LOCUS       sequence-81775-e        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CMV driven mammalian expression of UBR5 with an N-termianl GFP 
            fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Gudjonsson T, Altmeyer M, Savic V, Toledo L, Dinant C, Grofte M, 
            Bartkova J, Poulsen M, Oka Y, Bekker-Jensen S, Mailand N, Neumann B,
            Heriche JK, Shearer R, Saunders D, Bartek J, Lukas J, Lukas C
  TITLE     TRIP12 and UBR5 suppress spreading of chromatin ubiquitylation at 
            damaged chromosomes.
  JOURNAL   Cell. 2012 Aug 17;150(4):697-709. doi: 10.1016/j.cell.2012.06.039. 
            Epub 2012 Aug 9.
  PUBMED    22884692
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.06.039"; journalName: "Cell"; date: 
            "2012-08-17- 17"; volume: "150"; issue: "4"; pages: "697-709"
COMMENT     Sequence Label: GFP-UBR5
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    255..303
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     328..347
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(387..428)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    436..460
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtggggata
       61 ccccctagag ccccagctgc gcagatctgc tatggcaggg cctgccgccc cgacgttggc
      121 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg
      181 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg
      241 aaccccgcgt ttatgaacaa acgacccaac acccgtgcgt tttattctgt ctttttattg
      301 ccgtcatagc gcgggttcct tccggtattg tctccttccg tgtttcagtt agcctccccc
      361 gtttaaactc attactaacc ggtacgcgta gaatcgagac cgaggagagg gttagggata
      421 ggcttcccgt tatcaaccac tttgtacaag aaagctgggt ccgcggctac acaaaaccaa
      481 aattcttggt cttaatggcg agtaacaatt tctgtttgag aatctgttta gaggaataga
      541 gtgggacgta aagtcgagaa atgcaagtat ttgcagtagg aagatgttgg tcatctggtg
      601 gtcttattgt gattgagggc ataggctgga atccttcttc actggctggc agtgatgggc
      661 ttgatgtcca aaagtaaaca agatcttgtc gttctgtcat gctcatcttc tctactattg
      721 accagaacca acgcttgaac tgcagaagct tctcagcatt ttctcctgat tcatcattga
      781 aagaggtaaa actgatcagc atttgcacat tgacttcacc gcagccattt accaaaagcc
      841 taaaatcttc tgccgttaaa tcttctaatg aattttttgg aagcacatct agtagacctt
      901 tcctcattgc atgtaagggc tgttctgcaa ctaccaacat tctgtgttct gcgtatttcc
      961 gcacatactc atatacattc tgtggagtga ctggtatatt tacaccatta ggaatgagtt
     1021 caacctgtcc tccaccttct tcttta
//
