LOCUS       sequence-81771-e        8224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses C. elegans DRE-1 in mammalian cells with RGS-6xHis tag at 
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M,
            Antebi A
  TITLE     DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. 
            elegans Developmental Timing and Maturation.
  JOURNAL   Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028.
  PUBMED    24613396
REFERENCE   2  (bases 1 to 8224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 
            10.1016/j.devcel.2014.01.028."
COMMENT     Sequence Label: RGS-6xHis-DRE-1-pcDNA3.1-
FEATURES             Location/Qualifiers
     source          1..8224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             917..934
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3818..3835)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3824..4048
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4094..4522
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4181..4200)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4391..4412
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4531..4551)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4536..4865
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4716..4851
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4778..4797
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4932..5726
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4986..5005)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5596..5615
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5900..6021
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5937..5956)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5991..6010
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6070..6086)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6070..6086)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6083..6105)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6094..6110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6118..6124,6125..6142,6143..6148))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6163..6184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6301..6318)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6472..7057)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6552..6571)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7228..8019,8020..8088))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7851..7870
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8089..8193)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatgaga ggatcgcatc accatcacca tcacgggccc tctagactcg agcggccgct
      961 tatgtcgtcc tcttcgtcac cattcttcca tcctccacct caacaagtga tggatgatac
     1021 gacacagcaa tcgcccagtt actcacaaaa ctcgaattct ccatcacaac atacgttcga
     1081 gtcaatggaa cctgctgctg gaggatcaac gtcgatgaga tattcaccat cgggaagctc
     1141 cgaagcagac aatccgacac tcggattgtt ttctgcacaa gcctcttatc catactccct
     1201 ccgaaaacgt cggccttgtt tgacaaagga tgaggatttg tgcttcccga ttacagctgc
     1261 aacagctgat gagacacctc aacccacgat gaaaaagttc aagctggaat gtctttcacc
     1321 tggcgaagct cgagatttct cgattgaaga cgagagcggc acaaacctgg aagccaaaat
     1381 ggagacaacg gaatgtgaac cagaagaaga ggaggcggtg gaagaagaag atcaacaaga
     1441 tcatatcaat agactccccg aagaacttct tctcaaagtg ttttcatttc ttcctgataa
     1501 atcgctgctt gcatgtagtt cagtctcata ccgattcaat caaatttcga atagtcatga
     1561 agtgtggaag gagctgtatt gtaatttata tgactatcga attccacttt tccacccatc
     1621 tcatgcaaaa ttcgagttcc gggaacagag ccgttggcgg gatggtaatc catggaaaga
     1681 gtcccatcga cagcttcatc atggagttca tgtgatgaaa gagccacggg tcaatcttcg
     1741 atctgtcaac tatcgttgtt tcgatcaaat tgagaaggct caatcgttct tggaggagga
     1801 tgagtataga gagaaactta ttttcttgca cactggtgtt catgagccaa tcgacacaat
     1861 tctcatcaac acagatgtgc agattatcgg agcaagtgac tcacgagata tcacatcttc
     1921 ggttgttctc gaaggaagta aaaataccgc attgacattc actgatggaa gtgcaaacgc
     1981 gtatttcgga ttcatcacag tccggtttcg ggctgatcca gtgtgcaggc agcagccaca
     2041 gattgctcag caggctcaac aaatgaatca tttctattcg attttagtga ctgataagga
     2101 tgcaatgcca tatattgaaa gatgtgatat cacttcgaaa gttggaaatg gtgcagctgt
     2161 atgtgtgaag aaaagtgcgg ctcctaaatt caaatattgc acagttcttg attgtgaaaa
     2221 tgttggaatc tacattactg acaacgccac cggacactat gagcactgtg aaattgctcg
     2281 aaatactttg gctggtgtct gggtgaaaaa tcatgcgaat ccctacttcc gaaagtgtac
     2341 aattcattct ggaaaggatg tgggagtttt cacatttgaa catggtcagg gttactttga
     2401 aaagtgcaat atccattcaa atcggatatc tggcatcgag gtgaaaaatt cggcaaatcc
     2461 ggttgtgatt cgatgcgaag tgcatcatgg ttacactgga ggtatctatg ttcatgagag
     2521 aggacgtggc cagtttatgg agaatcggat atatgcgaac gcgtatgcag gtatctggat
     2581 cacttcgcat tctgatccga ctatccgaaa gaatgagatt ttcactggac aacagggtgg
     2641 agtctacatc tttggtgaag gacgaggact catcgagcag aataatattt atggaaacgc
     2701 gttggctgga attcagatcc gctcacaatc cgatccaatc gtaagattga ataagattca
     2761 cgatggattg cacggaggaa tttatgttca cgagaaagga agaggattga ttgaagagaa
     2821 tgaagtttat ggaaatacgt tggcaggaat ctgggtaaca actggttcaa gtccaattct
     2881 gcgcaaaaat agaattcatt ctggaaaaca ggtcggagta tacttctacg atcaaggtca
     2941 cggacttctt gaagaaaatg atattttcaa tcatttgtat tccggagtgc aaattcgaac
     3001 gggatcaaac ccaaaaatca caaggaataa gatttgggga ggtcagaatg gaggtgtact
     3061 cgtgtacaat ggaggaaagg gatgcttgga agacaatgaa atcttcgata atgcaatggc
     3121 gggtgtctgg atcaaaacag acagtgagcc aactcttcga cgaaacaaga tctacgacgg
     3181 aagagatggc ggagtttgta ttttcaatcg tggtaaaggt cttctcgagg acaatgaaat
     3241 cttccgaaat gcgcaagctg gtgttctcat ttcaaccgaa tcgaatccaa ctctacgaag
     3301 aaatcgagta tttgatggaa aatcggcggg aatcgagatc acgaatggag cgacagctac
     3361 actagaagaa aatcaattgt tcagaaataa atatggagga ctttgcgtag caaccggtgt
     3421 cactccagtg caacgtggaa atcgtatcta tgacaaccat gacacaattt ctagagctat
     3481 caagactggc ctgtgtctct tcaaagtgag cagcaacaac agttttccga tgcacaattt
     3541 ctatagatgt accacgtgta acacgacgga acgtaatgcg atctgcacca actgcatcag
     3601 aacatgccat cgtggtcatt ctgtagaact tgtgaggttc gatagattct tctgcgactg
     3661 tggtgctgga actctcgaac ggcactgtca cctgcaaaat gtgccacgtg acaacgacac
     3721 tgtctatgac tcagcgacgc cgatctccac tgagactggc accgaaattt aaggatccga
     3781 gctcggtacc aagcttaagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     3841 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     3901 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     3961 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     4021 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     4081 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4141 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4201 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4261 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4321 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4381 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4441 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4501 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     4561 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4621 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4681 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4741 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4801 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     4861 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     4921 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     4981 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     5041 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     5101 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     5161 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     5221 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     5281 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     5341 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     5401 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     5461 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     5521 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     5581 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     5641 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     5701 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     5761 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     5821 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     5881 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     5941 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6001 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     6061 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6121 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6181 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6241 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6301 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6361 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6421 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6481 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6541 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6601 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6661 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6721 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6781 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6841 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6901 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6961 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg
     7021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8161 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8221 cgtc
//
