
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2076..2144,2145..2936)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2294..2313)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3107..3695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3596..3615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3849..3866
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3983..4004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4019..4024,4025..4042,4043..4049)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4057..4073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4062..4084
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4081..4097
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4081..4097
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa aggacccaaa
       61 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttggagggt
      121 accggtagaa aaaatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt
      181 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      241 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      301 atgggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt ttcagccaac
      361 acttgtcact actttctgtt atggtgttca atgcttctcg agatacccag atcatatgaa
      421 acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
      481 tttcaaagat gacgggaact acaagacacg taagtttaaa cagttcggta ctaactaacc
      541 atacatattt aaattttcag gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
      601 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
      661 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
      721 agttgtaagt ttaaacatga ttttactaac taactaatct gatttaaatt ttcagaactt
      781 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      841 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      901 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      961 agctgctggg attacacatg gcatggatga actatacaaa tagcattcgt agaattccta
     1021 ggaatttatt ttgataagtc tcgcgtattt tatttgtaat aatnttataa taagatcaat
     1081 ttacatttat actgataaaa ttgacacaat tacacttttt attataaaat attttaaaat
     1141 atttctatca aaaggtttat ttttgtttta aatataatgg ttttttttgt aaaaaatact
     1201 tatttcgttt gataatcttt attcagagat ttgaataatt ttaaataaag tagtttagtt
     1261 ttcatataaa agttaatggt atatataaat atttaaatag taaagattga atcagtattc
     1321 aaaatgacac ctaaaagtta ttctatttct agctataaca ataataataa agataattat
     1381 gatacagatt tagaaaaaaa aataaggtta aattaattaa ttctattttt aattaaaatg
     1441 atttaaggga gttaaatgaa acagaaacag attgggtctc tatatattta gtttatgtaa
     1501 tgttattttt tggtggaata caaataacgg tatattttac ttcaatgtgg ccatacttat
     1561 caacaataaa tagtaaggca aatgtagata ctttagggtg ggttgtagca ctagtcggcc
     1621 gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     1681 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     1741 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     1801 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     1861 ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag gttaatgtca
     1921 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1981 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2041 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     2101 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2161 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2221 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2281 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     2341 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2401 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2461 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2521 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2581 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     2641 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2701 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2761 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     2821 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2881 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2941 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3001 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3061 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3121 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3181 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3241 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3301 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3361 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3421 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3481 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3541 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3601 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3661 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3721 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3781 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3841 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3901 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3961 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4021 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4081 caggaaacag ctatgaccat gattacgcca agctgtaagt ttaaacatga tcttactaac
     4141 taactattct catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg
     4201 atacgctaac aacttggaaa tgaaat
//
