LOCUS       sequence-81777-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CMV driven mammalian expression of ligase-dead UBR5 with an 
            N-termianl GFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Gudjonsson T, Altmeyer M, Savic V, Toledo L, Dinant C, Grofte M, 
            Bartkova J, Poulsen M, Oka Y, Bekker-Jensen S, Mailand N, Neumann B,
            Heriche JK, Shearer R, Saunders D, Bartek J, Lukas J, Lukas C
  TITLE     TRIP12 and UBR5 suppress spreading of chromatin ubiquitylation at 
            damaged chromosomes.
  JOURNAL   Cell. 2012 Aug 17;150(4):697-709. doi: 10.1016/j.cell.2012.06.039. 
            Epub 2012 Aug 9.
  PUBMED    22884692
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.06.039"; journalName: "Cell"; date: 
            "2012-08-17- 17"; volume: "150"; issue: "4"; pages: "697-709"
COMMENT     Sequence Label: GFP-UBR5 ∆HECT
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    257..305
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     330..349
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(389..430)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    438..462
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtgggga
       61 taccccctag agccccagct gcgcagatct gctatggcag ggcctgccgc cccgacgttg
      121 gctgcgagcc ctgggccttc acccgaactt ggggggtggg gtggggaaaa ggaagaaacg
      181 cgggcgtatt ggccccaatg gggtctcggt ggggtatcga cagagtgcca gccctgggac
      241 cgaaccccgc gtttatgaac aaacgaccca acacccgtgc gttttattct gtctttttat
      301 tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc
      361 ccgtttaaac tcattactaa ccggtacgcg tagaatcgag accgaggaga gggttaggga
      421 taggcttccc gttatcaacc actttgtaca agaaagctgg gtccgcggct acacaaaacc
      481 aaaattcttg gtcttaatgg cgagtaacaa tttctgtttg agaatctgtt tagaggaata
      541 gagtgggacg taaagtcgag aaatggcagt atttgcagta ggaagatgtt ggtcatctgg
      601 tggtcttatt gtgattgagg gcataggctg gaatccttct tcactggctg gcagtgatgg
      661 gcttgatgtc caaaagtaaa caagatcttg tcgttctgtc atgctcatct tctctactat
      721 tgaccagaac caacgcttga actgcagaag cttctcagca ttttctcctg attcatcatt
      781 gaaagaggta aaactgatca gcatttgcac attgacttca ccgcagccat ttaccaaaag
      841 cctaaaatct tctgccgtta aatcttctaa tgaatttttt ggaagcacat ctagtagacc
      901 tttcctcatt gcatgtaagg gctgttctgc aactaccaac attctgtgtt ctgcgtattt
      961 ccgcacatac tcatatacat tctgtg
//
